Limits...
Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

Show MeSH

Related in: MedlinePlus

Effect of instillation of silver nanoparticles capped with citrate (cit) or polyvinlypyrrolidone (pvp) on BAL levels of protein, malonaldehyde (MDA) and eosinophil cationic protein (ECP), and on lung tissue ECP.Data is expressed as mean ± SD, n = 5–6 for each. *P<0.05, **P<0.001 and P<0.0001 compared to water control (C).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4352037&req=5

pone.0119726.g004: Effect of instillation of silver nanoparticles capped with citrate (cit) or polyvinlypyrrolidone (pvp) on BAL levels of protein, malonaldehyde (MDA) and eosinophil cationic protein (ECP), and on lung tissue ECP.Data is expressed as mean ± SD, n = 5–6 for each. *P<0.05, **P<0.001 and P<0.0001 compared to water control (C).

Mentions: In BN rats, total BAL protein was increased by 3–4 fold at 24 hours, with a greater increase observed for Ag20citrate and Ag20pvp, that also persisted at day 7, but levels returned towards baseline at 21 days (Fig. 4A). In SD rats, total protein levels only transiently increased by 2-fold at day 7 for both 20 nm particles (Fig. 4D).


Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Effect of instillation of silver nanoparticles capped with citrate (cit) or polyvinlypyrrolidone (pvp) on BAL levels of protein, malonaldehyde (MDA) and eosinophil cationic protein (ECP), and on lung tissue ECP.Data is expressed as mean ± SD, n = 5–6 for each. *P<0.05, **P<0.001 and P<0.0001 compared to water control (C).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352037&req=5

pone.0119726.g004: Effect of instillation of silver nanoparticles capped with citrate (cit) or polyvinlypyrrolidone (pvp) on BAL levels of protein, malonaldehyde (MDA) and eosinophil cationic protein (ECP), and on lung tissue ECP.Data is expressed as mean ± SD, n = 5–6 for each. *P<0.05, **P<0.001 and P<0.0001 compared to water control (C).
Mentions: In BN rats, total BAL protein was increased by 3–4 fold at 24 hours, with a greater increase observed for Ag20citrate and Ag20pvp, that also persisted at day 7, but levels returned towards baseline at 21 days (Fig. 4A). In SD rats, total protein levels only transiently increased by 2-fold at day 7 for both 20 nm particles (Fig. 4D).

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

Show MeSH
Related in: MedlinePlus