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Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

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Related in: MedlinePlus

Heatmaps of fecal (a) and plasma (b) samples matrixes, considering all the quantitative variables measured and the categorized variables that were explained in the correspondent RDA, were performed. Clustering functions were applied to samples and variables after scaling the whole data set. In order to represent as much information as possible in the plot, the heatmaps were plotted using the measured data matrix scaled per variable and columns were labeled per infant and sampling time.
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Related In: Results  -  Collection


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fig4: Heatmaps of fecal (a) and plasma (b) samples matrixes, considering all the quantitative variables measured and the categorized variables that were explained in the correspondent RDA, were performed. Clustering functions were applied to samples and variables after scaling the whole data set. In order to represent as much information as possible in the plot, the heatmaps were plotted using the measured data matrix scaled per variable and columns were labeled per infant and sampling time.

Mentions: Those clinical categorical variables explained by the fecal and plasma RDAs were used, together with the microbiological, immunological, and clinical parameters, to create two heatmaps, one for each type of samples (Figure 4). The results from all the available fecal samples of the 5 infants were used to perform the heatmap showed in Figure 4(a). The samples' dendrogram shows two arms which clearly separate meconium and feces. The variables' dendrogram, obtained after samples clustering, shows two principal arms. The lower one is divided into two: the first of them that included clinical variables, some bacterial genera such as Escherichia, Staphylococcus, Bifidobacterium, and Paenibacillus, immunoglobulins IgG3 and IgG4, and cytokines IL-4, IL-13, and IL-2 and the second one that included antibiotherapy, IgG1, IL-5, IL-6, and IL-7. The upper arm is also divided and included the rest of the bacterial genera and immunological parameters together with the weight of the infants. The results obtained for all the available plasma samples from the 5 participants were used to perform the heatmap showed in Figure 4(b). The plasma samples' dendrogram shows two groups, in one of them 2 samples of the infant 2 cluster together with her twin at day 14 and samples of infant 5 clusters together with sample of infant 1 at day 7. In the second arm, siblings 3 and 4 at day 14 of probiotic supplementation initiate the clustering, which ends with sample of day 7 of infant 5 and sample of day 19 of infant 4 as previously observed in Figure 3. The dendrogram related to variables, obtained after infants clustering, showed two principal arms: one of them included clinical variables, hematological parameters, calprotectin, IL-1β, IL-4, IL-13, immunoglobulins IgA and IgG3, ibuprofen doses, and Hb and the second principal arm also divided including most of the cytokines, chemokines, and growth factors, the rest of the immunoglobulins, the birth weight, and the Hcte.


Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Heatmaps of fecal (a) and plasma (b) samples matrixes, considering all the quantitative variables measured and the categorized variables that were explained in the correspondent RDA, were performed. Clustering functions were applied to samples and variables after scaling the whole data set. In order to represent as much information as possible in the plot, the heatmaps were plotted using the measured data matrix scaled per variable and columns were labeled per infant and sampling time.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352454&req=5

fig4: Heatmaps of fecal (a) and plasma (b) samples matrixes, considering all the quantitative variables measured and the categorized variables that were explained in the correspondent RDA, were performed. Clustering functions were applied to samples and variables after scaling the whole data set. In order to represent as much information as possible in the plot, the heatmaps were plotted using the measured data matrix scaled per variable and columns were labeled per infant and sampling time.
Mentions: Those clinical categorical variables explained by the fecal and plasma RDAs were used, together with the microbiological, immunological, and clinical parameters, to create two heatmaps, one for each type of samples (Figure 4). The results from all the available fecal samples of the 5 infants were used to perform the heatmap showed in Figure 4(a). The samples' dendrogram shows two arms which clearly separate meconium and feces. The variables' dendrogram, obtained after samples clustering, shows two principal arms. The lower one is divided into two: the first of them that included clinical variables, some bacterial genera such as Escherichia, Staphylococcus, Bifidobacterium, and Paenibacillus, immunoglobulins IgG3 and IgG4, and cytokines IL-4, IL-13, and IL-2 and the second one that included antibiotherapy, IgG1, IL-5, IL-6, and IL-7. The upper arm is also divided and included the rest of the bacterial genera and immunological parameters together with the weight of the infants. The results obtained for all the available plasma samples from the 5 participants were used to perform the heatmap showed in Figure 4(b). The plasma samples' dendrogram shows two groups, in one of them 2 samples of the infant 2 cluster together with her twin at day 14 and samples of infant 5 clusters together with sample of infant 1 at day 7. In the second arm, siblings 3 and 4 at day 14 of probiotic supplementation initiate the clustering, which ends with sample of day 7 of infant 5 and sample of day 19 of infant 4 as previously observed in Figure 3. The dendrogram related to variables, obtained after infants clustering, showed two principal arms: one of them included clinical variables, hematological parameters, calprotectin, IL-1β, IL-4, IL-13, immunoglobulins IgA and IgG3, ibuprofen doses, and Hb and the second principal arm also divided including most of the cytokines, chemokines, and growth factors, the rest of the immunoglobulins, the birth weight, and the Hcte.

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

Show MeSH
Related in: MedlinePlus