Limits...
Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus

Intervening measures.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352425&req=5

fig2: Intervening measures.

Mentions: Mice in normal and no treatment control groups were exposed without manipulations in room 3 free of moxa smoke (Figure 2).


Effects of shenque moxibustion on behavioral changes and brain oxidative state in apolipoprotein e-deficient mice.

Liu J, Zhao B, Cui Y, Huang Y, Huang C, Huang J, Han L, Lao L - Evid Based Complement Alternat Med (2015)

Intervening measures.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352425&req=5

fig2: Intervening measures.
Mentions: Mice in normal and no treatment control groups were exposed without manipulations in room 3 free of moxa smoke (Figure 2).

Bottom Line: The mice were then sacrificed and the tissues were harvested for immune-histochemical staining.Results.Conclusion.

View Article: PubMed Central - PubMed

Affiliation: School of Acupuncture and Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China.

ABSTRACT
Purpose. To determine whether moxibustion influences the learning and memory behavior of ApoE-/- male mice, and investigate the mechanism of moxibustion on the alteration of oxidized proteins (glial fibrillary acidic protein, β-amyloid) in hippocampus. Methods. Thirty-three ApoE-/- mice were randomly divided into 3 groups (n = 11/group): moxibustion, sham moxibustion, and no treatment control. Wild-type C57BL/6 mice (n = 13) were used for normal control. Moxibustion was performed with Shenque (RN8) moxibustion for 20 minutes per day, 6 days/week for 12 weeks. In sham control, the procedure was similar except burning of the moxa stick. Behavioral tests (step-down test and Morris water maze task) were conducted in the 13th week. The mice were then sacrificed and the tissues were harvested for immune-histochemical staining. Results. In the step-down test, the moxibustion group had shorter reaction time in training record and committed less mistakes compared to sham control. In immune-histochemical study, the moxibustion group expressed lower level of GFAP and less aggregation of β-amyloid in the hippocampus than the sham control. Conclusion. Our findings suggest that moxibustion may enhance learning capability of ApoE-/- mice. The mechanism may be via inhibiting oxidized proteins (GFAP and β-amyloid) in astrocytes.

No MeSH data available.


Related in: MedlinePlus