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Chemoprotective effect of taurine on potassium bromate-induced DNA damage, DNA-protein cross-linking and oxidative stress in rat intestine.

Ahmad MK, Khan AA, Ali SN, Mahmood R - PLoS ONE (2015)

Bottom Line: Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered.Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect.Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT
Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. It induces multiple organ toxicity in humans and experimental animals and is a probable human carcinogen. The present study reports the protective effect of dietary antioxidant taurine on KBrO3-induced damage to the rat intestine. Animals were randomly divided into four groups: control, KBrO3 alone, taurine alone and taurine+ KBrO3. Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered. There was increase in DNA damage and DNA-protein cross-linking. Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect. Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group. These results show that taurine ameliorates bromate induced tissue toxicity and oxidative damage by improving the antioxidant defence, tissue integrity and energy metabolism. Taurine can, therefore, be potentially used as a therapeutic/protective agent against toxicity of KBrO3 and related compounds.

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Related in: MedlinePlus

DNA fragmentation in intestinal mucosal homogenates determined by diphenylamine assay.Results are mean ±SEM of 6 different preparations. * Significantly different at p< 0.05 from control. † Significantly different at p < 0.05 from KBrO3-treated group. ǂ Significantly different at p < 0.05 from KBrO3 treated group
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pone.0119137.g002: DNA fragmentation in intestinal mucosal homogenates determined by diphenylamine assay.Results are mean ±SEM of 6 different preparations. * Significantly different at p< 0.05 from control. † Significantly different at p < 0.05 from KBrO3-treated group. ǂ Significantly different at p < 0.05 from KBrO3 treated group

Mentions: Oral administration of KBrO3, led to significant induction of DPC in intestinal mucosal homogenates (∼3-fold) when compared to the untreated control group (Table 8). It also induced DNA fragmentation (∼2-fold) with release of nucleotides in the supernatant as measured by the diphenylamine assay. Pre-treatment with taurine significantly decreased KBrO3-induced DNA fragmentation and DPC in intestinal mucosa while taurine alone had no effect (Fig. 2).


Chemoprotective effect of taurine on potassium bromate-induced DNA damage, DNA-protein cross-linking and oxidative stress in rat intestine.

Ahmad MK, Khan AA, Ali SN, Mahmood R - PLoS ONE (2015)

DNA fragmentation in intestinal mucosal homogenates determined by diphenylamine assay.Results are mean ±SEM of 6 different preparations. * Significantly different at p< 0.05 from control. † Significantly different at p < 0.05 from KBrO3-treated group. ǂ Significantly different at p < 0.05 from KBrO3 treated group
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352022&req=5

pone.0119137.g002: DNA fragmentation in intestinal mucosal homogenates determined by diphenylamine assay.Results are mean ±SEM of 6 different preparations. * Significantly different at p< 0.05 from control. † Significantly different at p < 0.05 from KBrO3-treated group. ǂ Significantly different at p < 0.05 from KBrO3 treated group
Mentions: Oral administration of KBrO3, led to significant induction of DPC in intestinal mucosal homogenates (∼3-fold) when compared to the untreated control group (Table 8). It also induced DNA fragmentation (∼2-fold) with release of nucleotides in the supernatant as measured by the diphenylamine assay. Pre-treatment with taurine significantly decreased KBrO3-induced DNA fragmentation and DPC in intestinal mucosa while taurine alone had no effect (Fig. 2).

Bottom Line: Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered.Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect.Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, Uttar Pradesh, India.

ABSTRACT
Potassium bromate (KBrO3) is widely used as a food additive and is a major water disinfection by-product. It induces multiple organ toxicity in humans and experimental animals and is a probable human carcinogen. The present study reports the protective effect of dietary antioxidant taurine on KBrO3-induced damage to the rat intestine. Animals were randomly divided into four groups: control, KBrO3 alone, taurine alone and taurine+ KBrO3. Administration of KBrO3 alone led to decrease in the activities of intestinal brush border membrane enzymes while those of antioxidant defence and carbohydrate metabolism were also severely altered. There was increase in DNA damage and DNA-protein cross-linking. Treatment with taurine, prior to administration of KBrO3, resulted in significant attenuation in all these parameters but the administration of taurine alone had no effect. Histological studies supported these biochemical results showing extensive intestinal damage in KBrO3-treated animals and greatly reduced tissue injury in the taurine+ KBrO3 group. These results show that taurine ameliorates bromate induced tissue toxicity and oxidative damage by improving the antioxidant defence, tissue integrity and energy metabolism. Taurine can, therefore, be potentially used as a therapeutic/protective agent against toxicity of KBrO3 and related compounds.

Show MeSH
Related in: MedlinePlus