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Chemoprotective effect of taurine on potassium bromate-induced DNA damage, DNA-protein cross-linking and oxidative stress in rat intestine.

Ahmad MK, Khan AA, Ali SN, Mahmood R - PLoS ONE (2015)

Bottom Line: Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered.Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect.Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT
Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. It induces multiple organ toxicity in humans and experimental animals and is a probable human carcinogen. The present study reports the protective effect of dietary antioxidant taurine on KBrO3-induced damage to the rat intestine. Animals were randomly divided into four groups: control, KBrO3 alone, taurine alone and taurine+ KBrO3. Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered. There was increase in DNA damage and DNA-protein cross-linking. Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect. Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group. These results show that taurine ameliorates bromate induced tissue toxicity and oxidative damage by improving the antioxidant defence, tissue integrity and energy metabolism. Taurine can, therefore, be potentially used as a therapeutic/protective agent against toxicity of KBrO3 and related compounds.

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Related in: MedlinePlus

Histology of hematoxylin and eosin stained sections of rat intestine (duodenum section).Duodenum in the untreated control (A) shows normal epithelia of both villi and intestinal glands whereas KBrO3 treated group (B) reveals extensive damage of both components with the lumen being filled with debris. The taurine alone group (C) shows reasonably well preserved condition of both components while taurine+KBrO3 treated group (D) shows resumption in the morphology of both villi and intestinal glands. Intestinal villi are shown by black arrow and intestinal crypts by green arrow. Magnification is 200 X, scale bar [−] = 50 μm.
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pone.0119137.g003: Histology of hematoxylin and eosin stained sections of rat intestine (duodenum section).Duodenum in the untreated control (A) shows normal epithelia of both villi and intestinal glands whereas KBrO3 treated group (B) reveals extensive damage of both components with the lumen being filled with debris. The taurine alone group (C) shows reasonably well preserved condition of both components while taurine+KBrO3 treated group (D) shows resumption in the morphology of both villi and intestinal glands. Intestinal villi are shown by black arrow and intestinal crypts by green arrow. Magnification is 200 X, scale bar [−] = 50 μm.

Mentions: Histological examination of the duodenum from control animals revealed normal appearance of villi, brush border bearing enterocytes and intestinal crypts containing different types of cells with clear lumen. Marked histological changes were seen in the duodenum of KBrO3-treated rats and extensive intestinal damage was observed. The normal contour of the villi was lost in the form of swelling, oedema and deformation. There was obvious loss and disorganization of surface enterocytes with loss of brush border and infiltration by numerous inflammatory cells. The villus surface is laden with mucus filled goblet cells imparting a vacuolated appearance most obvious in the middle of the villus (Fig. 3). Pre-treatment with taurine greatly attenuated the tissue damage induced by KBrO3. As expected, the taurine alone group showed reasonably well preserved condition of both the components and thus this amino acid does not induce any lesions in the intestine of rats (Fig. 3).


Chemoprotective effect of taurine on potassium bromate-induced DNA damage, DNA-protein cross-linking and oxidative stress in rat intestine.

Ahmad MK, Khan AA, Ali SN, Mahmood R - PLoS ONE (2015)

Histology of hematoxylin and eosin stained sections of rat intestine (duodenum section).Duodenum in the untreated control (A) shows normal epithelia of both villi and intestinal glands whereas KBrO3 treated group (B) reveals extensive damage of both components with the lumen being filled with debris. The taurine alone group (C) shows reasonably well preserved condition of both components while taurine+KBrO3 treated group (D) shows resumption in the morphology of both villi and intestinal glands. Intestinal villi are shown by black arrow and intestinal crypts by green arrow. Magnification is 200 X, scale bar [−] = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352022&req=5

pone.0119137.g003: Histology of hematoxylin and eosin stained sections of rat intestine (duodenum section).Duodenum in the untreated control (A) shows normal epithelia of both villi and intestinal glands whereas KBrO3 treated group (B) reveals extensive damage of both components with the lumen being filled with debris. The taurine alone group (C) shows reasonably well preserved condition of both components while taurine+KBrO3 treated group (D) shows resumption in the morphology of both villi and intestinal glands. Intestinal villi are shown by black arrow and intestinal crypts by green arrow. Magnification is 200 X, scale bar [−] = 50 μm.
Mentions: Histological examination of the duodenum from control animals revealed normal appearance of villi, brush border bearing enterocytes and intestinal crypts containing different types of cells with clear lumen. Marked histological changes were seen in the duodenum of KBrO3-treated rats and extensive intestinal damage was observed. The normal contour of the villi was lost in the form of swelling, oedema and deformation. There was obvious loss and disorganization of surface enterocytes with loss of brush border and infiltration by numerous inflammatory cells. The villus surface is laden with mucus filled goblet cells imparting a vacuolated appearance most obvious in the middle of the villus (Fig. 3). Pre-treatment with taurine greatly attenuated the tissue damage induced by KBrO3. As expected, the taurine alone group showed reasonably well preserved condition of both the components and thus this amino acid does not induce any lesions in the intestine of rats (Fig. 3).

Bottom Line: Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered.Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect.Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT
Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. It induces multiple organ toxicity in humans and experimental animals and is a probable human carcinogen. The present study reports the protective effect of dietary antioxidant taurine on KBrO3-induced damage to the rat intestine. Animals were randomly divided into four groups: control, KBrO3 alone, taurine alone and taurine+ KBrO3. Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered. There was increase in DNA damage and DNA-protein cross-linking. Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect. Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group. These results show that taurine ameliorates bromate induced tissue toxicity and oxidative damage by improving the antioxidant defence, tissue integrity and energy metabolism. Taurine can, therefore, be potentially used as a therapeutic/protective agent against toxicity of KBrO3 and related compounds.

Show MeSH
Related in: MedlinePlus