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Effects of methionine supplementation on the expression of protein deposition-related genes in acute heat stress-exposed broilers.

Del Vesco AP, Gasparino E, Grieser DO, Zancanela V, Voltolini DM, Khatlab AS, Guimarães SE, Soares MA, Oliveira Neto AR - PLoS ONE (2015)

Bottom Line: Grower period: Significant effects for the interaction between supplementation and environment were observed for GHR (P = 0.0252) and CTSL2 (P = 0.0011) gene expression.Only methionine supplementation had effect on atrogin-1 gene expression (P<0.0001), with higher methionine content in the diet lower atrogin-1 gene expression was observed.Our results suggest that heat stress induces greater protein degradation and that methionine supplementation could induce protein deposition because methionine increased the expression of genes related to protein synthesis and decreased the expression of genes related to protein breakdown.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.

ABSTRACT
The objective of this study was to evaluate the effect of heat stress and methionine supplementation on the gene expression of insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), phosphatidylinositol 3-kinase, and regulatory 1 (PI3KR1) in the liver, as well as the expression of the atrogin 1 and cathepsin L2 (CTSL2) genes in the breast muscle of broilers. Broilers from 1-21 and 22-42 days of age were divided into three treatments related to methionine supplementation as follows: without methionine supplementation (MD), recommended level of methionine (DL1), and excess supplementation of methionine (DL2). The animals were either maintained at a thermal comfort temperature or exposed to heat stress (HS) (38°C for 24 hours, starting on day 20 or day 41 for experiments 1 and 2, respectively). The heat stress increased the body temperature at both ages. Starter period: The HS animals presented increased plasma creatinine content (P<0.0001) and the highest CTSL2 gene expression (P<0.0001). The methionine supplementation increased the IGF-I (P = 0.0144) and GHR (P = 0.0011) gene expression and decreased the CTSL2 (P = 0.0004) and atrogin 1 (P = 0.0012) gene expression. Grower period: Significant effects for the interaction between supplementation and environment were observed for GHR (P = 0.0252) and CTSL2 (P = 0.0011) gene expression. The highest GHR expression was observed in animals that remained in thermal comfort on the DL2 diet, and the lowest expression occurred in the HS animals fed the MD diet. For CTSL2, the HS animals fed the MD diet presented the highest CTSL2 gene expression, and the lowest expression was observed in the animals maintained at thermal comfort on DL1 and DL2 diets. Only methionine supplementation had effect on atrogin-1 gene expression (P<0.0001), with higher methionine content in the diet lower atrogin-1 gene expression was observed. Our results suggest that heat stress induces greater protein degradation and that methionine supplementation could induce protein deposition because methionine increased the expression of genes related to protein synthesis and decreased the expression of genes related to protein breakdown.

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Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.
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pone.0115821.g001: Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.

Mentions: No effect of the interaction between methionine supplementation and temperature was observed on body temperature or creatinine content (Fig. 1). We observed that acute heat stress (38°C for 24 hours) was sufficient to increase the birds' body temperature in both the experiments, with 40.31°C ± 0.07 (thermal comfort) vs. 41.87°C ± 0.17 (HS) (P<0.0001) for animals in the starter period and 41.35°C ± 0.19 (thermal comfort) vs. 42.78°C ± 0.12 (HS) (P<0.0001) for animals in the grower phase. HS animals from the starter phase also presented higher creatinine content (P<0.0001).


Effects of methionine supplementation on the expression of protein deposition-related genes in acute heat stress-exposed broilers.

Del Vesco AP, Gasparino E, Grieser DO, Zancanela V, Voltolini DM, Khatlab AS, Guimarães SE, Soares MA, Oliveira Neto AR - PLoS ONE (2015)

Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340924&req=5

pone.0115821.g001: Effects of environment and methionine supplementation on creatinine content and body temperature of broilers from the starter (A and C) and grower (B and D) periods.
Mentions: No effect of the interaction between methionine supplementation and temperature was observed on body temperature or creatinine content (Fig. 1). We observed that acute heat stress (38°C for 24 hours) was sufficient to increase the birds' body temperature in both the experiments, with 40.31°C ± 0.07 (thermal comfort) vs. 41.87°C ± 0.17 (HS) (P<0.0001) for animals in the starter period and 41.35°C ± 0.19 (thermal comfort) vs. 42.78°C ± 0.12 (HS) (P<0.0001) for animals in the grower phase. HS animals from the starter phase also presented higher creatinine content (P<0.0001).

Bottom Line: Grower period: Significant effects for the interaction between supplementation and environment were observed for GHR (P = 0.0252) and CTSL2 (P = 0.0011) gene expression.Only methionine supplementation had effect on atrogin-1 gene expression (P<0.0001), with higher methionine content in the diet lower atrogin-1 gene expression was observed.Our results suggest that heat stress induces greater protein degradation and that methionine supplementation could induce protein deposition because methionine increased the expression of genes related to protein synthesis and decreased the expression of genes related to protein breakdown.

View Article: PubMed Central - PubMed

Affiliation: Department of Animal Science, Universidade Estadual de Maringá-UEM-Maringá, Paraná, Brazil.

ABSTRACT
The objective of this study was to evaluate the effect of heat stress and methionine supplementation on the gene expression of insulin-like growth factor I (IGF-I), growth hormone receptor (GHR), phosphatidylinositol 3-kinase, and regulatory 1 (PI3KR1) in the liver, as well as the expression of the atrogin 1 and cathepsin L2 (CTSL2) genes in the breast muscle of broilers. Broilers from 1-21 and 22-42 days of age were divided into three treatments related to methionine supplementation as follows: without methionine supplementation (MD), recommended level of methionine (DL1), and excess supplementation of methionine (DL2). The animals were either maintained at a thermal comfort temperature or exposed to heat stress (HS) (38°C for 24 hours, starting on day 20 or day 41 for experiments 1 and 2, respectively). The heat stress increased the body temperature at both ages. Starter period: The HS animals presented increased plasma creatinine content (P<0.0001) and the highest CTSL2 gene expression (P<0.0001). The methionine supplementation increased the IGF-I (P = 0.0144) and GHR (P = 0.0011) gene expression and decreased the CTSL2 (P = 0.0004) and atrogin 1 (P = 0.0012) gene expression. Grower period: Significant effects for the interaction between supplementation and environment were observed for GHR (P = 0.0252) and CTSL2 (P = 0.0011) gene expression. The highest GHR expression was observed in animals that remained in thermal comfort on the DL2 diet, and the lowest expression occurred in the HS animals fed the MD diet. For CTSL2, the HS animals fed the MD diet presented the highest CTSL2 gene expression, and the lowest expression was observed in the animals maintained at thermal comfort on DL1 and DL2 diets. Only methionine supplementation had effect on atrogin-1 gene expression (P<0.0001), with higher methionine content in the diet lower atrogin-1 gene expression was observed. Our results suggest that heat stress induces greater protein degradation and that methionine supplementation could induce protein deposition because methionine increased the expression of genes related to protein synthesis and decreased the expression of genes related to protein breakdown.

Show MeSH
Related in: MedlinePlus