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Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

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Precursor ion and product ion spectra of 3MBM (a), 4MBM (b) and PPD (c).
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pone.0116010.g002: Precursor ion and product ion spectra of 3MBM (a), 4MBM (b) and PPD (c).

Mentions: The ESI source provided a better response than the APCI source for both analytes. In the precursor ion full-scan spectra, the most abundant ions were protonated molecules [M+H]+ at m/z 353, 353 and 166 for 3MBM, 4MBM and the IS, respectively. The MS parameters, such as desolvation temperature, ESI source temperature, capillary and spray voltage, and flow rate of the desolvation gas and auxiliary gas, were optimized to obtain the highest intensities of the protonated molecules of the analytes and the IS. The product ion scan spectra displayed high-abundance fragment ions at m/z 148, 148 and 133 for 3MBM, 4MBM and the IS, respectively (Fig. 2). The collision gas pressure and collision energy of collision-induced decomposition (CID) were optimized to result in maximum responses for the fragmentation of the two analytes. SRM was performed on the precursor → product ion transition at m/z 343 → 148 for 3MBM, m/z 343 → 148 for 4MBM and m/z 166 → 133 for the IS (Fig. 2).


Pharmacokinetic study of 14-(3-methylbenzyl)matrine and 14-(4-methylbenzyl)matrine in rat plasma using liquid chromatography-tandem mass spectrometry.

Jiang M, Wang L, Huang S, Xu L, Hu C, Jiang W - PLoS ONE (2015)

Precursor ion and product ion spectra of 3MBM (a), 4MBM (b) and PPD (c).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340794&req=5

pone.0116010.g002: Precursor ion and product ion spectra of 3MBM (a), 4MBM (b) and PPD (c).
Mentions: The ESI source provided a better response than the APCI source for both analytes. In the precursor ion full-scan spectra, the most abundant ions were protonated molecules [M+H]+ at m/z 353, 353 and 166 for 3MBM, 4MBM and the IS, respectively. The MS parameters, such as desolvation temperature, ESI source temperature, capillary and spray voltage, and flow rate of the desolvation gas and auxiliary gas, were optimized to obtain the highest intensities of the protonated molecules of the analytes and the IS. The product ion scan spectra displayed high-abundance fragment ions at m/z 148, 148 and 133 for 3MBM, 4MBM and the IS, respectively (Fig. 2). The collision gas pressure and collision energy of collision-induced decomposition (CID) were optimized to result in maximum responses for the fragmentation of the two analytes. SRM was performed on the precursor → product ion transition at m/z 343 → 148 for 3MBM, m/z 343 → 148 for 4MBM and m/z 166 → 133 for the IS (Fig. 2).

Bottom Line: Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard.The proposed method enables the unambiguous identification and quantification of these two compounds in vivo.These results provide a meaningful basis for evaluating the clinical applications of these medicines.

View Article: PubMed Central - PubMed

Affiliation: School of Chemistry and Chemical Engineering, Guangxi University, 100 Daxue Road, Nanning, 530004, China.

ABSTRACT
A rapid, sensitive and selective high-performance liquid chromatography-tandem mass spectrometric method (HPLC-MS) was developed and validated to determine the 14-(3-methylbenzyl)matrine (3MBM) and 14-(4-methylbenzyl)matrine (4MBM) levels in rat plasma in the present study. The analytes were separated using a C18 column (1.9 μm, 2.1 mm × 100 mm) equipped with a Security Guard C18 column (5 μm, 2.1 mm × 10 mm), followed by detection via triple-quadrupole mass spectrometry using an electrospray ionization (ESI) source. Sample pretreatment involved one-step protein precipitation with isopropanol:ethyl acetate (v/v, 25:75), and pseudoephedrine hydrochloride was used as an internal standard. The method was linear in the concentration range of 5-2000 ng/ml for both compounds. The intra-day and inter-day relative standard deviations (RSDs) were less than 15%, and all relative errors (REs) were within 15%. The proposed method enables the unambiguous identification and quantification of these two compounds in vivo. This study is the first to determine the 3MBM and 4MBM levels in rat plasma after oral administration of these compounds. These results provide a meaningful basis for evaluating the clinical applications of these medicines.

Show MeSH