Limits...
Encapsulation of curcumin in diblock copolymer micelles for cancer therapy.

Alizadeh AM, Sadeghizadeh M, Najafi F, Ardestani SK, Erfani-Moghadam V, Khaniki M, Rezaei A, Zamani M, Khodayari S, Khodayari H, Mohagheghi MA - Biomed Res Int (2015)

Bottom Line: In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05).These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models.Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.

ABSTRACT
Application of nanoparticles has recently promising results for water insoluble agents like curcumin. In this study, we synthesized polymeric nanoparticle-curcumin (PNPC) and then showed its efficiency, drug loading, stability, and safety. Therapeutic effects of PNPC were also assessed on two cell lines and in an animal model of breast cancer. PNPC remarkably suppressed mammary and hepatocellular carcinoma cells proliferation (P < 0.05). Under the dosing procedure, PNPC was safe at 31.25 mg/kg and lower doses. Higher doses demonstrated minimal hepatocellular and renal toxicity in paraclinical and histopathological examinations. Tumor take rate in PNPC-treated group was 37.5% compared with 87.5% in control (P < 0.05). Average tumor size and weight were significantly lower in PNPC group than control (P < 0.05). PNPC increased proapoptotic Bax protein expression (P < 0.05). Antiapoptotic Bcl-2 protein expression, however, was lower in PNPC-treated animals than the control ones (P < 0.05). In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05). These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models. Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

Show MeSH

Related in: MedlinePlus

Stability test of curcumin/nanoparticle after one week at room temperature by DLS test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352453&req=5

fig4: Stability test of curcumin/nanoparticle after one week at room temperature by DLS test.

Mentions: PNPC was stable more than 300 days at 4°C, although loading curcumin higher than 6% into nanoparticles leads to some precipitation of curcumin (Supplemental Figure  7). Lyophilized PNPC samples in water dissolved by manual shaking without additional heating or sonication. Then, size and distribution of PNP were compared with freshly prepared PNPC by DLS (Figure 2(a)). After one week at 25°C, as mentioned, PNPC samples inclined to develop more uniform polymersomes nanoparticles (PdI = 0.182 ± 0.072). Furthermore, spectroscopy analysis showed that one-third of curcumin in curcumin/nanocarriers micelles (pH = 7.2) was conserved at room temperature after one week (Figure 4).


Encapsulation of curcumin in diblock copolymer micelles for cancer therapy.

Alizadeh AM, Sadeghizadeh M, Najafi F, Ardestani SK, Erfani-Moghadam V, Khaniki M, Rezaei A, Zamani M, Khodayari S, Khodayari H, Mohagheghi MA - Biomed Res Int (2015)

Stability test of curcumin/nanoparticle after one week at room temperature by DLS test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352453&req=5

fig4: Stability test of curcumin/nanoparticle after one week at room temperature by DLS test.
Mentions: PNPC was stable more than 300 days at 4°C, although loading curcumin higher than 6% into nanoparticles leads to some precipitation of curcumin (Supplemental Figure  7). Lyophilized PNPC samples in water dissolved by manual shaking without additional heating or sonication. Then, size and distribution of PNP were compared with freshly prepared PNPC by DLS (Figure 2(a)). After one week at 25°C, as mentioned, PNPC samples inclined to develop more uniform polymersomes nanoparticles (PdI = 0.182 ± 0.072). Furthermore, spectroscopy analysis showed that one-third of curcumin in curcumin/nanocarriers micelles (pH = 7.2) was conserved at room temperature after one week (Figure 4).

Bottom Line: In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05).These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models.Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

View Article: PubMed Central - PubMed

Affiliation: Cancer Research Center, Tehran University of Medical Sciences, Tehran 14197-33141, Iran.

ABSTRACT
Application of nanoparticles has recently promising results for water insoluble agents like curcumin. In this study, we synthesized polymeric nanoparticle-curcumin (PNPC) and then showed its efficiency, drug loading, stability, and safety. Therapeutic effects of PNPC were also assessed on two cell lines and in an animal model of breast cancer. PNPC remarkably suppressed mammary and hepatocellular carcinoma cells proliferation (P < 0.05). Under the dosing procedure, PNPC was safe at 31.25 mg/kg and lower doses. Higher doses demonstrated minimal hepatocellular and renal toxicity in paraclinical and histopathological examinations. Tumor take rate in PNPC-treated group was 37.5% compared with 87.5% in control (P < 0.05). Average tumor size and weight were significantly lower in PNPC group than control (P < 0.05). PNPC increased proapoptotic Bax protein expression (P < 0.05). Antiapoptotic Bcl-2 protein expression, however, was lower in PNPC-treated animals than the control ones (P < 0.05). In addition, proliferative and angiogenic parameters were statistically decreased in PNPC-treated animals (P < 0.05). These results highlight the suppressing role for PNPC in in vitro and in vivo tumor growth models. Our findings provide credible evidence for superior biocompatibility of the polymeric nanocarrier in pharmacological arena together with an excellent tumor-suppressing response.

Show MeSH
Related in: MedlinePlus