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Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus

Hippocampus glial fibrillary acidic protein. (a) The pictures reflect the expression of GFAP in 4 groups (original magnification ×400). (A) represents normal control group (4.33 ± 1.52), (B) represents no treatment control group (13.90 ± 4.06), (C) represents sham moxibustion group (16.82 ± 5.23), and (D) represents moxibustion group (4.85 ± 1.89). Blue arrows identify examples of clusters of GFAP positive cells. (b) Bar graph represents GFAP immunoreactivity in the hippocampus CA3. *P < 0.05 versus no treatment control group; ΔP < 0.05 versus sham moxibustion group.
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fig4: Hippocampus glial fibrillary acidic protein. (a) The pictures reflect the expression of GFAP in 4 groups (original magnification ×400). (A) represents normal control group (4.33 ± 1.52), (B) represents no treatment control group (13.90 ± 4.06), (C) represents sham moxibustion group (16.82 ± 5.23), and (D) represents moxibustion group (4.85 ± 1.89). Blue arrows identify examples of clusters of GFAP positive cells. (b) Bar graph represents GFAP immunoreactivity in the hippocampus CA3. *P < 0.05 versus no treatment control group; ΔP < 0.05 versus sham moxibustion group.

Mentions: The optical density of GFAP in hippocampus expressed the highest level in no treatment control group, came second in sham moxibustion group, and was the least in moxibustion and normal control groups. Compared with normal control group and no treatment control group, moxibustion group and sham moxibustion group both showed significant differences (Figure 4).


Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

Hippocampus glial fibrillary acidic protein. (a) The pictures reflect the expression of GFAP in 4 groups (original magnification ×400). (A) represents normal control group (4.33 ± 1.52), (B) represents no treatment control group (13.90 ± 4.06), (C) represents sham moxibustion group (16.82 ± 5.23), and (D) represents moxibustion group (4.85 ± 1.89). Blue arrows identify examples of clusters of GFAP positive cells. (b) Bar graph represents GFAP immunoreactivity in the hippocampus CA3. *P < 0.05 versus no treatment control group; ΔP < 0.05 versus sham moxibustion group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352425&req=5

fig4: Hippocampus glial fibrillary acidic protein. (a) The pictures reflect the expression of GFAP in 4 groups (original magnification ×400). (A) represents normal control group (4.33 ± 1.52), (B) represents no treatment control group (13.90 ± 4.06), (C) represents sham moxibustion group (16.82 ± 5.23), and (D) represents moxibustion group (4.85 ± 1.89). Blue arrows identify examples of clusters of GFAP positive cells. (b) Bar graph represents GFAP immunoreactivity in the hippocampus CA3. *P < 0.05 versus no treatment control group; ΔP < 0.05 versus sham moxibustion group.
Mentions: The optical density of GFAP in hippocampus expressed the highest level in no treatment control group, came second in sham moxibustion group, and was the least in moxibustion and normal control groups. Compared with normal control group and no treatment control group, moxibustion group and sham moxibustion group both showed significant differences (Figure 4).

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus