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Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

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Characterisation of silver nanoparticle size, stability, composition and solubility.Panel A: TEM images of 20 nm and 110 nm silver nanoparticles either PVP- or citrate-capped following a 24 hour incubation in water (pH7, 37°C) in the absence (-) and presence (+) of dipalmitoylphosphatidylcholine (DPPC). Panel B: EDS spectra of (i) Ag20PVP without DPPC (First circle in upper part of Panel A) and (ii) Ag20PVP with DPPC (First circle in lower part of Panel A). Panel C: Time-dependent dissolution of 20 nm silver nanoparticle citrate- or PVP-capped suspensions (25 μg/mL) in water (pH7, 37°C), or in water (pH7, 37°C) plus DPPC. Ion concentrations are shown as μg/mL as determined by ICP-MS. Data shown as mean ± SD of three incubations.
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pone.0119726.g001: Characterisation of silver nanoparticle size, stability, composition and solubility.Panel A: TEM images of 20 nm and 110 nm silver nanoparticles either PVP- or citrate-capped following a 24 hour incubation in water (pH7, 37°C) in the absence (-) and presence (+) of dipalmitoylphosphatidylcholine (DPPC). Panel B: EDS spectra of (i) Ag20PVP without DPPC (First circle in upper part of Panel A) and (ii) Ag20PVP with DPPC (First circle in lower part of Panel A). Panel C: Time-dependent dissolution of 20 nm silver nanoparticle citrate- or PVP-capped suspensions (25 μg/mL) in water (pH7, 37°C), or in water (pH7, 37°C) plus DPPC. Ion concentrations are shown as μg/mL as determined by ICP-MS. Data shown as mean ± SD of three incubations.

Mentions: TEM images confirmed that the AgNPs tended to agglomerate following incubation in water for 24 hours at 37°C, pH 7; denser agglomerates were observed for the 20nm compared with the 110nm particles (Fig. 1A). Addition of dipalmitoylphosphatidylcholine (DPPC) reduced agglomeration for all AgNPs, with greatest effects on the 110nm particles. EDS spectra, taken under identical acquisition conditions, confirmed a higher local concentration of silver from within the aggregates before and after the addition of DPPC (Fig. 1B).


Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain.

Seiffert J, Hussain F, Wiegman C, Li F, Bey L, Baker W, Porter A, Ryan MP, Chang Y, Gow A, Zhang J, Zhu J, Tetley TD, Chung KF - PLoS ONE (2015)

Characterisation of silver nanoparticle size, stability, composition and solubility.Panel A: TEM images of 20 nm and 110 nm silver nanoparticles either PVP- or citrate-capped following a 24 hour incubation in water (pH7, 37°C) in the absence (-) and presence (+) of dipalmitoylphosphatidylcholine (DPPC). Panel B: EDS spectra of (i) Ag20PVP without DPPC (First circle in upper part of Panel A) and (ii) Ag20PVP with DPPC (First circle in lower part of Panel A). Panel C: Time-dependent dissolution of 20 nm silver nanoparticle citrate- or PVP-capped suspensions (25 μg/mL) in water (pH7, 37°C), or in water (pH7, 37°C) plus DPPC. Ion concentrations are shown as μg/mL as determined by ICP-MS. Data shown as mean ± SD of three incubations.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352037&req=5

pone.0119726.g001: Characterisation of silver nanoparticle size, stability, composition and solubility.Panel A: TEM images of 20 nm and 110 nm silver nanoparticles either PVP- or citrate-capped following a 24 hour incubation in water (pH7, 37°C) in the absence (-) and presence (+) of dipalmitoylphosphatidylcholine (DPPC). Panel B: EDS spectra of (i) Ag20PVP without DPPC (First circle in upper part of Panel A) and (ii) Ag20PVP with DPPC (First circle in lower part of Panel A). Panel C: Time-dependent dissolution of 20 nm silver nanoparticle citrate- or PVP-capped suspensions (25 μg/mL) in water (pH7, 37°C), or in water (pH7, 37°C) plus DPPC. Ion concentrations are shown as μg/mL as determined by ICP-MS. Data shown as mean ± SD of three incubations.
Mentions: TEM images confirmed that the AgNPs tended to agglomerate following incubation in water for 24 hours at 37°C, pH 7; denser agglomerates were observed for the 20nm compared with the 110nm particles (Fig. 1A). Addition of dipalmitoylphosphatidylcholine (DPPC) reduced agglomeration for all AgNPs, with greatest effects on the 110nm particles. EDS spectra, taken under identical acquisition conditions, confirmed a higher local concentration of silver from within the aggregates before and after the addition of DPPC (Fig. 1B).

Bottom Line: Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7.The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs.AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

View Article: PubMed Central - PubMed

Affiliation: Airways Disease, National Heart & Lung Institute, Imperial College, London, United Kingdom.

ABSTRACT
Particle size and surface chemistry are potential determinants of silver nanoparticle (AgNP) respiratory toxicity that may also depend on the lung inflammatory state. We compared the effects of intratracheally-administered AgNPs (20 nm and 110 nm; polyvinylpyrrolidone (PVP) and citrate-capped; 0.1 mg/Kg) in Brown-Norway (BN) and Sprague-Dawley (SD) rats. In BN rats, there was both a neutrophilic and eosinophilic response, while in SD rats, there was a neutrophilic response at day 1, greatest for the 20 nm citrate-capped AgNPs. Eosinophilic cationic protein was increased in bronchoalveolar lavage (BAL) in BN and SD rats on day 1. BAL protein and malondialdehyde levels were increased in BN rats at 1 and 7 days, and BAL KC, CCL11 and IL-13 levels at day 1, with increased expression of CCL11 in lung tissue. Pulmonary resistance increased and compliance decreased at day 1, with persistence at day 7. The 20 nm, but not the 110 nm, AgNPs increased bronchial hyperresponsiveness on day 1, which continued at day 7 for the citrate-capped AgNPs only. The 20 nm versus the 110 nm size were more proinflammatory in terms of neutrophil influx, but there was little difference between the citrate-capped versus the PVP-capped AgNPs. AgNPs can induce pulmonary eosinophilic and neutrophilic inflammation with bronchial hyperresponsiveness, features characteristic of asthma.

Show MeSH
Related in: MedlinePlus