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The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell.

Sun Y, Ning T, Liu Z, Pang J, Jiang D, Guo Z, Song G, Yang D - BMC Plant Biol. (2015)

Bottom Line: In the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast.Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies.Our results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sec18p/N-ethylmaleimide-sensitive factor (NSF) is a conserved eukaryotic ATPase, which primarily functions in vesicle membrane fusion from yeast to human. However, the function of the OsSec18 gene, a homologue of NSF in rice, remains unknown.

Results: In the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast. Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies. Further examination revealed that OsSec18 presented as a 290-kDa complex in rice endosperm cells. Moreover, Os60sP0 was identified a component of this complex, demonstrating that the OsSec18 complex contains another complex of P0(P1-P2)2 in rice endosperm cells. Furthermore, we determined that the N-terminus of OsSec18 can interact with the N- and C-termini of Os60sP0, whereas the C-terminus of OsSec18 can only interact with the C-terminus of Os60sP0.

Conclusion: Our results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell.

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Tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.
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Fig1: Tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.

Mentions: To explore the expression profile of OsSec18 in rice, we analyzed various tissues and organs via Western blot analysis. The results revealed that OsSec18 expressed in leaf, stem, inflorescence, and immature and mature seeds but not in root. The highest expression level was found in stem, inflorescence and immature seed (FigureĀ 1).Figure 1


The OsSec18 complex interacts with P0(P1-P2)2 to regulate vacuolar morphology in rice endosperm cell.

Sun Y, Ning T, Liu Z, Pang J, Jiang D, Guo Z, Song G, Yang D - BMC Plant Biol. (2015)

Tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4340293&req=5

Fig1: Tissue-specific expression patterns of the OsSec18 protein. R, root; ST, stem; L, leaf; IF, inflorescence; IMS, immature seed; MS, mature seed.
Mentions: To explore the expression profile of OsSec18 in rice, we analyzed various tissues and organs via Western blot analysis. The results revealed that OsSec18 expressed in leaf, stem, inflorescence, and immature and mature seeds but not in root. The highest expression level was found in stem, inflorescence and immature seed (FigureĀ 1).Figure 1

Bottom Line: In the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast.Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies.Our results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell.

View Article: PubMed Central - PubMed

ABSTRACT

Background: Sec18p/N-ethylmaleimide-sensitive factor (NSF) is a conserved eukaryotic ATPase, which primarily functions in vesicle membrane fusion from yeast to human. However, the function of the OsSec18 gene, a homologue of NSF in rice, remains unknown.

Results: In the present study, we investigated the function of OsSec18 in rice and found that OsSec18 complements the temperature-sensitive phenotype and interferes with vacuolar morphogenesis in yeast. Overexpression of OsSec18 in rice decreased the plant height and 1000-grain weight and altered the morphology of the protein bodies. Further examination revealed that OsSec18 presented as a 290-kDa complex in rice endosperm cells. Moreover, Os60sP0 was identified a component of this complex, demonstrating that the OsSec18 complex contains another complex of P0(P1-P2)2 in rice endosperm cells. Furthermore, we determined that the N-terminus of OsSec18 can interact with the N- and C-termini of Os60sP0, whereas the C-terminus of OsSec18 can only interact with the C-terminus of Os60sP0.

Conclusion: Our results revealed that the OsSec18 regulates vacuolar morphology in both yeast and rice endosperm cell and the OsSec18 interacts with P0(P1-P2)2 complex in rice endosperm cell.

Show MeSH
Related in: MedlinePlus