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Intralymphatic immunotherapy.

Senti G, Kündig TM - World Allergy Organ J (2015)

Bottom Line: Gold Standard allergen-specific immunotherapy is associated with low efficacy because it requires either many subcutaneous injections of allergen or even more numerous sublingual allergen administrations to achieve amelioration of symptoms.Intralymphatic vaccination can maximize immunogenicity and hence efficacy.We and others have demonstrated that as few as three low dose intralymphatic allergen administrations are sufficient to effectively alleviate symptoms.

View Article: PubMed Central - PubMed

Affiliation: Clinical Trials Center, University Hospital Zurich, Raemistrasse 100/MOU2, CH-8091 Zurich, Switzerland.

ABSTRACT
Gold Standard allergen-specific immunotherapy is associated with low efficacy because it requires either many subcutaneous injections of allergen or even more numerous sublingual allergen administrations to achieve amelioration of symptoms. Intralymphatic vaccination can maximize immunogenicity and hence efficacy. We and others have demonstrated that as few as three low dose intralymphatic allergen administrations are sufficient to effectively alleviate symptoms. Results of recent prospective and controlled trials suggest that this strategy may be an effective form of allergen immunotherapy.

No MeSH data available.


Biodistribution after intralymphatic administration. Biodistribution of 99mTc-labelled human IgG after intralymphatic (left abdominal side) and subcutaneous (right abdominal side) injections. Radio tracing was made by gamma-imaging 20 min (left panel) and 25 hours (right panel) after injection. Arrows indicate the site of injection (s.c., subcutaneous, i.l., intralymphatic).
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Fig1: Biodistribution after intralymphatic administration. Biodistribution of 99mTc-labelled human IgG after intralymphatic (left abdominal side) and subcutaneous (right abdominal side) injections. Radio tracing was made by gamma-imaging 20 min (left panel) and 25 hours (right panel) after injection. Arrows indicate the site of injection (s.c., subcutaneous, i.l., intralymphatic).

Mentions: Biodistribution studies in mice revealed that after direct lymph-node injection 100-fold higher antigen doses reached the lymph nodes than after subcutaneous injection in the drained area of a lymph node [45]. Intralymphatic and subcutaneous injections of radiotraced proteins in humans gave similar results. A 99mTc-labeled protein was injected directly into a superficial inguinal lymph node on the right abdominal side. On the left side, the same dose was injected subcutaneously 10 cm above the inguinal lymph nodes. Figure 1 shows that only a small fraction of the subcutaneously administered protein had reached the lymph nodes after 4 hours, and that this fraction had not increased after 25 hours. In contrast, after intralymphatic injection the protein had drained into the deep subcutaneous lymph nodes and already after 20 minutes it was detected in a pelvic lymph node. Intralymphatic injection could efficiently pulse five lymph nodes with the full amount of the protein.Figure 1


Intralymphatic immunotherapy.

Senti G, Kündig TM - World Allergy Organ J (2015)

Biodistribution after intralymphatic administration. Biodistribution of 99mTc-labelled human IgG after intralymphatic (left abdominal side) and subcutaneous (right abdominal side) injections. Radio tracing was made by gamma-imaging 20 min (left panel) and 25 hours (right panel) after injection. Arrows indicate the site of injection (s.c., subcutaneous, i.l., intralymphatic).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352255&req=5

Fig1: Biodistribution after intralymphatic administration. Biodistribution of 99mTc-labelled human IgG after intralymphatic (left abdominal side) and subcutaneous (right abdominal side) injections. Radio tracing was made by gamma-imaging 20 min (left panel) and 25 hours (right panel) after injection. Arrows indicate the site of injection (s.c., subcutaneous, i.l., intralymphatic).
Mentions: Biodistribution studies in mice revealed that after direct lymph-node injection 100-fold higher antigen doses reached the lymph nodes than after subcutaneous injection in the drained area of a lymph node [45]. Intralymphatic and subcutaneous injections of radiotraced proteins in humans gave similar results. A 99mTc-labeled protein was injected directly into a superficial inguinal lymph node on the right abdominal side. On the left side, the same dose was injected subcutaneously 10 cm above the inguinal lymph nodes. Figure 1 shows that only a small fraction of the subcutaneously administered protein had reached the lymph nodes after 4 hours, and that this fraction had not increased after 25 hours. In contrast, after intralymphatic injection the protein had drained into the deep subcutaneous lymph nodes and already after 20 minutes it was detected in a pelvic lymph node. Intralymphatic injection could efficiently pulse five lymph nodes with the full amount of the protein.Figure 1

Bottom Line: Gold Standard allergen-specific immunotherapy is associated with low efficacy because it requires either many subcutaneous injections of allergen or even more numerous sublingual allergen administrations to achieve amelioration of symptoms.Intralymphatic vaccination can maximize immunogenicity and hence efficacy.We and others have demonstrated that as few as three low dose intralymphatic allergen administrations are sufficient to effectively alleviate symptoms.

View Article: PubMed Central - PubMed

Affiliation: Clinical Trials Center, University Hospital Zurich, Raemistrasse 100/MOU2, CH-8091 Zurich, Switzerland.

ABSTRACT
Gold Standard allergen-specific immunotherapy is associated with low efficacy because it requires either many subcutaneous injections of allergen or even more numerous sublingual allergen administrations to achieve amelioration of symptoms. Intralymphatic vaccination can maximize immunogenicity and hence efficacy. We and others have demonstrated that as few as three low dose intralymphatic allergen administrations are sufficient to effectively alleviate symptoms. Results of recent prospective and controlled trials suggest that this strategy may be an effective form of allergen immunotherapy.

No MeSH data available.