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Vaccination with recombinant paramyosin against the bovine lungworm Dictyocaulus viviparus considerably reduces worm burden and larvae shedding.

Strube C, Haake C, Sager H, Schorderet Weber S, Kaminsky R, Buschbaum S, Joekel D, Schicht S, Kremmer E, Korrell J, Schnieder T, von Samson-Himmelstjerna G - Parasit Vectors (2015)

Bottom Line: As a recombinant subunit vaccine would overcome these disadvantages, the parasite's muscle protein paramyosin (PMY) was tested as a recombinant vaccine antigen.Immunohistochemical staining revealed that PMY is part of the bovine lungworm's pharyngeal and body wall muscles.Even though vaccination with the attenuated live vaccine was with 94% (geometric mean: 95%) reduction in larvae shedding and 93% (geometric mean: 94%) reduction in worm burden superior to rPMY vaccination, results using the latter are promising and show the potential for further development of a recombinant PMY-based vaccine against the bovine lungworm.

View Article: PubMed Central - PubMed

Affiliation: Institute for Parasitology, University of Veterinary Medicine Hannover, Hanover, Germany. christina.strube@tiho-hannover.de.

ABSTRACT

Background: The lungworm Dictyocaulus viviparus, causing parasitic bronchitis in cattle, induces a temporary protective immunity that prevents clinical disease. A radiation-attenuated larvae based vaccine is commercially available in a few European countries, but has the disadvantages of a live vaccine. As a recombinant subunit vaccine would overcome these disadvantages, the parasite's muscle protein paramyosin (PMY) was tested as a recombinant vaccine antigen.

Methods: D. viviparus-PMY was recombinantly expressed in Escherichia coli as a glutathione-S-transferase (GST)-fused protein. Emulsified in adjuvant Saponin Quil A, the protein was given intramuscularly into calves. Two independent recombinant PMY (rPMY) vaccination trials with negative control groups (first trial: adjuvant only; second trial: non-fused GST) as well as an additional positive control group in the second trial, using the Bovilis Dictol live vaccine to verify vaccination results, were performed. To determine the vaccination success, shedding of larvae as well as worm burden and worm sizes were analyzed. Additionally, ELISA-based determination of development of immunglobulins IgM, IgA, IgE, IgG as well as the subclasses IgG1 and IgG2 was performed. To analyze PMY localization in the bovine lungworm, immunohistochemical staining of adult worms was carried out.

Results: Immunohistochemical staining revealed that PMY is part of the bovine lungworm's pharyngeal and body wall muscles. Vaccination with rPMY resulted in 47% [geometric mean: 67%] and 57% (geometric mean: 71%) reduction of larvae shedding in the first and second vaccination trial, respectively. Worm burden was reduced by 54% (geometric mean: 86%) and 31% (geometric mean: 68%), respectively, and worms of rPMY-vaccinated cattle were significantly shorter in both trials. Furthermore, ELISAs showed a clear antibody response towards rPMY with exception of IgE for which titers could not be detected. After challenge infection, rPMY antibodies were only exceptionally elevated among study animals indicating PMY to be a hidden antigen.

Conclusions: Even though vaccination with the attenuated live vaccine was with 94% (geometric mean: 95%) reduction in larvae shedding and 93% (geometric mean: 94%) reduction in worm burden superior to rPMY vaccination, results using the latter are promising and show the potential for further development of a recombinant PMY-based vaccine against the bovine lungworm.

No MeSH data available.


Related in: MedlinePlus

Anti-rPMY-antibody development during the second vaccination trial. Optical density (OD) values over time are for immunoglobulin (Ig) classes IgG, IgG1, IgG2, IgM, IgA, IgM and IgE for the individual study animals. The graph legend lists animal IDs of each group.
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Fig2: Anti-rPMY-antibody development during the second vaccination trial. Optical density (OD) values over time are for immunoglobulin (Ig) classes IgG, IgG1, IgG2, IgM, IgA, IgM and IgE for the individual study animals. The graph legend lists animal IDs of each group.

Mentions: After challenge infection, no increase in antibody intensity was detected within any of the groups, except of animal no. 808 (adjuvant only-control, trial 1), which showed a distinct IgG and IgG1 increase and a slight IgA increase. Furthermore, animal no. 805 (adjuvant only-control, trial 1) showed increased IgM levels. Sera of the Dictol group did not show any antibody response to rPMY. Determined anti-PMY-antibody curves are shown in Figure 1 (first vaccination trial) and Figure 2 (second vaccination trial).Figure 1


Vaccination with recombinant paramyosin against the bovine lungworm Dictyocaulus viviparus considerably reduces worm burden and larvae shedding.

Strube C, Haake C, Sager H, Schorderet Weber S, Kaminsky R, Buschbaum S, Joekel D, Schicht S, Kremmer E, Korrell J, Schnieder T, von Samson-Himmelstjerna G - Parasit Vectors (2015)

Anti-rPMY-antibody development during the second vaccination trial. Optical density (OD) values over time are for immunoglobulin (Ig) classes IgG, IgG1, IgG2, IgM, IgA, IgM and IgE for the individual study animals. The graph legend lists animal IDs of each group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4352246&req=5

Fig2: Anti-rPMY-antibody development during the second vaccination trial. Optical density (OD) values over time are for immunoglobulin (Ig) classes IgG, IgG1, IgG2, IgM, IgA, IgM and IgE for the individual study animals. The graph legend lists animal IDs of each group.
Mentions: After challenge infection, no increase in antibody intensity was detected within any of the groups, except of animal no. 808 (adjuvant only-control, trial 1), which showed a distinct IgG and IgG1 increase and a slight IgA increase. Furthermore, animal no. 805 (adjuvant only-control, trial 1) showed increased IgM levels. Sera of the Dictol group did not show any antibody response to rPMY. Determined anti-PMY-antibody curves are shown in Figure 1 (first vaccination trial) and Figure 2 (second vaccination trial).Figure 1

Bottom Line: As a recombinant subunit vaccine would overcome these disadvantages, the parasite's muscle protein paramyosin (PMY) was tested as a recombinant vaccine antigen.Immunohistochemical staining revealed that PMY is part of the bovine lungworm's pharyngeal and body wall muscles.Even though vaccination with the attenuated live vaccine was with 94% (geometric mean: 95%) reduction in larvae shedding and 93% (geometric mean: 94%) reduction in worm burden superior to rPMY vaccination, results using the latter are promising and show the potential for further development of a recombinant PMY-based vaccine against the bovine lungworm.

View Article: PubMed Central - PubMed

Affiliation: Institute for Parasitology, University of Veterinary Medicine Hannover, Hanover, Germany. christina.strube@tiho-hannover.de.

ABSTRACT

Background: The lungworm Dictyocaulus viviparus, causing parasitic bronchitis in cattle, induces a temporary protective immunity that prevents clinical disease. A radiation-attenuated larvae based vaccine is commercially available in a few European countries, but has the disadvantages of a live vaccine. As a recombinant subunit vaccine would overcome these disadvantages, the parasite's muscle protein paramyosin (PMY) was tested as a recombinant vaccine antigen.

Methods: D. viviparus-PMY was recombinantly expressed in Escherichia coli as a glutathione-S-transferase (GST)-fused protein. Emulsified in adjuvant Saponin Quil A, the protein was given intramuscularly into calves. Two independent recombinant PMY (rPMY) vaccination trials with negative control groups (first trial: adjuvant only; second trial: non-fused GST) as well as an additional positive control group in the second trial, using the Bovilis Dictol live vaccine to verify vaccination results, were performed. To determine the vaccination success, shedding of larvae as well as worm burden and worm sizes were analyzed. Additionally, ELISA-based determination of development of immunglobulins IgM, IgA, IgE, IgG as well as the subclasses IgG1 and IgG2 was performed. To analyze PMY localization in the bovine lungworm, immunohistochemical staining of adult worms was carried out.

Results: Immunohistochemical staining revealed that PMY is part of the bovine lungworm's pharyngeal and body wall muscles. Vaccination with rPMY resulted in 47% [geometric mean: 67%] and 57% (geometric mean: 71%) reduction of larvae shedding in the first and second vaccination trial, respectively. Worm burden was reduced by 54% (geometric mean: 86%) and 31% (geometric mean: 68%), respectively, and worms of rPMY-vaccinated cattle were significantly shorter in both trials. Furthermore, ELISAs showed a clear antibody response towards rPMY with exception of IgE for which titers could not be detected. After challenge infection, rPMY antibodies were only exceptionally elevated among study animals indicating PMY to be a hidden antigen.

Conclusions: Even though vaccination with the attenuated live vaccine was with 94% (geometric mean: 95%) reduction in larvae shedding and 93% (geometric mean: 94%) reduction in worm burden superior to rPMY vaccination, results using the latter are promising and show the potential for further development of a recombinant PMY-based vaccine against the bovine lungworm.

No MeSH data available.


Related in: MedlinePlus