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Daily intake of probiotics with high IFN-γ/IL-10 ratio increases the cytotoxicity of human natural killer cells: a personalized probiotic approach.

Ho YH, Lu YC, Chang HC, Lee SY, Tsai MF, Huang YT, Hsu TY - J Immunol Res (2014)

Bottom Line: A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101.People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group.The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Research and Development, Bio Ray Biotech Inc., 3F., No. 466, Bo'ai 1st Road, Gushan District, Kaohsiung City 80466, Taiwan.

ABSTRACT
A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-γ group was observed compared to the IL-10 group (n = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.

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Related in: MedlinePlus

Distribution of the probiotic effects on the NK cytotoxicity variations. (a) IFN-γ group. (b) IL-10 group. Some probiotics exhibited good potential to enhance the NK activity, such as E. faecium BR0085 in both groups. The probiotics in IFN-γ group possessed a significant capability to increase the NK cytotoxicity compared to the IL-10 group.
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fig2: Distribution of the probiotic effects on the NK cytotoxicity variations. (a) IFN-γ group. (b) IL-10 group. Some probiotics exhibited good potential to enhance the NK activity, such as E. faecium BR0085 in both groups. The probiotics in IFN-γ group possessed a significant capability to increase the NK cytotoxicity compared to the IL-10 group.

Mentions: Besides the analysis of occurrence of each probiotic, Table 3 also displays the overall performance of 6 probiotics on the NK activity. As mentioned in previous section, IFN-γ group mediated a significant increase in NK cytotoxicity but not in IL-10 group. It is clear that the average NK cytotoxicity variations of 5 strains in IFN-γ group were all positive (except for L. reuteri BR101, which was zero occurrences). In the IL-10 group, only E. faecium BR0085 showed greater ability to increase the NK cytotoxicity and the other strains were all below the average value (1.4%). Some of them were even negatively correlated with the NK cytotoxicity tendency (B. longum BA100: −1.33%, L. rhamnosus AD500: −7.66%). Detailed variations of the probiotics effects on NK activity were displayed in Figure 2(a) (IFN-γ group) and Figure 2(b) (IL-10 group).


Daily intake of probiotics with high IFN-γ/IL-10 ratio increases the cytotoxicity of human natural killer cells: a personalized probiotic approach.

Ho YH, Lu YC, Chang HC, Lee SY, Tsai MF, Huang YT, Hsu TY - J Immunol Res (2014)

Distribution of the probiotic effects on the NK cytotoxicity variations. (a) IFN-γ group. (b) IL-10 group. Some probiotics exhibited good potential to enhance the NK activity, such as E. faecium BR0085 in both groups. The probiotics in IFN-γ group possessed a significant capability to increase the NK cytotoxicity compared to the IL-10 group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352450&req=5

fig2: Distribution of the probiotic effects on the NK cytotoxicity variations. (a) IFN-γ group. (b) IL-10 group. Some probiotics exhibited good potential to enhance the NK activity, such as E. faecium BR0085 in both groups. The probiotics in IFN-γ group possessed a significant capability to increase the NK cytotoxicity compared to the IL-10 group.
Mentions: Besides the analysis of occurrence of each probiotic, Table 3 also displays the overall performance of 6 probiotics on the NK activity. As mentioned in previous section, IFN-γ group mediated a significant increase in NK cytotoxicity but not in IL-10 group. It is clear that the average NK cytotoxicity variations of 5 strains in IFN-γ group were all positive (except for L. reuteri BR101, which was zero occurrences). In the IL-10 group, only E. faecium BR0085 showed greater ability to increase the NK cytotoxicity and the other strains were all below the average value (1.4%). Some of them were even negatively correlated with the NK cytotoxicity tendency (B. longum BA100: −1.33%, L. rhamnosus AD500: −7.66%). Detailed variations of the probiotics effects on NK activity were displayed in Figure 2(a) (IFN-γ group) and Figure 2(b) (IL-10 group).

Bottom Line: A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101.People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group.The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Research and Development, Bio Ray Biotech Inc., 3F., No. 466, Bo'ai 1st Road, Gushan District, Kaohsiung City 80466, Taiwan.

ABSTRACT
A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-γ group was observed compared to the IL-10 group (n = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.

Show MeSH
Related in: MedlinePlus