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Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

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Redundancy analysis of the fecal samples obtained at different sampling times from the preterm infants. Cases were represented with points and then labeled per infant (1: circle, 2: square, 3: diamond, 4: triangle, and 5: inverted triangle) and sampling time (0: medium violet red, 7: green, 14: midnight blue, and 21: sky blue) Quantitative variables matrix, including the hematological and immunological parameters, ibuprofen doses (Ibu.doses), number of stools per day (N°.stools), and weight, was represented with each variable name or abbreviator in dark red color; clinical categorized observations vectors matrixes were used as constrained variables (airway resume (AWResume), antibiotherapy (Antibiotics), C-RP, ibuprofen treatment (Ibu treatment), nutrition type (Nutrition), patent ductus arteriosus (PDA), Sepsis, spontaneous stools (Spont.stools), and Transfusion) and represented as vectors in green color. The bidimensional RDA plot explains the 33% of the variability and showed a P value of 0.020 after 299 permutations when ANOVA test of the model was performed.
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fig2: Redundancy analysis of the fecal samples obtained at different sampling times from the preterm infants. Cases were represented with points and then labeled per infant (1: circle, 2: square, 3: diamond, 4: triangle, and 5: inverted triangle) and sampling time (0: medium violet red, 7: green, 14: midnight blue, and 21: sky blue) Quantitative variables matrix, including the hematological and immunological parameters, ibuprofen doses (Ibu.doses), number of stools per day (N°.stools), and weight, was represented with each variable name or abbreviator in dark red color; clinical categorized observations vectors matrixes were used as constrained variables (airway resume (AWResume), antibiotherapy (Antibiotics), C-RP, ibuprofen treatment (Ibu treatment), nutrition type (Nutrition), patent ductus arteriosus (PDA), Sepsis, spontaneous stools (Spont.stools), and Transfusion) and represented as vectors in green color. The bidimensional RDA plot explains the 33% of the variability and showed a P value of 0.020 after 299 permutations when ANOVA test of the model was performed.

Mentions: The redundancy analysis (RDA) of the above-mentioned variables for fecal samples is shown in Figure 2. The obtained model explains the 33% of the variability and the ANOVA test of the model was statistically significant (P = 0.020). The meconium samples were located opposite to microbial growth and in coincidence with the constrained antibiotic vector. Although the rest of fecal samples showed a less clear separation, the evolution of microbial colonization can be observed along the RDA1 axis in coincidence with the constrained vectors for AWResume, Nutrition, Spont.stools, PDA, and Transfusion and in opposite not only with the antibiotics and C-RP vectors, but also with the coordinates of proinflammatory compounds, such as calprotectin, MCP-1, MIP-1β, TNF-α, and IL-8 (Figure 2).


Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Redundancy analysis of the fecal samples obtained at different sampling times from the preterm infants. Cases were represented with points and then labeled per infant (1: circle, 2: square, 3: diamond, 4: triangle, and 5: inverted triangle) and sampling time (0: medium violet red, 7: green, 14: midnight blue, and 21: sky blue) Quantitative variables matrix, including the hematological and immunological parameters, ibuprofen doses (Ibu.doses), number of stools per day (N°.stools), and weight, was represented with each variable name or abbreviator in dark red color; clinical categorized observations vectors matrixes were used as constrained variables (airway resume (AWResume), antibiotherapy (Antibiotics), C-RP, ibuprofen treatment (Ibu treatment), nutrition type (Nutrition), patent ductus arteriosus (PDA), Sepsis, spontaneous stools (Spont.stools), and Transfusion) and represented as vectors in green color. The bidimensional RDA plot explains the 33% of the variability and showed a P value of 0.020 after 299 permutations when ANOVA test of the model was performed.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352454&req=5

fig2: Redundancy analysis of the fecal samples obtained at different sampling times from the preterm infants. Cases were represented with points and then labeled per infant (1: circle, 2: square, 3: diamond, 4: triangle, and 5: inverted triangle) and sampling time (0: medium violet red, 7: green, 14: midnight blue, and 21: sky blue) Quantitative variables matrix, including the hematological and immunological parameters, ibuprofen doses (Ibu.doses), number of stools per day (N°.stools), and weight, was represented with each variable name or abbreviator in dark red color; clinical categorized observations vectors matrixes were used as constrained variables (airway resume (AWResume), antibiotherapy (Antibiotics), C-RP, ibuprofen treatment (Ibu treatment), nutrition type (Nutrition), patent ductus arteriosus (PDA), Sepsis, spontaneous stools (Spont.stools), and Transfusion) and represented as vectors in green color. The bidimensional RDA plot explains the 33% of the variability and showed a P value of 0.020 after 299 permutations when ANOVA test of the model was performed.
Mentions: The redundancy analysis (RDA) of the above-mentioned variables for fecal samples is shown in Figure 2. The obtained model explains the 33% of the variability and the ANOVA test of the model was statistically significant (P = 0.020). The meconium samples were located opposite to microbial growth and in coincidence with the constrained antibiotic vector. Although the rest of fecal samples showed a less clear separation, the evolution of microbial colonization can be observed along the RDA1 axis in coincidence with the constrained vectors for AWResume, Nutrition, Spont.stools, PDA, and Transfusion and in opposite not only with the antibiotics and C-RP vectors, but also with the coordinates of proinflammatory compounds, such as calprotectin, MCP-1, MIP-1β, TNF-α, and IL-8 (Figure 2).

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

Show MeSH
Related in: MedlinePlus