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Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

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Representative haematoxylin- and carbo-chromotrope-stained lung sections from Brown-Norway (BN) rat lungs exposed to water only (Control) or to 20nm and 110 nm citrate- or pvp-capped silver nanoparticles (Panels A-E), and from Sprague-Dawley (SD) rats exposed to water only (Control) or to silver 20nm citrate- or pvp-capped nanoparticles (Panels F-H) at 24 hours after instillation.Compared to controls, inflammatory responses with neutrophils (arrow heads) are seen in both BN and SD rats after nanoparticle instillation. BN rats show the a predominance of eosinophils, while in SD rats, the inflammation is predominantly neutrophilic (Scale bar on Panel G is 10 μm, as used throughout). Panel I shows the distribution of inflammatory scores in these experiments with median bars shown for each experimental group. C is control rats instilled with water alone. *p<0.05; **p<0.01; ***p<0.001 compared to C; #p<0.05 compared to appropriate Brown-Norway group.
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pone.0119726.g003: Representative haematoxylin- and carbo-chromotrope-stained lung sections from Brown-Norway (BN) rat lungs exposed to water only (Control) or to 20nm and 110 nm citrate- or pvp-capped silver nanoparticles (Panels A-E), and from Sprague-Dawley (SD) rats exposed to water only (Control) or to silver 20nm citrate- or pvp-capped nanoparticles (Panels F-H) at 24 hours after instillation.Compared to controls, inflammatory responses with neutrophils (arrow heads) are seen in both BN and SD rats after nanoparticle instillation. BN rats show the a predominance of eosinophils, while in SD rats, the inflammation is predominantly neutrophilic (Scale bar on Panel G is 10 μm, as used throughout). Panel I shows the distribution of inflammatory scores in these experiments with median bars shown for each experimental group. C is control rats instilled with water alone. *p<0.05; **p<0.01; ***p<0.001 compared to C; #p<0.05 compared to appropriate Brown-Norway group.

Mentions: In the lungs, the inflammatory scores increased in both BN and SD rats at 24 hours but to a lesser extent in the SD than in the BN rat (Fig. 3I). The inflammatory response was predominantly neutrophilic, but the additional eosinophilic response was more prominent in the BN rat (Fig. 3A to 3H). These lung neutrophilic inflammation were similar to that observed in BAL fluid.


Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Representative haematoxylin- and carbo-chromotrope-stained lung sections from Brown-Norway (BN) rat lungs exposed to water only (Control) or to 20nm and 110 nm citrate- or pvp-capped silver nanoparticles (Panels A-E), and from Sprague-Dawley (SD) rats exposed to water only (Control) or to silver 20nm citrate- or pvp-capped nanoparticles (Panels F-H) at 24 hours after instillation.Compared to controls, inflammatory responses with neutrophils (arrow heads) are seen in both BN and SD rats after nanoparticle instillation. BN rats show the a predominance of eosinophils, while in SD rats, the inflammation is predominantly neutrophilic (Scale bar on Panel G is 10 μm, as used throughout). Panel I shows the distribution of inflammatory scores in these experiments with median bars shown for each experimental group. C is control rats instilled with water alone. *p<0.05; **p<0.01; ***p<0.001 compared to C; #p<0.05 compared to appropriate Brown-Norway group.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352037&req=5

pone.0119726.g003: Representative haematoxylin- and carbo-chromotrope-stained lung sections from Brown-Norway (BN) rat lungs exposed to water only (Control) or to 20nm and 110 nm citrate- or pvp-capped silver nanoparticles (Panels A-E), and from Sprague-Dawley (SD) rats exposed to water only (Control) or to silver 20nm citrate- or pvp-capped nanoparticles (Panels F-H) at 24 hours after instillation.Compared to controls, inflammatory responses with neutrophils (arrow heads) are seen in both BN and SD rats after nanoparticle instillation. BN rats show the a predominance of eosinophils, while in SD rats, the inflammation is predominantly neutrophilic (Scale bar on Panel G is 10 μm, as used throughout). Panel I shows the distribution of inflammatory scores in these experiments with median bars shown for each experimental group. C is control rats instilled with water alone. *p<0.05; **p<0.01; ***p<0.001 compared to C; #p<0.05 compared to appropriate Brown-Norway group.
Mentions: In the lungs, the inflammatory scores increased in both BN and SD rats at 24 hours but to a lesser extent in the SD than in the BN rat (Fig. 3I). The inflammatory response was predominantly neutrophilic, but the additional eosinophilic response was more prominent in the BN rat (Fig. 3A to 3H). These lung neutrophilic inflammation were similar to that observed in BAL fluid.

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

Show MeSH
Related in: MedlinePlus