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Pseudomonas aeruginosa resistance phenotypes and phenotypic highlighting methods.

Bălăşoiu M, Bălăşoiu AT, Mănescu R, Avramescu C, Ionete O - Curr Health Sci J (2014)

Bottom Line: The most important resistance mechanisms are: enzyme production, reduction of pore expression, reduction of the external membrane proteins expression, efflux systems, topoisomerase mutations.The most frequent acquired resistance mechanisms are betalactamase-type enzyme production (ESBLs, AmpC, carbapenemases), which determine variable phenotypes of betalactamines resistance, phenotypes which are associated with aminoglycosides and quinolones resistance.The nonenzymatic drug resistance mechanisms are caused by efflux systems, pore reduction and penicillin-binding proteins (PBP) modification, which are often associated to other resistance mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology, Virusology, Parasitology, UMF of Craiova.

ABSTRACT
Pseudomonas aeruginosa genus bacteria are well known for their increased drug resistance (phenotypic ang genotypic resistance). The most important resistance mechanisms are: enzyme production, reduction of pore expression, reduction of the external membrane proteins expression, efflux systems, topoisomerase mutations. These mechanisms often accumulate and lead to multidrug ressitance strains emergence. The most frequent acquired resistance mechanisms are betalactamase-type enzyme production (ESBLs, AmpC, carbapenemases), which determine variable phenotypes of betalactamines resistance, phenotypes which are associated with aminoglycosides and quinolones resistance. The nonenzymatic drug resistance mechanisms are caused by efflux systems, pore reduction and penicillin-binding proteins (PBP) modification, which are often associated to other resistance mechanisms. Phenotypic methods used for testing these mechanisms are based on highlighting these phenotypes using Kirby Bauer antibiogram, clinical breakpoints, and "cut off" values recommended by EUCAST 2013 standard, version 3.1.

No MeSH data available.


Related in: MedlinePlus

Double disc method
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Figure 1: Double disc method

Mentions: a.Double disk method (synergy test): a disc containing amoxicillin+clavulanic acid (20 μg+10 μg) is placed in the center of a Mueller-Hinton gelose medium plate and at about 20-35 mm from this central disc, other discs containing ceftazidime, ceftriaxone, cefotaxime are placed (champagne stopper method) (Fig. 1).


Pseudomonas aeruginosa resistance phenotypes and phenotypic highlighting methods.

Bălăşoiu M, Bălăşoiu AT, Mănescu R, Avramescu C, Ionete O - Curr Health Sci J (2014)

Double disc method
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340447&req=5

Figure 1: Double disc method
Mentions: a.Double disk method (synergy test): a disc containing amoxicillin+clavulanic acid (20 μg+10 μg) is placed in the center of a Mueller-Hinton gelose medium plate and at about 20-35 mm from this central disc, other discs containing ceftazidime, ceftriaxone, cefotaxime are placed (champagne stopper method) (Fig. 1).

Bottom Line: The most important resistance mechanisms are: enzyme production, reduction of pore expression, reduction of the external membrane proteins expression, efflux systems, topoisomerase mutations.The most frequent acquired resistance mechanisms are betalactamase-type enzyme production (ESBLs, AmpC, carbapenemases), which determine variable phenotypes of betalactamines resistance, phenotypes which are associated with aminoglycosides and quinolones resistance.The nonenzymatic drug resistance mechanisms are caused by efflux systems, pore reduction and penicillin-binding proteins (PBP) modification, which are often associated to other resistance mechanisms.

View Article: PubMed Central - PubMed

Affiliation: Department of Bacteriology, Virusology, Parasitology, UMF of Craiova.

ABSTRACT
Pseudomonas aeruginosa genus bacteria are well known for their increased drug resistance (phenotypic ang genotypic resistance). The most important resistance mechanisms are: enzyme production, reduction of pore expression, reduction of the external membrane proteins expression, efflux systems, topoisomerase mutations. These mechanisms often accumulate and lead to multidrug ressitance strains emergence. The most frequent acquired resistance mechanisms are betalactamase-type enzyme production (ESBLs, AmpC, carbapenemases), which determine variable phenotypes of betalactamines resistance, phenotypes which are associated with aminoglycosides and quinolones resistance. The nonenzymatic drug resistance mechanisms are caused by efflux systems, pore reduction and penicillin-binding proteins (PBP) modification, which are often associated to other resistance mechanisms. Phenotypic methods used for testing these mechanisms are based on highlighting these phenotypes using Kirby Bauer antibiogram, clinical breakpoints, and "cut off" values recommended by EUCAST 2013 standard, version 3.1.

No MeSH data available.


Related in: MedlinePlus