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Encapsulation of curcumin in diblock copolymer micelles for cancer therapy.

Alizadeh AM, Sadeghizadeh M, Najafi F, Ardestani SK, Erfani-Moghadam V, Khaniki M, Rezaei A, Zamani M, Khodayari S, Khodayari H, Mohagheghi MA - Biomed Res Int (2015)

Bottom Line: In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05).These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models.Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.

ABSTRACT
Application of nanoparticles has recently promising results for water insoluble agents like curcumin. In this study, we synthesized polymeric nanoparticle-curcumin (PNPC) and then showed its efficiency, drug loading, stability, and safety. Therapeutic effects of PNPC were also assessed on two cell lines and in an animal model of breast cancer. PNPC remarkably suppressed mammary and hepatocellular carcinoma cells proliferation (P < 0.05). Under the dosing procedure, PNPC was safe at 31.25 mg/kg and lower doses. Higher doses demonstrated minimal hepatocellular and renal toxicity in paraclinical and histopathological examinations. Tumor take rate in PNPC-treated group was 37.5% compared with 87.5% in control (P < 0.05). Average tumor size and weight were significantly lower in PNPC group than control (P < 0.05). PNPC increased proapoptotic Bax protein expression (P < 0.05). Antiapoptotic Bcl-2 protein expression, however, was lower in PNPC-treated animals than the control ones (P < 0.05). In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05). These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models. Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

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Related in: MedlinePlus

The polymeric nanoparticle curcumin effects on immunohistochemical markers in mouse mammary tumor. Data reported are mean ± SD; *P < 0.05 compared to control; PNPC = polymeric nanoparticle curcumin.
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fig8: The polymeric nanoparticle curcumin effects on immunohistochemical markers in mouse mammary tumor. Data reported are mean ± SD; *P < 0.05 compared to control; PNPC = polymeric nanoparticle curcumin.

Mentions: Immunohistochemistry examinations in PNPC-treated animals showed increased proapoptotic Bax protein expression in breast tumor in comparison with control (Figure 8 and Supplemental Figure  7A). Antiapoptotic Bcl-2 protein expression conceivably lowered after PNPC therapy (Figure 8 and Supplemental Figure  7B). In addition, treatment with PNPC caused a significant reduction in Bcl-2 activity and Bcl-2/Bax ratio compared to the control group (P < 0.05).


Encapsulation of curcumin in diblock copolymer micelles for cancer therapy.

Alizadeh AM, Sadeghizadeh M, Najafi F, Ardestani SK, Erfani-Moghadam V, Khaniki M, Rezaei A, Zamani M, Khodayari S, Khodayari H, Mohagheghi MA - Biomed Res Int (2015)

The polymeric nanoparticle curcumin effects on immunohistochemical markers in mouse mammary tumor. Data reported are mean ± SD; *P < 0.05 compared to control; PNPC = polymeric nanoparticle curcumin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352453&req=5

fig8: The polymeric nanoparticle curcumin effects on immunohistochemical markers in mouse mammary tumor. Data reported are mean ± SD; *P < 0.05 compared to control; PNPC = polymeric nanoparticle curcumin.
Mentions: Immunohistochemistry examinations in PNPC-treated animals showed increased proapoptotic Bax protein expression in breast tumor in comparison with control (Figure 8 and Supplemental Figure  7A). Antiapoptotic Bcl-2 protein expression conceivably lowered after PNPC therapy (Figure 8 and Supplemental Figure  7B). In addition, treatment with PNPC caused a significant reduction in Bcl-2 activity and Bcl-2/Bax ratio compared to the control group (P < 0.05).

Bottom Line: In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05).These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models.Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.

ABSTRACT
Application of nanoparticles has recently promising results for water insoluble agents like curcumin. In this study, we synthesized polymeric nanoparticle-curcumin (PNPC) and then showed its efficiency, drug loading, stability, and safety. Therapeutic effects of PNPC were also assessed on two cell lines and in an animal model of breast cancer. PNPC remarkably suppressed mammary and hepatocellular carcinoma cells proliferation (P < 0.05). Under the dosing procedure, PNPC was safe at 31.25 mg/kg and lower doses. Higher doses demonstrated minimal hepatocellular and renal toxicity in paraclinical and histopathological examinations. Tumor take rate in PNPC-treated group was 37.5% compared with 87.5% in control (P < 0.05). Average tumor size and weight were significantly lower in PNPC group than control (P < 0.05). PNPC increased proapoptotic Bax protein expression (P < 0.05). Antiapoptotic Bcl-2 protein expression, however, was lower in PNPC-treated animals than the control ones (P < 0.05). In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05). These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models. Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

Show MeSH
Related in: MedlinePlus