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Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

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The superoxide free radicals scavenging activity of the complexes
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Fig3: The superoxide free radicals scavenging activity of the complexes

Mentions: The plots of the percentage of inhibition versus complex concentrations are presented in Fig. 3. It has been found that all the complexes exhibit the activity towards scavenging of superoxide anions. The results obtained from the NBT assay are in agreement with those obtained earlier by using the CV method [16]. Both methods have proved that the complexes are capable to scavenge superoxide free radicals. A linear relation was obtained for the cobalt(II) complex (Fig. 3). For the oxovanadium(IV) and nickel(II) complexes, the relationships were found to be non-linear. In that case, the best fitting curves were obtained according to the exponential function described by the equation: y = y0 + A∙e(x/t), where y denoted the percentage of inhibition and x denoted the complex concentration. The differences in the relationships (linear/non-linear) showed in Fig. 3 suggest a different mechanism of scavenging superoxide free radicals. However, the mechanism of the reaction of the complexes with O2− is not easy to elucidate.Fig. 3


Physicochemical and biological properties of oxovanadium(IV), cobalt(II) and nickel(II) complexes with oxydiacetate anions.

Wyrzykowski D, Kloska A, Pranczk J, Szczepańska A, Tesmar A, Jacewicz D, Pilarski B, Chmurzyński L - Biol Trace Elem Res (2014)

The superoxide free radicals scavenging activity of the complexes
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4352194&req=5

Fig3: The superoxide free radicals scavenging activity of the complexes
Mentions: The plots of the percentage of inhibition versus complex concentrations are presented in Fig. 3. It has been found that all the complexes exhibit the activity towards scavenging of superoxide anions. The results obtained from the NBT assay are in agreement with those obtained earlier by using the CV method [16]. Both methods have proved that the complexes are capable to scavenge superoxide free radicals. A linear relation was obtained for the cobalt(II) complex (Fig. 3). For the oxovanadium(IV) and nickel(II) complexes, the relationships were found to be non-linear. In that case, the best fitting curves were obtained according to the exponential function described by the equation: y = y0 + A∙e(x/t), where y denoted the percentage of inhibition and x denoted the complex concentration. The differences in the relationships (linear/non-linear) showed in Fig. 3 suggest a different mechanism of scavenging superoxide free radicals. However, the mechanism of the reaction of the complexes with O2− is not easy to elucidate.Fig. 3

Bottom Line: The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated.Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay.The relationship between physicochemical and biological properties of the complexes was discussed.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdańsk, Poland, dariusz.wyrzykowski@ug.edu.pl.

ABSTRACT
The potentiometric and conductometric titration methods have been used to characterize the stability of series of VO(IV)-, Co(II)- and Ni(II)-oxydiacetato complexes in DMSO-water solutions containing 0-50 % (v/v) DMSO. The influence of DMSO as a co-solvent on the stability of the complexes as well as the oxydiacetic acid was evaluated. Furthermore, the reactivity of the complexes towards superoxide free radicals was assessed by employing the nitro blue tetrazolium (NBT) assay. The biological properties of the complexes were investigated in relation to their cytoprotective activity against the oxidative damage generated exogenously by using hydrogen peroxide in the Human Dermal Fibroblasts adult (HDFa) cell line as well as to their antimicrobial activity against the bacteria (Bacillus subtilis, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis). The relationship between physicochemical and biological properties of the complexes was discussed.

Show MeSH
Related in: MedlinePlus