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Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.

Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, Magnusson U - Biomed Res Int (2015)

Bottom Line: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle.Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda.These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.

ABSTRACT
Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

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Related in: MedlinePlus

Bruce-ladder multiplex PCR agarose gel picture used to confirm the Brucella species isolated. Extreme left and right lanes are for 100 bp molecular weight marker; from left to right: lanes 2, 5–13 are for DNA from isolates detected as Brucella with strong Ct values; lanes 3, 4, and 14 are for DNA from Brucella-like isolates with weak Ct values; lanes 15 and 19 are for B. suis positive control DNA; lane 16 is for RB 51 positive control DNA; lane 17 is for Rev 1 positive control DNA; and lane 18 is for PCR grade water (negative control).
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Related In: Results  -  Collection


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fig4: Bruce-ladder multiplex PCR agarose gel picture used to confirm the Brucella species isolated. Extreme left and right lanes are for 100 bp molecular weight marker; from left to right: lanes 2, 5–13 are for DNA from isolates detected as Brucella with strong Ct values; lanes 3, 4, and 14 are for DNA from Brucella-like isolates with weak Ct values; lanes 15 and 19 are for B. suis positive control DNA; lane 16 is for RB 51 positive control DNA; lane 17 is for Rev 1 positive control DNA; and lane 18 is for PCR grade water (negative control).

Mentions: DNA from all the 11 isolates that were confirmed as belonging to the genus Brucella in the triplex real-time PCR was detected as B. abortus DNA by the Bruce-ladder multiplex PCR (Figure 4). B. abortus gives two bands of 1682 bp and 587 bp on Bruce-ladder PCR.


Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.

Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, Magnusson U - Biomed Res Int (2015)

Bruce-ladder multiplex PCR agarose gel picture used to confirm the Brucella species isolated. Extreme left and right lanes are for 100 bp molecular weight marker; from left to right: lanes 2, 5–13 are for DNA from isolates detected as Brucella with strong Ct values; lanes 3, 4, and 14 are for DNA from Brucella-like isolates with weak Ct values; lanes 15 and 19 are for B. suis positive control DNA; lane 16 is for RB 51 positive control DNA; lane 17 is for Rev 1 positive control DNA; and lane 18 is for PCR grade water (negative control).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352423&req=5

fig4: Bruce-ladder multiplex PCR agarose gel picture used to confirm the Brucella species isolated. Extreme left and right lanes are for 100 bp molecular weight marker; from left to right: lanes 2, 5–13 are for DNA from isolates detected as Brucella with strong Ct values; lanes 3, 4, and 14 are for DNA from Brucella-like isolates with weak Ct values; lanes 15 and 19 are for B. suis positive control DNA; lane 16 is for RB 51 positive control DNA; lane 17 is for Rev 1 positive control DNA; and lane 18 is for PCR grade water (negative control).
Mentions: DNA from all the 11 isolates that were confirmed as belonging to the genus Brucella in the triplex real-time PCR was detected as B. abortus DNA by the Bruce-ladder multiplex PCR (Figure 4). B. abortus gives two bands of 1682 bp and 587 bp on Bruce-ladder PCR.

Bottom Line: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle.Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda.These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.

ABSTRACT
Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

Show MeSH
Related in: MedlinePlus