Limits...
Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

Show MeSH

Related in: MedlinePlus

Phyla (a), genera (b), and bacterial diversity assessment by the SDI (c) of the microbiota of the meconium and fecal samples analyzed in this study. The relative contributions of the phyla and genera to the microbiota of the infant's gut and the SDI values were labeled per case and sampling time.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352454&req=5

fig1: Phyla (a), genera (b), and bacterial diversity assessment by the SDI (c) of the microbiota of the meconium and fecal samples analyzed in this study. The relative contributions of the phyla and genera to the microbiota of the infant's gut and the SDI values were labeled per case and sampling time.

Mentions: Globally, the phylum Firmicutes predominated in all the fecal samples except in those belonging to infant 5 where Proteobacteria was present in a similar proportion (Figure 1(a)). On the other hand, Proteobacteria dominated at the 14th day of intervention in fecal samples of the siblings 3 and 4. The phylum Actinobacteria, mainly represented by the genus Bifidobacterium, was isolated from day 7 although not in all the fecal samples (Figure 1(a)).


Administration of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934, two strains isolated from human milk, to very low and extremely low birth weight preterm infants: a pilot study.

Moles L, Escribano E, de Andrés J, Montes MT, Rodríguez JM, Jiménez E, Sáenz de Pipaón M, Espinosa-Martos I - J Immunol Res (2015)

Phyla (a), genera (b), and bacterial diversity assessment by the SDI (c) of the microbiota of the meconium and fecal samples analyzed in this study. The relative contributions of the phyla and genera to the microbiota of the infant's gut and the SDI values were labeled per case and sampling time.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352454&req=5

fig1: Phyla (a), genera (b), and bacterial diversity assessment by the SDI (c) of the microbiota of the meconium and fecal samples analyzed in this study. The relative contributions of the phyla and genera to the microbiota of the infant's gut and the SDI values were labeled per case and sampling time.
Mentions: Globally, the phylum Firmicutes predominated in all the fecal samples except in those belonging to infant 5 where Proteobacteria was present in a similar proportion (Figure 1(a)). On the other hand, Proteobacteria dominated at the 14th day of intervention in fecal samples of the siblings 3 and 4. The phylum Actinobacteria, mainly represented by the genus Bifidobacterium, was isolated from day 7 although not in all the fecal samples (Figure 1(a)).

Bottom Line: Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population.Bacterial growth was detected by culture-dependent techniques in all the fecal samples.Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

View Article: PubMed Central - PubMed

Affiliation: Departamento Nutrición, Bromatología y Tecnología de los Alimentos, Universidad Complutense de Madrid, 28040 Madrid, Spain.

ABSTRACT
The preterm infant gut has been described as immature and colonized by an aberrant microbiota. Therefore, the use of probiotics is an attractive practice in hospitals to try to reduce morbidity and mortality in this population. The objective of this pilot study was to elucidate if administration of two probiotic strains isolated from human milk to preterm infants led to their presence in feces. In addition, the evolution of a wide spectrum of immunological compounds, including the inflammatory biomarker calprotectin, in both blood and fecal samples was also assessed. For this purpose, five preterm infants received two daily doses (~10(9) CFU) of a 1:1 mixture of Bifidobacterium breve PS12929 and Lactobacillus salivarius PS12934. Bacterial growth was detected by culture-dependent techniques in all the fecal samples. The phylum Firmicutes dominated in nearly all fecal samples while L. salivarius PS12934 was detected in all the infants at numerous sample collection points and B. breve PS12929 appeared in five fecal samples. Finally, a noticeable decrease in the fecal calprotectin levels was observed along time.

Show MeSH
Related in: MedlinePlus