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Intermittent fasting results in tissue-specific changes in bioenergetics and redox state.

Chausse B, Vieira-Lara MA, Sanchez AB, Medeiros MH, Kowaltowski AJ - PLoS ONE (2015)

Bottom Line: Although the consequences of CR are well studied, the effects of IF on redox status are not.No difference in mitochondrial bioenergetics or redox homeostasis was observed in skeletal muscles of IF animals.Overall, IF affects redox balance in a tissue-specific manner, leading to redox imbalance in the liver and brain and protection against oxidative damage in the heart.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.

ABSTRACT
Intermittent fasting (IF) is a dietary intervention often used as an alternative to caloric restriction (CR) and characterized by 24 hour cycles alternating ad libitum feeding and fasting. Although the consequences of CR are well studied, the effects of IF on redox status are not. Here, we address the effects of IF on redox state markers in different tissues in order to uncover how changes in feeding frequency alter redox balance in rats. IF rats displayed lower body mass due to decreased energy conversion efficiency. Livers in IF rats presented increased mitochondrial respiratory capacity and enhanced levels of protein carbonyls. Surprisingly, IF animals also presented an increase in oxidative damage in the brain that was not related to changes in mitochondrial bioenergetics. Conversely, IF promoted a substantial protection against oxidative damage in the heart. No difference in mitochondrial bioenergetics or redox homeostasis was observed in skeletal muscles of IF animals. Overall, IF affects redox balance in a tissue-specific manner, leading to redox imbalance in the liver and brain and protection against oxidative damage in the heart.

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Intermittent fasting promotes lower body mass related to a mild reduction in caloric intake and lower energy conversion efficiency.(A) Average body weight per cage in (●) AL and (☐) IF animals after one month of treatment. (B) Cumulative food intake over one month of treatment. (C) Energy conversion efficiency. Data represent averages ± SEM and were compared using t tests (n = 7 cages). ** p<0.01 vs AL. AL indicates ad libitum feeding, IF indicates intermittent fasting.
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pone.0120413.g001: Intermittent fasting promotes lower body mass related to a mild reduction in caloric intake and lower energy conversion efficiency.(A) Average body weight per cage in (●) AL and (☐) IF animals after one month of treatment. (B) Cumulative food intake over one month of treatment. (C) Energy conversion efficiency. Data represent averages ± SEM and were compared using t tests (n = 7 cages). ** p<0.01 vs AL. AL indicates ad libitum feeding, IF indicates intermittent fasting.

Mentions: IF diets have been extensively shown to reduce body mass [6, 7, 9]. Indeed, we observed that, after a month, IF rats presented lower body weights than AL control rats (Fig. 1A). The decrease in body weight was a reflection of two additive effects of the diet: a 14% reduction in food consumption (Fig. 1B) and a 40% decrease in energy conversion efficiency (Fig. 1C), or the amount of food ingested that results in weight gain. Similar decreases in energy conversion efficiency in IF have been reported previously and may be related to changes in hypothalamic energy metabolism control [25].


Intermittent fasting results in tissue-specific changes in bioenergetics and redox state.

Chausse B, Vieira-Lara MA, Sanchez AB, Medeiros MH, Kowaltowski AJ - PLoS ONE (2015)

Intermittent fasting promotes lower body mass related to a mild reduction in caloric intake and lower energy conversion efficiency.(A) Average body weight per cage in (●) AL and (☐) IF animals after one month of treatment. (B) Cumulative food intake over one month of treatment. (C) Energy conversion efficiency. Data represent averages ± SEM and were compared using t tests (n = 7 cages). ** p<0.01 vs AL. AL indicates ad libitum feeding, IF indicates intermittent fasting.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352038&req=5

pone.0120413.g001: Intermittent fasting promotes lower body mass related to a mild reduction in caloric intake and lower energy conversion efficiency.(A) Average body weight per cage in (●) AL and (☐) IF animals after one month of treatment. (B) Cumulative food intake over one month of treatment. (C) Energy conversion efficiency. Data represent averages ± SEM and were compared using t tests (n = 7 cages). ** p<0.01 vs AL. AL indicates ad libitum feeding, IF indicates intermittent fasting.
Mentions: IF diets have been extensively shown to reduce body mass [6, 7, 9]. Indeed, we observed that, after a month, IF rats presented lower body weights than AL control rats (Fig. 1A). The decrease in body weight was a reflection of two additive effects of the diet: a 14% reduction in food consumption (Fig. 1B) and a 40% decrease in energy conversion efficiency (Fig. 1C), or the amount of food ingested that results in weight gain. Similar decreases in energy conversion efficiency in IF have been reported previously and may be related to changes in hypothalamic energy metabolism control [25].

Bottom Line: Although the consequences of CR are well studied, the effects of IF on redox status are not.No difference in mitochondrial bioenergetics or redox homeostasis was observed in skeletal muscles of IF animals.Overall, IF affects redox balance in a tissue-specific manner, leading to redox imbalance in the liver and brain and protection against oxidative damage in the heart.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil.

ABSTRACT
Intermittent fasting (IF) is a dietary intervention often used as an alternative to caloric restriction (CR) and characterized by 24 hour cycles alternating ad libitum feeding and fasting. Although the consequences of CR are well studied, the effects of IF on redox status are not. Here, we address the effects of IF on redox state markers in different tissues in order to uncover how changes in feeding frequency alter redox balance in rats. IF rats displayed lower body mass due to decreased energy conversion efficiency. Livers in IF rats presented increased mitochondrial respiratory capacity and enhanced levels of protein carbonyls. Surprisingly, IF animals also presented an increase in oxidative damage in the brain that was not related to changes in mitochondrial bioenergetics. Conversely, IF promoted a substantial protection against oxidative damage in the heart. No difference in mitochondrial bioenergetics or redox homeostasis was observed in skeletal muscles of IF animals. Overall, IF affects redox balance in a tissue-specific manner, leading to redox imbalance in the liver and brain and protection against oxidative damage in the heart.

Show MeSH
Related in: MedlinePlus