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ROSics: chemistry and proteomics of cysteine modifications in redox biology.

Kim HJ, Ha S, Lee HY, Lee KJ - Mass Spectrom Rev (2014)

Bottom Line: It is therefore necessary to identify the Cys-containing ROS target proteins, as well as the sites and species of their PTMs. Cutting edge proteomic tools which have helped identify the PTMs at reactive Cys residues, have also revealed that Cys residues are modified in numerous ways.We present here, a review of relevant ROS biology, possible chemical reactions of Cys residues and details of the proteomic strategies employed for rapid, efficient and sensitive identification of diverse and novel PTMs involving reactive Cys residues of redox-sensitive proteins.We propose a new name, "ROSics," for the science which describes the principles of mode of action of ROS at molecular levels.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, 120-750, Korea.

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Four alternate modes of elimination of Cys to dehydroalanine (DHA) (a) reduction-elimination of Cys-disulfides to DHA, (b) base-mediated elimination of Cys-disulfides to DHA, (c) oxidative elimination of Cys to DHA, (d) bis-alkylation elimination of Cys to DHA.
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sch14: Four alternate modes of elimination of Cys to dehydroalanine (DHA) (a) reduction-elimination of Cys-disulfides to DHA, (b) base-mediated elimination of Cys-disulfides to DHA, (c) oxidative elimination of Cys to DHA, (d) bis-alkylation elimination of Cys to DHA.


ROSics: chemistry and proteomics of cysteine modifications in redox biology.

Kim HJ, Ha S, Lee HY, Lee KJ - Mass Spectrom Rev (2014)

Four alternate modes of elimination of Cys to dehydroalanine (DHA) (a) reduction-elimination of Cys-disulfides to DHA, (b) base-mediated elimination of Cys-disulfides to DHA, (c) oxidative elimination of Cys to DHA, (d) bis-alkylation elimination of Cys to DHA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340047&req=5

sch14: Four alternate modes of elimination of Cys to dehydroalanine (DHA) (a) reduction-elimination of Cys-disulfides to DHA, (b) base-mediated elimination of Cys-disulfides to DHA, (c) oxidative elimination of Cys to DHA, (d) bis-alkylation elimination of Cys to DHA.
Bottom Line: It is therefore necessary to identify the Cys-containing ROS target proteins, as well as the sites and species of their PTMs. Cutting edge proteomic tools which have helped identify the PTMs at reactive Cys residues, have also revealed that Cys residues are modified in numerous ways.We present here, a review of relevant ROS biology, possible chemical reactions of Cys residues and details of the proteomic strategies employed for rapid, efficient and sensitive identification of diverse and novel PTMs involving reactive Cys residues of redox-sensitive proteins.We propose a new name, "ROSics," for the science which describes the principles of mode of action of ROS at molecular levels.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Pharmaceutical Sciences, College of Pharmacy, Ewha Womans University, Seoul, 120-750, Korea.

Show MeSH
Related in: MedlinePlus