Limits...
Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus

The performance of 4 groups in Morris water maze task. (a) Each point represents group mean latency (s) to reach the hidden platform in four groups during visible platform training. (b) Bar chart shows the percentage of time spent in each quadrant within 60 s during absence platform spatial memory test. (c) The Morris water maze pool is divided into 4 quadrants. The yellow circle represents the platform which is placed in training quadrant.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352425&req=5

fig3: The performance of 4 groups in Morris water maze task. (a) Each point represents group mean latency (s) to reach the hidden platform in four groups during visible platform training. (b) Bar chart shows the percentage of time spent in each quadrant within 60 s during absence platform spatial memory test. (c) The Morris water maze pool is divided into 4 quadrants. The yellow circle represents the platform which is placed in training quadrant.

Mentions: No significant difference was found among the groups. After 4 days of spatial learning, the memory retention of mice was recorded as the time searching for absence platform in training quadrant in percentage of 60 s. All groups except the no treatment control manifested longer retention in training quadrant, more than the total length of 25%. Among all these 4 quadrants, all groups except for no treatment control tended to stay longer in training quadrant rather than opposite and adjacent-R quadrants (Figure 3).


Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

The performance of 4 groups in Morris water maze task. (a) Each point represents group mean latency (s) to reach the hidden platform in four groups during visible platform training. (b) Bar chart shows the percentage of time spent in each quadrant within 60 s during absence platform spatial memory test. (c) The Morris water maze pool is divided into 4 quadrants. The yellow circle represents the platform which is placed in training quadrant.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352425&req=5

fig3: The performance of 4 groups in Morris water maze task. (a) Each point represents group mean latency (s) to reach the hidden platform in four groups during visible platform training. (b) Bar chart shows the percentage of time spent in each quadrant within 60 s during absence platform spatial memory test. (c) The Morris water maze pool is divided into 4 quadrants. The yellow circle represents the platform which is placed in training quadrant.
Mentions: No significant difference was found among the groups. After 4 days of spatial learning, the memory retention of mice was recorded as the time searching for absence platform in training quadrant in percentage of 60 s. All groups except the no treatment control manifested longer retention in training quadrant, more than the total length of 25%. Among all these 4 quadrants, all groups except for no treatment control tended to stay longer in training quadrant rather than opposite and adjacent-R quadrants (Figure 3).

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus