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First imported Plasmodium ovale malaria in Central America: case report of a Guatemalan soldier and a call to improve its accurate diagnosis.

Castellanos ME, Díaz S, Parsons E, Peruski LF, Enríquez F, Ramírez JL, Padilla N - Mil Med Res (2015)

Bottom Line: The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region.Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence.We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa.

View Article: PubMed Central - PubMed

Affiliation: Center for Health Studies-Universidad del Valle de Guatemala (CES-UVG), 18 Av. 11-95 zona 15, Vista Hermosa III, Guatemala City, CA Guatemala.

ABSTRACT
The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region. Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa. The obstacles for its diagnosis using the standard microscopy technique and the need to improve its detection are discussed.

No MeSH data available.


Related in: MedlinePlus

Plasmodium ovaleparasites (initially diagnosed asP. vivax) shown in different stages in the blood smear of the patient at time of presentation (Giemsa Stain, X 1000). A and C: ring-form trophozoites. B and D: gametocytes.
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Fig2: Plasmodium ovaleparasites (initially diagnosed asP. vivax) shown in different stages in the blood smear of the patient at time of presentation (Giemsa Stain, X 1000). A and C: ring-form trophozoites. B and D: gametocytes.

Mentions: Based on the travel history of the patient and the doubts arisen as to its species identity, molecular testing was conducted to confirm the Plasmodium species. A P. ovale mono-infection was identified on both the blood film and whole blood of the patient using a Plasmodium genus-specific nested PCR (Figure 1) and a SYBR Green-based quantitative real-time PCR [16,17]. The blood film was re-examined to identify the morphological features of this parasite (Figure 2). Sequence analysis was conducted to discriminate between the classic or variant P. ovale. Basic local alignment search tool (BLAST) analysis revealed 95% similarity with P. ovale ssrRNA gene. The strain was classified as the classic type, P. ovale curtisi, based on its nucleotide polymorphisms [5]. The Genetics and Immunology Laboratory, Malaria Branch at Centers for Disease Control (Atlanta, US) confirmed these findings.Figure 1


First imported Plasmodium ovale malaria in Central America: case report of a Guatemalan soldier and a call to improve its accurate diagnosis.

Castellanos ME, Díaz S, Parsons E, Peruski LF, Enríquez F, Ramírez JL, Padilla N - Mil Med Res (2015)

Plasmodium ovaleparasites (initially diagnosed asP. vivax) shown in different stages in the blood smear of the patient at time of presentation (Giemsa Stain, X 1000). A and C: ring-form trophozoites. B and D: gametocytes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4340331&req=5

Fig2: Plasmodium ovaleparasites (initially diagnosed asP. vivax) shown in different stages in the blood smear of the patient at time of presentation (Giemsa Stain, X 1000). A and C: ring-form trophozoites. B and D: gametocytes.
Mentions: Based on the travel history of the patient and the doubts arisen as to its species identity, molecular testing was conducted to confirm the Plasmodium species. A P. ovale mono-infection was identified on both the blood film and whole blood of the patient using a Plasmodium genus-specific nested PCR (Figure 1) and a SYBR Green-based quantitative real-time PCR [16,17]. The blood film was re-examined to identify the morphological features of this parasite (Figure 2). Sequence analysis was conducted to discriminate between the classic or variant P. ovale. Basic local alignment search tool (BLAST) analysis revealed 95% similarity with P. ovale ssrRNA gene. The strain was classified as the classic type, P. ovale curtisi, based on its nucleotide polymorphisms [5]. The Genetics and Immunology Laboratory, Malaria Branch at Centers for Disease Control (Atlanta, US) confirmed these findings.Figure 1

Bottom Line: The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region.Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence.We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa.

View Article: PubMed Central - PubMed

Affiliation: Center for Health Studies-Universidad del Valle de Guatemala (CES-UVG), 18 Av. 11-95 zona 15, Vista Hermosa III, Guatemala City, CA Guatemala.

ABSTRACT
The Mesoamerican Ministers of Health have set 2020 as the target for malaria elimination to be achieved in the region. Imported malaria cases are a potential threat to countries attempting elimination or working to prevent resurgence. We report the first imported Plasmodium ovale infection with molecular confirmation in Central America, which occurred in a Guatemalan soldier that had been deployed in Africa. The obstacles for its diagnosis using the standard microscopy technique and the need to improve its detection are discussed.

No MeSH data available.


Related in: MedlinePlus