Limits...
Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

Show MeSH
Mean (±SD) plasma concentration–time profiles of 3MBM and 4MBM administered to 12 Sprague–Dawley rats.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4340794&req=5

pone.0116010.g004: Mean (±SD) plasma concentration–time profiles of 3MBM and 4MBM administered to 12 Sprague–Dawley rats.

Mentions: The validated analytical method was first employed to study the PK parameters of 3MBM and 4MBM in rats. The mean plasma concentration-time curves following oral administration of 3MBM or 4MBM are presented in Fig. 4.


Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Mean (±SD) plasma concentration–time profiles of 3MBM and 4MBM administered to 12 Sprague–Dawley rats.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340794&req=5

pone.0116010.g004: Mean (±SD) plasma concentration–time profiles of 3MBM and 4MBM administered to 12 Sprague–Dawley rats.
Mentions: The validated analytical method was first employed to study the PK parameters of 3MBM and 4MBM in rats. The mean plasma concentration-time curves following oral administration of 3MBM or 4MBM are presented in Fig. 4.

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

Show MeSH