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Bacterial diversity associated with wild caught Anopheles mosquitoes from Dak Nong Province, Vietnam using culture and DNA fingerprint.

Ngo CT, Aujoulat F, Veas F, Jumas-Bilak E, Manguin S - PLoS ONE (2015)

Bottom Line: The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylum.Four bacterial genera are newly described in Anopheles mosquitoes including Coxiella, Yersinia, Xanthomonas, and Knoellia.The bacterial diversity per specimen was low ranging from 1 to 4.

View Article: PubMed Central - PubMed

Affiliation: Institut de Recherche pour le Développement (IRD), UMR-MD3, Faculté de Pharmacie, Montpellier, France; National Institute of Veterinary Research, Hanoi, Vietnam.

ABSTRACT

Background: Microbiota of Anopheles midgut can modulate vector immunity and block Plasmodium development. Investigation on the bacterial biodiversity in Anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of Africa. Vietnam is an endemic country for both malaria and Bancroftian filariasis whose parasitic agents can be transmitted by the same Anopheles species. No information on the microbiota of Anopheles mosquitoes in Vietnam was available previous to this study.

Method: The culture dependent approach, using different mediums, and culture independent (16S rRNA PCR - TTGE) method were used to investigate the bacterial biodiversity in the abdomen of 5 Anopheles species collected from Dak Nong Province, central-south Vietnam. Molecular methods, sequencing and phylogenetic analysis were used to characterize the microbiota.

Results and discussion: The microbiota in wild-caught Anopheles was diverse with the presence of 47 bacterial OTUs belonging to 30 genera, including bacterial genera impacting Plasmodium development. The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylum. Four bacterial genera are newly described in Anopheles mosquitoes including Coxiella, Yersinia, Xanthomonas, and Knoellia. The bacterial diversity per specimen was low ranging from 1 to 4. The results show the importance of pairing culture and fingerprint methods to better screen the bacterial community in Anopheles mosquitoes.

Conclusion: Sampled Anopheles species from central-south Vietnam contained a diverse bacterial microbiota that needs to be investigated further in order to develop new malaria control approaches. The combination of both culture and DNA fingerprint methods allowed a thorough and complementary screening of the bacterial community in Anopheles mosquitoes.

No MeSH data available.


Related in: MedlinePlus

Prevalence of identified isolates or sequences in bacterial phyla for either TTGE- or culture-based method.Values showed the percentage of positive samples within 52 Anopheles specimens.
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pone.0118634.g001: Prevalence of identified isolates or sequences in bacterial phyla for either TTGE- or culture-based method.Values showed the percentage of positive samples within 52 Anopheles specimens.

Mentions: A total of 28 bacterial strains were isolated and subjected to identification by 16S rRNA gene sequencing. The size of the sequences ranged between 900 and 1,000 bp allowing species-level affiliation in majority of cases. Affiliations into taxonomic levels (species, genus, family, and phylum) are given in Table 2. Microbacterium (Phylum Actinobacteria) present in 6 specimens was the dominant genus of cultivable bacteria in Anopheles sample abdomens, followed by Staphylococcus (Phylum Firmicutes) (5 isolates) and Brachybacterium (Phylum Actinobacteria) (4 isolates) (Table 2). These 3 predominant cultivable genera belong to Gram-positive bacterial phyla, mainly Actinobacteria (Fig. 1). A 16S rRNA gene sequences-based phylogeny was reconstructed. The ML tree in Fig. 2 showed the phylo-taxonomic position of the bacteria isolated in Anopheles. Several isolates were mostly related to uncultured clones and few of them were related to newly described bacterial species [53–57] (Fig. 2).


Bacterial diversity associated with wild caught Anopheles mosquitoes from Dak Nong Province, Vietnam using culture and DNA fingerprint.

Ngo CT, Aujoulat F, Veas F, Jumas-Bilak E, Manguin S - PLoS ONE (2015)

Prevalence of identified isolates or sequences in bacterial phyla for either TTGE- or culture-based method.Values showed the percentage of positive samples within 52 Anopheles specimens.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4352016&req=5

pone.0118634.g001: Prevalence of identified isolates or sequences in bacterial phyla for either TTGE- or culture-based method.Values showed the percentage of positive samples within 52 Anopheles specimens.
Mentions: A total of 28 bacterial strains were isolated and subjected to identification by 16S rRNA gene sequencing. The size of the sequences ranged between 900 and 1,000 bp allowing species-level affiliation in majority of cases. Affiliations into taxonomic levels (species, genus, family, and phylum) are given in Table 2. Microbacterium (Phylum Actinobacteria) present in 6 specimens was the dominant genus of cultivable bacteria in Anopheles sample abdomens, followed by Staphylococcus (Phylum Firmicutes) (5 isolates) and Brachybacterium (Phylum Actinobacteria) (4 isolates) (Table 2). These 3 predominant cultivable genera belong to Gram-positive bacterial phyla, mainly Actinobacteria (Fig. 1). A 16S rRNA gene sequences-based phylogeny was reconstructed. The ML tree in Fig. 2 showed the phylo-taxonomic position of the bacteria isolated in Anopheles. Several isolates were mostly related to uncultured clones and few of them were related to newly described bacterial species [53–57] (Fig. 2).

Bottom Line: The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylum.Four bacterial genera are newly described in Anopheles mosquitoes including Coxiella, Yersinia, Xanthomonas, and Knoellia.The bacterial diversity per specimen was low ranging from 1 to 4.

View Article: PubMed Central - PubMed

Affiliation: Institut de Recherche pour le Développement (IRD), UMR-MD3, Faculté de Pharmacie, Montpellier, France; National Institute of Veterinary Research, Hanoi, Vietnam.

ABSTRACT

Background: Microbiota of Anopheles midgut can modulate vector immunity and block Plasmodium development. Investigation on the bacterial biodiversity in Anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of Africa. Vietnam is an endemic country for both malaria and Bancroftian filariasis whose parasitic agents can be transmitted by the same Anopheles species. No information on the microbiota of Anopheles mosquitoes in Vietnam was available previous to this study.

Method: The culture dependent approach, using different mediums, and culture independent (16S rRNA PCR - TTGE) method were used to investigate the bacterial biodiversity in the abdomen of 5 Anopheles species collected from Dak Nong Province, central-south Vietnam. Molecular methods, sequencing and phylogenetic analysis were used to characterize the microbiota.

Results and discussion: The microbiota in wild-caught Anopheles was diverse with the presence of 47 bacterial OTUs belonging to 30 genera, including bacterial genera impacting Plasmodium development. The bacteria were affiliated with 4 phyla, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, the latter being the dominant phylum. Four bacterial genera are newly described in Anopheles mosquitoes including Coxiella, Yersinia, Xanthomonas, and Knoellia. The bacterial diversity per specimen was low ranging from 1 to 4. The results show the importance of pairing culture and fingerprint methods to better screen the bacterial community in Anopheles mosquitoes.

Conclusion: Sampled Anopheles species from central-south Vietnam contained a diverse bacterial microbiota that needs to be investigated further in order to develop new malaria control approaches. The combination of both culture and DNA fingerprint methods allowed a thorough and complementary screening of the bacterial community in Anopheles mosquitoes.

No MeSH data available.


Related in: MedlinePlus