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Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

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Representative SRM chromatograms of PPD (the IS, I), 3MBM (II) and 4MBM (III) in rat plasma samples: (a) a blank rat plasma sample; (b) a blank rat plasma sample spiked with PPD (1000 ng/ml), 3MBM (5 ng/ml), or 4MBM (5 ng/ml); (C) a rat plasma sample collected 90 min after an oral dose of 3MBM (II) and 4MBM (III) administered to a Sprague–Dawley rat.
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pone.0116010.g003: Representative SRM chromatograms of PPD (the IS, I), 3MBM (II) and 4MBM (III) in rat plasma samples: (a) a blank rat plasma sample; (b) a blank rat plasma sample spiked with PPD (1000 ng/ml), 3MBM (5 ng/ml), or 4MBM (5 ng/ml); (C) a rat plasma sample collected 90 min after an oral dose of 3MBM (II) and 4MBM (III) administered to a Sprague–Dawley rat.

Mentions: Specificity. The interference of endogenous plasma constituents with 3MBM, 4MBM and the IS was assessed by inspecting the chromatograms derived from the processed blank plasma samples. Typical chromatograms obtained from blank plasma, blank plasma spiked with a target analyte and the IS, and plasma samples after administration of a target analyte are presented in Fig. 3. The retention times of PPD (IS), 3MBM and 4MBM were approximately 0.92, 1.36 and 8.57 min, respectively. There was no endogenous interference or matrix effect on ionization.


Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Representative SRM chromatograms of PPD (the IS, I), 3MBM (II) and 4MBM (III) in rat plasma samples: (a) a blank rat plasma sample; (b) a blank rat plasma sample spiked with PPD (1000 ng/ml), 3MBM (5 ng/ml), or 4MBM (5 ng/ml); (C) a rat plasma sample collected 90 min after an oral dose of 3MBM (II) and 4MBM (III) administered to a Sprague–Dawley rat.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340794&req=5

pone.0116010.g003: Representative SRM chromatograms of PPD (the IS, I), 3MBM (II) and 4MBM (III) in rat plasma samples: (a) a blank rat plasma sample; (b) a blank rat plasma sample spiked with PPD (1000 ng/ml), 3MBM (5 ng/ml), or 4MBM (5 ng/ml); (C) a rat plasma sample collected 90 min after an oral dose of 3MBM (II) and 4MBM (III) administered to a Sprague–Dawley rat.
Mentions: Specificity. The interference of endogenous plasma constituents with 3MBM, 4MBM and the IS was assessed by inspecting the chromatograms derived from the processed blank plasma samples. Typical chromatograms obtained from blank plasma, blank plasma spiked with a target analyte and the IS, and plasma samples after administration of a target analyte are presented in Fig. 3. The retention times of PPD (IS), 3MBM and 4MBM were approximately 0.92, 1.36 and 8.57 min, respectively. There was no endogenous interference or matrix effect on ionization.

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

Show MeSH