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Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.

Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, Magnusson U - Biomed Res Int (2015)

Bottom Line: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle.Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda.These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.

ABSTRACT
Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

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Related in: MedlinePlus

Triplex real-time PCR amplification pattern using the B. abortus probe. Fluorescence ratio is plotted against the number of PCR cycles to monitor amplification in real-time mode. Only B. abortus (positive control) was picked with a strong Ct value by this probe.
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Related In: Results  -  Collection


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fig3: Triplex real-time PCR amplification pattern using the B. abortus probe. Fluorescence ratio is plotted against the number of PCR cycles to monitor amplification in real-time mode. Only B. abortus (positive control) was picked with a strong Ct value by this probe.

Mentions: DNA from all the 11 isolates that was judged as B. abortus by the biotyping was detected as Brucella DNA by the Brucella genus probe in the triplex real-time PCR (Figure 1). However the triplex real-time PCR was unable to detect the Brucella species involved using its B. melitensis probe (Figure 2) and B. abortus probe (Figure 3).


Isolation and molecular characterization of Brucella isolates in cattle milk in Uganda.

Mugizi DR, Muradrasoli S, Boqvist S, Erume J, Nasinyama GW, Waiswa C, Mboowa G, Klint M, Magnusson U - Biomed Res Int (2015)

Triplex real-time PCR amplification pattern using the B. abortus probe. Fluorescence ratio is plotted against the number of PCR cycles to monitor amplification in real-time mode. Only B. abortus (positive control) was picked with a strong Ct value by this probe.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352423&req=5

fig3: Triplex real-time PCR amplification pattern using the B. abortus probe. Fluorescence ratio is plotted against the number of PCR cycles to monitor amplification in real-time mode. Only B. abortus (positive control) was picked with a strong Ct value by this probe.
Mentions: DNA from all the 11 isolates that was judged as B. abortus by the biotyping was detected as Brucella DNA by the Brucella genus probe in the triplex real-time PCR (Figure 1). However the triplex real-time PCR was unable to detect the Brucella species involved using its B. melitensis probe (Figure 2) and B. abortus probe (Figure 3).

Bottom Line: Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle.Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda.These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Animal Resources and Bio-Security, Makerere University, P.O. Box 7062, Kampala, Uganda.

ABSTRACT
Brucellosis is endemic in livestock and humans in Uganda and its transmission involves a multitude of risk factors like consumption of milk from infected cattle. To shed new light on the epidemiology of brucellosis in Uganda the present study used phenotypic and molecular approaches to delineate the Brucella species, biovars, and genotypes shed in cattle milk. Brucella abortus without a biovar designation was isolated from eleven out of 207 milk samples from cattle in Uganda. These isolates had a genomic monomorphism at 16 variable number tandem repeat (VNTR) loci and showed in turn high levels of genetic variation when compared with other African strains or other B. abortus biovars from other parts of the world. This study further highlights the usefulness of MLVA as an epidemiological tool for investigation of Brucella infections.

Show MeSH
Related in: MedlinePlus