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Spatial distribution of bacterial communities driven by multiple environmental factors in a beach wetland of the largest freshwater lake in China.

Ding X, Peng XJ, Jin BS, Xiao M, Chen JK, Li B, Fang CM, Nie M - Front Microbiol (2015)

Bottom Line: Our results showed that bacterial diversity increased along the horizontal gradient and decreased along the vertical gradient.For example, the NH(+) 4 concentration decreased with increasing depth, which was positively correlated with the relative abundance of Alphaproteobacteria.Overall, our results suggest that spatial gradients associated with sediment properties shaped the bacterial communities in the Poyang Lake beach wetland.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Institute of Life Science, Nanchang University Nanchang, China ; School of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing, China.

ABSTRACT
The spatial distributions of bacterial communities may be driven by multiple environmental factors. Thus, understanding the relationships between bacterial distribution and environmental factors is critical for understanding wetland stability and the functioning of freshwater lakes. However, little research on the bacterial communities in deep sediment layers exists. In this study, thirty clone libraries of 16S rRNA were constructed from a beach wetland of the Poyang Lake along both horizontal (distance to the water-land junction) and vertical (sediment depth) gradients to assess the effects of sediment properties on bacterial community structure and diversity. Our results showed that bacterial diversity increased along the horizontal gradient and decreased along the vertical gradient. The heterogeneous sediment properties along gradients substantially affected the dominant bacterial groups at the phylum and species levels. For example, the NH(+) 4 concentration decreased with increasing depth, which was positively correlated with the relative abundance of Alphaproteobacteria. The changes in bacterial diversity and dominant bacterial groups showed that the top layer had a different bacterial community structure than the deeper layers. Principal component analysis revealed that both gradients, not each gradient independently, contributed to the shift in the bacterial community structure. A multiple linear regression model explained the changes in bacterial diversity and richness along the depth and distance gradients. Overall, our results suggest that spatial gradients associated with sediment properties shaped the bacterial communities in the Poyang Lake beach wetland.

No MeSH data available.


Related in: MedlinePlus

Maps of sampling stations. (A) The sampling sites in the Bang Lake of the Poyang Lake wetland. (B) Core sediment samples were collected from a range of beach wetland locations along gradients of depth and distance to the water-land junction. We sampled 5 sediment locations (A, B, C, D and E), which were 0, 125, 250, 375, and 500 m from the water-land junction, respectively. Three 25 × 25 m quadrats (plots) that were 100 m apart were established in each location. From each replicate plot, 10 sediment cores (diameter 2.5 cm) were collected and pooled.
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Figure 1: Maps of sampling stations. (A) The sampling sites in the Bang Lake of the Poyang Lake wetland. (B) Core sediment samples were collected from a range of beach wetland locations along gradients of depth and distance to the water-land junction. We sampled 5 sediment locations (A, B, C, D and E), which were 0, 125, 250, 375, and 500 m from the water-land junction, respectively. Three 25 × 25 m quadrats (plots) that were 100 m apart were established in each location. From each replicate plot, 10 sediment cores (diameter 2.5 cm) were collected and pooled.

Mentions: In this study, we evaluated the spatial distribution of bacterial communities along gradients of both sediment depth and distance to the water-land junction in the Poyang Lake wetland (Figure 1) (Jiangxi Province, mid-China), the largest freshwater lake in China. The aim of this study was to determine whether main bacterial communities are regularly distributed along vertical and horizontal gradients and what environmental factors affect the spatial distributions of bacterial communities. The spatial distribution of the bacterial communities was determined by constructing clone libraries of 16S rRNA and analyzing the associations between the different communities. We hypothesized that different geochemical parameters along vertical and horizontal gradients affect specific bacterial groups in beach sediments.


Spatial distribution of bacterial communities driven by multiple environmental factors in a beach wetland of the largest freshwater lake in China.

Ding X, Peng XJ, Jin BS, Xiao M, Chen JK, Li B, Fang CM, Nie M - Front Microbiol (2015)

Maps of sampling stations. (A) The sampling sites in the Bang Lake of the Poyang Lake wetland. (B) Core sediment samples were collected from a range of beach wetland locations along gradients of depth and distance to the water-land junction. We sampled 5 sediment locations (A, B, C, D and E), which were 0, 125, 250, 375, and 500 m from the water-land junction, respectively. Three 25 × 25 m quadrats (plots) that were 100 m apart were established in each location. From each replicate plot, 10 sediment cores (diameter 2.5 cm) were collected and pooled.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4341555&req=5

Figure 1: Maps of sampling stations. (A) The sampling sites in the Bang Lake of the Poyang Lake wetland. (B) Core sediment samples were collected from a range of beach wetland locations along gradients of depth and distance to the water-land junction. We sampled 5 sediment locations (A, B, C, D and E), which were 0, 125, 250, 375, and 500 m from the water-land junction, respectively. Three 25 × 25 m quadrats (plots) that were 100 m apart were established in each location. From each replicate plot, 10 sediment cores (diameter 2.5 cm) were collected and pooled.
Mentions: In this study, we evaluated the spatial distribution of bacterial communities along gradients of both sediment depth and distance to the water-land junction in the Poyang Lake wetland (Figure 1) (Jiangxi Province, mid-China), the largest freshwater lake in China. The aim of this study was to determine whether main bacterial communities are regularly distributed along vertical and horizontal gradients and what environmental factors affect the spatial distributions of bacterial communities. The spatial distribution of the bacterial communities was determined by constructing clone libraries of 16S rRNA and analyzing the associations between the different communities. We hypothesized that different geochemical parameters along vertical and horizontal gradients affect specific bacterial groups in beach sediments.

Bottom Line: Our results showed that bacterial diversity increased along the horizontal gradient and decreased along the vertical gradient.For example, the NH(+) 4 concentration decreased with increasing depth, which was positively correlated with the relative abundance of Alphaproteobacteria.Overall, our results suggest that spatial gradients associated with sediment properties shaped the bacterial communities in the Poyang Lake beach wetland.

View Article: PubMed Central - PubMed

Affiliation: School of Life Sciences and Institute of Life Science, Nanchang University Nanchang, China ; School of Environmental and Biological Engineering, Nanjing University of Science and Technology Nanjing, China.

ABSTRACT
The spatial distributions of bacterial communities may be driven by multiple environmental factors. Thus, understanding the relationships between bacterial distribution and environmental factors is critical for understanding wetland stability and the functioning of freshwater lakes. However, little research on the bacterial communities in deep sediment layers exists. In this study, thirty clone libraries of 16S rRNA were constructed from a beach wetland of the Poyang Lake along both horizontal (distance to the water-land junction) and vertical (sediment depth) gradients to assess the effects of sediment properties on bacterial community structure and diversity. Our results showed that bacterial diversity increased along the horizontal gradient and decreased along the vertical gradient. The heterogeneous sediment properties along gradients substantially affected the dominant bacterial groups at the phylum and species levels. For example, the NH(+) 4 concentration decreased with increasing depth, which was positively correlated with the relative abundance of Alphaproteobacteria. The changes in bacterial diversity and dominant bacterial groups showed that the top layer had a different bacterial community structure than the deeper layers. Principal component analysis revealed that both gradients, not each gradient independently, contributed to the shift in the bacterial community structure. A multiple linear regression model explained the changes in bacterial diversity and richness along the depth and distance gradients. Overall, our results suggest that spatial gradients associated with sediment properties shaped the bacterial communities in the Poyang Lake beach wetland.

No MeSH data available.


Related in: MedlinePlus