Limits...
Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

Show MeSH

Related in: MedlinePlus

The viability of the HDFa cells after a 1-h incubation in the presence of 1 mM H2O2 and different concentrations of investigated complex compounds. K denotes cells not treated with H2O2 nor complexes (control cells). Values represent means of three different experiments (run in triplicate) ± SD
© Copyright Policy - OpenAccess
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4352194&req=5

Fig5: The viability of the HDFa cells after a 1-h incubation in the presence of 1 mM H2O2 and different concentrations of investigated complex compounds. K denotes cells not treated with H2O2 nor complexes (control cells). Values represent means of three different experiments (run in triplicate) ± SD

Mentions: Screening of cytoprotective properties of VO(IV), Ni(II) and Co(II) complexes has shown that Co(ODA) exhibits the highest dose- and time-dependent protective effect against the oxidative damage. The results of the MTT test (Figs. 5 and 6) revealed that the viability of human fibroblasts treated with 1 mM H2O2 together with the complex for 1 h increases in proportion to the amount of Co(ODA) in the system (Fig. 5). The viability of cells increases up to ca. 100 % in the case of treatment with 500 μM Co(ODA) and is comparable to the untreated cells (Fig. 5). The ability of cells, first exposed to H2O2 and Co(ODA), to grow and proliferate at similar levels to untreated control cells after an additional 48-h incubation in the normal growth medium, confirms the cytoprotective activity of this complex against the oxidative damage (Fig. 6). Interestingly, also VO(ODA), to some extent, exhibits the cytoprotective activity against the oxidative damage to cells at the short, 1-h exposure time (Fig. 5), but due to its strong cytotoxic activity, VO(ODA) itself induces the cell death and the effect is visible after the additional 48-h incubation of cells in the growth medium (Fig. 6). A different situation is seen for the Ni(ODA) complex. It has no influence on the viability of cells, regardless of the dose used together with H2O2 (Figs. 5 and 6).Fig. 5


Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

The viability of the HDFa cells after a 1-h incubation in the presence of 1 mM H2O2 and different concentrations of investigated complex compounds. K denotes cells not treated with H2O2 nor complexes (control cells). Values represent means of three different experiments (run in triplicate) ± SD
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352194&req=5

Fig5: The viability of the HDFa cells after a 1-h incubation in the presence of 1 mM H2O2 and different concentrations of investigated complex compounds. K denotes cells not treated with H2O2 nor complexes (control cells). Values represent means of three different experiments (run in triplicate) ± SD
Mentions: Screening of cytoprotective properties of VO(IV), Ni(II) and Co(II) complexes has shown that Co(ODA) exhibits the highest dose- and time-dependent protective effect against the oxidative damage. The results of the MTT test (Figs. 5 and 6) revealed that the viability of human fibroblasts treated with 1 mM H2O2 together with the complex for 1 h increases in proportion to the amount of Co(ODA) in the system (Fig. 5). The viability of cells increases up to ca. 100 % in the case of treatment with 500 μM Co(ODA) and is comparable to the untreated cells (Fig. 5). The ability of cells, first exposed to H2O2 and Co(ODA), to grow and proliferate at similar levels to untreated control cells after an additional 48-h incubation in the normal growth medium, confirms the cytoprotective activity of this complex against the oxidative damage (Fig. 6). Interestingly, also VO(ODA), to some extent, exhibits the cytoprotective activity against the oxidative damage to cells at the short, 1-h exposure time (Fig. 5), but due to its strong cytotoxic activity, VO(ODA) itself induces the cell death and the effect is visible after the additional 48-h incubation of cells in the growth medium (Fig. 6). A different situation is seen for the Ni(ODA) complex. It has no influence on the viability of cells, regardless of the dose used together with H2O2 (Figs. 5 and 6).Fig. 5

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

Show MeSH
Related in: MedlinePlus