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Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry.

Muradoglu F, Gundogdu M, Ercisli S, Encu T, Balta F, Jaafar HZ, Zia-Ul-Haq M - Biol. Res. (2015)

Bottom Line: Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves.The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry.We found that MDA and antioxidant enzyme (CAT, SOD and APX) contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Cadmium (Cd) is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA) contents, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd.

Results: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves. SOD and CAT activities was also increased with the increase Cd concentrations. K, Mn and Mg concentrations were found higher in leaves than roots under Cd stress. In general, increased Cd treatments increased K, Mg, Fe, Ca, Cu and Zn concentration in both roots and leaves. Excessive Cd treatments reduced chlorophyll contents, increased antioxidant enzyme activities and changes in plant nutrition concentrations in both roots and leaves.

Conclusion: The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry. Strawberry roots accumulated higher Cd than leaves. We found that MDA and antioxidant enzyme (CAT, SOD and APX) contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

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Changes of chlorophyll a and b contents exposed to different Cd applications in leaves of strawberry plants. Same letters are not significantly different according to Duncan test (p≤0.05).
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Fig1: Changes of chlorophyll a and b contents exposed to different Cd applications in leaves of strawberry plants. Same letters are not significantly different according to Duncan test (p≤0.05).

Mentions: As shown in Figure 1. The chlorophyll content in strawberry plant organs decreased under Cd treatment. There was regularly a reduction attributable to Cd application both chlorophyll a and chlorophyll b in Camarosa (strawberry) cultivar. Chlorophyll a content was found higher than chlorophyll b content. There was nearly 5, 15, 25, and 30% decrease in chlorophyll a and 3, 11, 15 and 18% decrease in chlorophyll b when Cd applications were increased from 0 to 60 mg kg−1 respectively. According to Qian et al. [19], cadmium-induced declining effect on chlorophyll and carotenoid contents which could be explained on the basis of inhibitory effect of Cd on enzymes involved in pigment biosynthesis. Furthermore, chlorophyll a and chlorophyll b contents showed significant decline at the applications of Cd and the results were in consist with earlier report where Cd inhibited the biosynthesis of chlorophyll and generated a kind of senescence [19,20]. Our results are in agreement with finding by Yang et al. [21] who reported that leaves of Potamogeton crispus under Cd stress showed decreased 35,8% chlorophyll a and 26.7% chlorophyll b and chlorophyll a content was found higher than chlorophyll b content. Several report have shown that under Cd stress decrease chlorophyll content in leaf garden grass [22] and almond seedling [23]. Therefore, chlorophyll pigments seem to be one of the main reasons of heavy-metal injury in plants.Figure 1


Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry.

Muradoglu F, Gundogdu M, Ercisli S, Encu T, Balta F, Jaafar HZ, Zia-Ul-Haq M - Biol. Res. (2015)

Changes of chlorophyll a and b contents exposed to different Cd applications in leaves of strawberry plants. Same letters are not significantly different according to Duncan test (p≤0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352267&req=5

Fig1: Changes of chlorophyll a and b contents exposed to different Cd applications in leaves of strawberry plants. Same letters are not significantly different according to Duncan test (p≤0.05).
Mentions: As shown in Figure 1. The chlorophyll content in strawberry plant organs decreased under Cd treatment. There was regularly a reduction attributable to Cd application both chlorophyll a and chlorophyll b in Camarosa (strawberry) cultivar. Chlorophyll a content was found higher than chlorophyll b content. There was nearly 5, 15, 25, and 30% decrease in chlorophyll a and 3, 11, 15 and 18% decrease in chlorophyll b when Cd applications were increased from 0 to 60 mg kg−1 respectively. According to Qian et al. [19], cadmium-induced declining effect on chlorophyll and carotenoid contents which could be explained on the basis of inhibitory effect of Cd on enzymes involved in pigment biosynthesis. Furthermore, chlorophyll a and chlorophyll b contents showed significant decline at the applications of Cd and the results were in consist with earlier report where Cd inhibited the biosynthesis of chlorophyll and generated a kind of senescence [19,20]. Our results are in agreement with finding by Yang et al. [21] who reported that leaves of Potamogeton crispus under Cd stress showed decreased 35,8% chlorophyll a and 26.7% chlorophyll b and chlorophyll a content was found higher than chlorophyll b content. Several report have shown that under Cd stress decrease chlorophyll content in leaf garden grass [22] and almond seedling [23]. Therefore, chlorophyll pigments seem to be one of the main reasons of heavy-metal injury in plants.Figure 1

Bottom Line: Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves.The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry.We found that MDA and antioxidant enzyme (CAT, SOD and APX) contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Cadmium (Cd) is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA) contents, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd.

Results: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased Cd concentration treatments in both roots and leaves. SOD and CAT activities was also increased with the increase Cd concentrations. K, Mn and Mg concentrations were found higher in leaves than roots under Cd stress. In general, increased Cd treatments increased K, Mg, Fe, Ca, Cu and Zn concentration in both roots and leaves. Excessive Cd treatments reduced chlorophyll contents, increased antioxidant enzyme activities and changes in plant nutrition concentrations in both roots and leaves.

Conclusion: The results presented in this work suggested that Cd treatments have negative effect on chlorophyll content and nearly decreased 30% of plant growth in strawberry. Strawberry roots accumulated higher Cd than leaves. We found that MDA and antioxidant enzyme (CAT, SOD and APX) contents may have considered a good indicator in determining Cd tolerance in strawberry plant.

Show MeSH
Related in: MedlinePlus