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Bacterial properties changing under Triton X-100 presence in the diesel oil biodegradation systems: from surface and cellular changes to mono- and dioxygenases activities.

Sałek K, Kaczorek E, Guzik U, Zgoła-Grześkowiak A - Environ Sci Pollut Res Int (2014)

Bottom Line: Triton X-100, as one of the most popular surfactants used in bioremediation techniques, has been reported as an effective agent enhancing the biodegradation of hydrocarbons.However efficient, the surfactant's role in different processes that together enable the satisfying biodegradation should be thoroughly analysed and verified.The addition of various concentrations of Triton X-100 to diesel oil system revealed different cell surface hydrophobicity (CSH) of the tested strains.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965, Poznan, Poland.

ABSTRACT
Triton X-100, as one of the most popular surfactants used in bioremediation techniques, has been reported as an effective agent enhancing the biodegradation of hydrocarbons. However efficient, the surfactant's role in different processes that together enable the satisfying biodegradation should be thoroughly analysed and verified. In this research, we present the interactions of Triton X-100 with the bacterial surfaces (hydrophobicity and zeta potential), its influence on the enzymatic properties (considering mono- and dioxygenases) and profiles of fatty acids, which then all together were compared with the biodegradation rates. The addition of various concentrations of Triton X-100 to diesel oil system revealed different cell surface hydrophobicity (CSH) of the tested strains. The results demonstrated that for Pseudomonas stutzeri strain 9, higher diesel oil biodegradation was correlated with hydrophilic properties of the tested strain and lower Triton X-100 biodegradation. Furthermore, an increase of the branched fatty acids was observed for this strain.

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Diesel oil biodegradation by three bacterial strains, Pseudomonas stutzeri strain 9, Rahnella sp. strain EK12 and Achromobacter sp. 4(2010), and the influence of 120 mg L−1 Triton X-100 on biodegradation. The process was carried out at 25 °C for 7 days. Results have absolute (100 %) quantitative value
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Fig1: Diesel oil biodegradation by three bacterial strains, Pseudomonas stutzeri strain 9, Rahnella sp. strain EK12 and Achromobacter sp. 4(2010), and the influence of 120 mg L−1 Triton X-100 on biodegradation. The process was carried out at 25 °C for 7 days. Results have absolute (100 %) quantitative value

Mentions: In this study, a diesel oil biodegradation by three bacterial strains, Achromobacter sp. 4(2010), P. stutzeri strain 9 and Rahnella sp. strain EK12, after 7 days of experiments was estimated and was followed by the assays of biodegradation in the presence of Triton X-100 (Fig. 1). The laboratory tests with different concentrations of surfactants showed that diesel oil biodegradation was the most effective when 120 mg L−1 of Triton X-100 was used. Diesel oil biodegradation depends not only on the kind of surfactant but also its quality. Therefore, before putting them into the system, the amount of surface active agents should be determined. The use of surfactant in biodegradation processes have to be not only cost effective but also ecologically safe.Fig. 1


Bacterial properties changing under Triton X-100 presence in the diesel oil biodegradation systems: from surface and cellular changes to mono- and dioxygenases activities.

Sałek K, Kaczorek E, Guzik U, Zgoła-Grześkowiak A - Environ Sci Pollut Res Int (2014)

Diesel oil biodegradation by three bacterial strains, Pseudomonas stutzeri strain 9, Rahnella sp. strain EK12 and Achromobacter sp. 4(2010), and the influence of 120 mg L−1 Triton X-100 on biodegradation. The process was carried out at 25 °C for 7 days. Results have absolute (100 %) quantitative value
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4342841&req=5

Fig1: Diesel oil biodegradation by three bacterial strains, Pseudomonas stutzeri strain 9, Rahnella sp. strain EK12 and Achromobacter sp. 4(2010), and the influence of 120 mg L−1 Triton X-100 on biodegradation. The process was carried out at 25 °C for 7 days. Results have absolute (100 %) quantitative value
Mentions: In this study, a diesel oil biodegradation by three bacterial strains, Achromobacter sp. 4(2010), P. stutzeri strain 9 and Rahnella sp. strain EK12, after 7 days of experiments was estimated and was followed by the assays of biodegradation in the presence of Triton X-100 (Fig. 1). The laboratory tests with different concentrations of surfactants showed that diesel oil biodegradation was the most effective when 120 mg L−1 of Triton X-100 was used. Diesel oil biodegradation depends not only on the kind of surfactant but also its quality. Therefore, before putting them into the system, the amount of surface active agents should be determined. The use of surfactant in biodegradation processes have to be not only cost effective but also ecologically safe.Fig. 1

Bottom Line: Triton X-100, as one of the most popular surfactants used in bioremediation techniques, has been reported as an effective agent enhancing the biodegradation of hydrocarbons.However efficient, the surfactant's role in different processes that together enable the satisfying biodegradation should be thoroughly analysed and verified.The addition of various concentrations of Triton X-100 to diesel oil system revealed different cell surface hydrophobicity (CSH) of the tested strains.

View Article: PubMed Central - PubMed

Affiliation: Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo 4, 60-965, Poznan, Poland.

ABSTRACT
Triton X-100, as one of the most popular surfactants used in bioremediation techniques, has been reported as an effective agent enhancing the biodegradation of hydrocarbons. However efficient, the surfactant's role in different processes that together enable the satisfying biodegradation should be thoroughly analysed and verified. In this research, we present the interactions of Triton X-100 with the bacterial surfaces (hydrophobicity and zeta potential), its influence on the enzymatic properties (considering mono- and dioxygenases) and profiles of fatty acids, which then all together were compared with the biodegradation rates. The addition of various concentrations of Triton X-100 to diesel oil system revealed different cell surface hydrophobicity (CSH) of the tested strains. The results demonstrated that for Pseudomonas stutzeri strain 9, higher diesel oil biodegradation was correlated with hydrophilic properties of the tested strain and lower Triton X-100 biodegradation. Furthermore, an increase of the branched fatty acids was observed for this strain.

Show MeSH
Related in: MedlinePlus