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Traditional Chinese Medicine, the Zishen Yutai Pill, Ameliorates Precocious Endometrial Maturation Induced by Controlled Ovarian Hyperstimulation and Improves Uterine Receptivity via Upregulation of HOXA10.

Gao Q, Han L, Li X, Cai X - Evid Based Complement Alternat Med (2015)

Bottom Line: Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment.Elevation of HOXA10 led to further upregulation of its target gene, integrin β3, and downregulation of EMX2, two additional markers of uterine receptivity.In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830000, China.

ABSTRACT
Controlled ovarian hyperstimulation (COH) is widely used in assisted reproductive technology (ART), but it often leads to precocious maturation of the endometrium such that it impairs embryonic implantation and limits pregnancy rates. Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment. In the present study, the ZYP has been found to ameliorate precocious endometrial maturation in a mouse model of different COH. Molecular evaluations, real-time PCR, relative RT-PCR, Western blotting, and immunohistochemistry have indicated that the ZYP increased the expression of HOXA10, an important marker of uterine receptivity. Elevation of HOXA10 led to further upregulation of its target gene, integrin β3, and downregulation of EMX2, two additional markers of uterine receptivity. In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.

No MeSH data available.


Related in: MedlinePlus

Endometrial immunostaining results for HOXA10, integrin β3, and EMX2 protein levels of staining in mouse endometrium in each group. (a) Immunostaining. (b) Quantitative analysis of endometrial immunostaining.
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fig4: Endometrial immunostaining results for HOXA10, integrin β3, and EMX2 protein levels of staining in mouse endometrium in each group. (a) Immunostaining. (b) Quantitative analysis of endometrial immunostaining.

Mentions: Immunostaining results confirmed that HOXA10 protein was expressed in the luminal epithelium, glandular epithelium, and cytoplasm of mesenchymal cells. Integrin β3 protein was located in endometrial luminal epithelial cells and the cytoplasm of epithelial cells. EMX2 showed very weak expression in uterine epithelial cells and epithelial cells (Figure 4). Results showed that expression of HOXA10 and integrin β3 protein had diminished but that of EMX2 proteins were augmented by COH (Figure 4) in the endometrium of the current mouse model. These alterations were ameliorated by ZYP treatment, expression of HOXA10, and levels of integrin β3 protein had increased. Levels of EMX2 proteins decreased after ZYP treatment (Figure 4).


Traditional Chinese Medicine, the Zishen Yutai Pill, Ameliorates Precocious Endometrial Maturation Induced by Controlled Ovarian Hyperstimulation and Improves Uterine Receptivity via Upregulation of HOXA10.

Gao Q, Han L, Li X, Cai X - Evid Based Complement Alternat Med (2015)

Endometrial immunostaining results for HOXA10, integrin β3, and EMX2 protein levels of staining in mouse endometrium in each group. (a) Immunostaining. (b) Quantitative analysis of endometrial immunostaining.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352469&req=5

fig4: Endometrial immunostaining results for HOXA10, integrin β3, and EMX2 protein levels of staining in mouse endometrium in each group. (a) Immunostaining. (b) Quantitative analysis of endometrial immunostaining.
Mentions: Immunostaining results confirmed that HOXA10 protein was expressed in the luminal epithelium, glandular epithelium, and cytoplasm of mesenchymal cells. Integrin β3 protein was located in endometrial luminal epithelial cells and the cytoplasm of epithelial cells. EMX2 showed very weak expression in uterine epithelial cells and epithelial cells (Figure 4). Results showed that expression of HOXA10 and integrin β3 protein had diminished but that of EMX2 proteins were augmented by COH (Figure 4) in the endometrium of the current mouse model. These alterations were ameliorated by ZYP treatment, expression of HOXA10, and levels of integrin β3 protein had increased. Levels of EMX2 proteins decreased after ZYP treatment (Figure 4).

Bottom Line: Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment.Elevation of HOXA10 led to further upregulation of its target gene, integrin β3, and downregulation of EMX2, two additional markers of uterine receptivity.In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecology, Traditional Chinese Medical Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang 830000, China.

ABSTRACT
Controlled ovarian hyperstimulation (COH) is widely used in assisted reproductive technology (ART), but it often leads to precocious maturation of the endometrium such that it impairs embryonic implantation and limits pregnancy rates. Previous studies have shown the traditional Chinese medicine, the Zishen Yutai pill (ZYP), to be effective in treatment of threatened as well as recurrent miscarriages, and it can improve embryonic implantation rates in patients undergoing IVF treatment. In the present study, the ZYP has been found to ameliorate precocious endometrial maturation in a mouse model of different COH. Molecular evaluations, real-time PCR, relative RT-PCR, Western blotting, and immunohistochemistry have indicated that the ZYP increased the expression of HOXA10, an important marker of uterine receptivity. Elevation of HOXA10 led to further upregulation of its target gene, integrin β3, and downregulation of EMX2, two additional markers of uterine receptivity. In this way, the ZYP may mitigate COH-induced precocious maturation of the endometrium and improve uterine receptivity by upregulating HOXA10.

No MeSH data available.


Related in: MedlinePlus