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Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

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The influence of VO(ODA), Ni(ODA) and Co(ODA) on the relative proliferation of HDFa cells after 6 days of exposure to investigated complex compounds (based on 24-h BrdU incorporation by actively dividing cells). Values represent means of four different experiments (run in duplicate) ± SD
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Fig4: The influence of VO(ODA), Ni(ODA) and Co(ODA) on the relative proliferation of HDFa cells after 6 days of exposure to investigated complex compounds (based on 24-h BrdU incorporation by actively dividing cells). Values represent means of four different experiments (run in duplicate) ± SD

Mentions: The dose-dependent (in the concentration range of 1–1000 μM) effect of the complexes on the HDFa cell proliferation was investigated by the measurement of BrdU (5-bromo-2′-deoxyuridine) incorporation by actively dividing cells after 6 days of culture in the presence of different concentrations of complexes (Fig. 4). Based on the obtained data, inhibitory concentrations (IC25, IC50 and IC75) for each compound were calculated and they are summarized in Table 6. The highest antiproliferative activity was found for oxovanadium(IV) complex (IC50 = 25 μM). At higher concentrations, the synergy effect, connected with the increase of the VO(ODA) cytotoxicity with the exposure time, on the antiproliferative activity was observed. The Co(II) and Ni(II) complexes exhibit the antiproliferative activity comparable to each other, and they cause the 50 % inhibition of cell proliferation at ca. 200 μM.Fig. 4


Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

The influence of VO(ODA), Ni(ODA) and Co(ODA) on the relative proliferation of HDFa cells after 6 days of exposure to investigated complex compounds (based on 24-h BrdU incorporation by actively dividing cells). Values represent means of four different experiments (run in duplicate) ± SD
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352194&req=5

Fig4: The influence of VO(ODA), Ni(ODA) and Co(ODA) on the relative proliferation of HDFa cells after 6 days of exposure to investigated complex compounds (based on 24-h BrdU incorporation by actively dividing cells). Values represent means of four different experiments (run in duplicate) ± SD
Mentions: The dose-dependent (in the concentration range of 1–1000 μM) effect of the complexes on the HDFa cell proliferation was investigated by the measurement of BrdU (5-bromo-2′-deoxyuridine) incorporation by actively dividing cells after 6 days of culture in the presence of different concentrations of complexes (Fig. 4). Based on the obtained data, inhibitory concentrations (IC25, IC50 and IC75) for each compound were calculated and they are summarized in Table 6. The highest antiproliferative activity was found for oxovanadium(IV) complex (IC50 = 25 μM). At higher concentrations, the synergy effect, connected with the increase of the VO(ODA) cytotoxicity with the exposure time, on the antiproliferative activity was observed. The Co(II) and Ni(II) complexes exhibit the antiproliferative activity comparable to each other, and they cause the 50 % inhibition of cell proliferation at ca. 200 μM.Fig. 4

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

Show MeSH
Related in: MedlinePlus