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Daily intake of probiotics with high IFN-γ/IL-10 ratio increases the cytotoxicity of human natural killer cells: a personalized probiotic approach.

Ho YH, Lu YC, Chang HC, Lee SY, Tsai MF, Huang YT, Hsu TY - J Immunol Res (2014)

Bottom Line: A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101.People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group.The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Research and Development, Bio Ray Biotech Inc., 3F., No. 466, Bo'ai 1st Road, Gushan District, Kaohsiung City 80466, Taiwan.

ABSTRACT
A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-γ group was observed compared to the IL-10 group (n = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.

Show MeSH

Related in: MedlinePlus

A figure of the microfluidic chip. After adding buffers to each well, the chip then went through the automatic process in a reaction chamber. The entire process was about 1 hour and then the chip can be taken out to the analyzer for determining the amount of IFN-γ and IL-10.
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fig1: A figure of the microfluidic chip. After adding buffers to each well, the chip then went through the automatic process in a reaction chamber. The entire process was about 1 hour and then the chip can be taken out to the analyzer for determining the amount of IFN-γ and IL-10.

Mentions: The first process of this study is to determine the personalized probiotics from six probiotic strains (L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101) for each participant by the microfluidic chip assay. The microfluidic chip was displayed in Figure 1; compared to the traditional ELISA process, this platform avoids the manual error because the reactions of each process were automatically performed. Thus, manual errors or influences can be limited. Typical microfluidic results are displayed in Table 1. This table displays the percentage of IFN-γ/IL-10 or IL-10/IFN-γ ratio of six probiotics for specific subjects. For instance, in the left part of Table 1, the subject took the L. paracasei BRAP01 as his own personalized probiotics, while, in the right part, the person took the L. reuteri BR101 for comparison. By doing so, people in IFN-γ group consumed the probiotics that had the highest effect on the production of IFN-γ and this condition was opposite in the IL-10 group. The purpose of this experimental design is to separate the subjects into two different groups and we want to clarify the influence on NK activity based on these two totally different approaches.


Daily intake of probiotics with high IFN-γ/IL-10 ratio increases the cytotoxicity of human natural killer cells: a personalized probiotic approach.

Ho YH, Lu YC, Chang HC, Lee SY, Tsai MF, Huang YT, Hsu TY - J Immunol Res (2014)

A figure of the microfluidic chip. After adding buffers to each well, the chip then went through the automatic process in a reaction chamber. The entire process was about 1 hour and then the chip can be taken out to the analyzer for determining the amount of IFN-γ and IL-10.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352450&req=5

fig1: A figure of the microfluidic chip. After adding buffers to each well, the chip then went through the automatic process in a reaction chamber. The entire process was about 1 hour and then the chip can be taken out to the analyzer for determining the amount of IFN-γ and IL-10.
Mentions: The first process of this study is to determine the personalized probiotics from six probiotic strains (L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101) for each participant by the microfluidic chip assay. The microfluidic chip was displayed in Figure 1; compared to the traditional ELISA process, this platform avoids the manual error because the reactions of each process were automatically performed. Thus, manual errors or influences can be limited. Typical microfluidic results are displayed in Table 1. This table displays the percentage of IFN-γ/IL-10 or IL-10/IFN-γ ratio of six probiotics for specific subjects. For instance, in the left part of Table 1, the subject took the L. paracasei BRAP01 as his own personalized probiotics, while, in the right part, the person took the L. reuteri BR101 for comparison. By doing so, people in IFN-γ group consumed the probiotics that had the highest effect on the production of IFN-γ and this condition was opposite in the IL-10 group. The purpose of this experimental design is to separate the subjects into two different groups and we want to clarify the influence on NK activity based on these two totally different approaches.

Bottom Line: A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101.People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group.The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Research and Development, Bio Ray Biotech Inc., 3F., No. 466, Bo'ai 1st Road, Gushan District, Kaohsiung City 80466, Taiwan.

ABSTRACT
A personalized probiotic microfluidic chip system has been established and used to screen the probiotics which had the highest value of IFN-γ/IL-10 or IL-10/IFN-γ among six probiotics, including L. paracasei BRAP01, L. acidophilus AD300, B. longum BA100, E. faecium BR0085, L. rhamnosus AD500, and L. reuteri BR101. One hundred volunteers were included and their PBMCs were collected and stimulated by the six probiotics. People who belonged to the IFN-γ group took the probiotics that exerted the highest ratio of IFN-γ/IL-10 and vice versa in IL-10 group. A significant increase in NK cytotoxicity of 69 volunteers in the IFN-γ group was observed compared to the IL-10 group (n = 21) and control group (n = 10). The result also showed that L. paracasei BRAP01 and L. acidophilus AD300 were the two dominant inducers in IFN-γ group which yielded higher value of IFN-γ/IL-10 than the other 4 probiotics, while L. reuteri BR101 was the most effective agent on the ratio of IL-10/IFN-γ in the IL-10 group. Our finding highlighted the concept of personalized probiotics and also provided a good foundation to investigate the probiotics with NK activity.

Show MeSH
Related in: MedlinePlus