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Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients.

Roberts HM, Ling MR, Insall R, Kalna G, Spengler J, Grant MM, Chapple IL - J. Clin. Periodontol. (2015)

Bottom Line: Circular statistics were utilized for the analysis of cell movement.However, velocity and accuracy were normalized for the weak chemoattractant CXCL8 while they remained significantly reduced for fMLP.Dysfunctional neutrophil chemotaxis may predispose patients with periodontitis to their disease by increasing tissue transit times, thus exacerbating neutrophil-mediated collateral host tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Periodontal Research Group and MRC Centre for Immune Regulation, University of Birmingham, Birmingham, UK.

No MeSH data available.


Related in: MedlinePlus

Summary of post-treatment results. The diagrams illustrate three plots per condition, as described in Fig. 3.
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fig04: Summary of post-treatment results. The diagrams illustrate three plots per condition, as described in Fig. 3.

Mentions: XY coordinates of the cells were generated using the Manual tracking Plugin and ImageJ software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA) and these were further analysed with the circular statistics (CircStat) toolbox from MATLAB (Mathworks, Natick, MA, USA) software to ascertain the significance of the cells’ movement over the time course. The CircStat toolbox provides statistics for directional data, including the mean direction, known as the resultant vector, the length of which indicates the strength of the direction taken by the cells. Results are represented as circular diagrams. Two representations are shown (Figs 3 and 4): (1) resultant vector plots showing the distribution of the final angle of all cells in the experiment with a vector line showing the mean angle and vector length, illustrating the strength of the movement; and (2) rose plots showing the proportion of cells in each of 18 segments around the circle, the larger the bar the greater proportion of cells that moved in that direction. Data were further summarized in box and whisker plots, and statistical analysis of these was performed by Wilcoxon test using Prism 5.0 software (GraphPad, San Diego, California USA). Volunteer age was compared by paired t-test and probing pocket depths by repeated measures anova followed by Tukey–Kramer multiple comparisons test. The number of probing pocket depths >4 mm, percentage bleeding on probing, and gingival and plaque indices were compared using Friedman test followed by Dunn's multiple comparisons test.


Impaired neutrophil directional chemotactic accuracy in chronic periodontitis patients.

Roberts HM, Ling MR, Insall R, Kalna G, Spengler J, Grant MM, Chapple IL - J. Clin. Periodontol. (2015)

Summary of post-treatment results. The diagrams illustrate three plots per condition, as described in Fig. 3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340045&req=5

fig04: Summary of post-treatment results. The diagrams illustrate three plots per condition, as described in Fig. 3.
Mentions: XY coordinates of the cells were generated using the Manual tracking Plugin and ImageJ software (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA) and these were further analysed with the circular statistics (CircStat) toolbox from MATLAB (Mathworks, Natick, MA, USA) software to ascertain the significance of the cells’ movement over the time course. The CircStat toolbox provides statistics for directional data, including the mean direction, known as the resultant vector, the length of which indicates the strength of the direction taken by the cells. Results are represented as circular diagrams. Two representations are shown (Figs 3 and 4): (1) resultant vector plots showing the distribution of the final angle of all cells in the experiment with a vector line showing the mean angle and vector length, illustrating the strength of the movement; and (2) rose plots showing the proportion of cells in each of 18 segments around the circle, the larger the bar the greater proportion of cells that moved in that direction. Data were further summarized in box and whisker plots, and statistical analysis of these was performed by Wilcoxon test using Prism 5.0 software (GraphPad, San Diego, California USA). Volunteer age was compared by paired t-test and probing pocket depths by repeated measures anova followed by Tukey–Kramer multiple comparisons test. The number of probing pocket depths >4 mm, percentage bleeding on probing, and gingival and plaque indices were compared using Friedman test followed by Dunn's multiple comparisons test.

Bottom Line: Circular statistics were utilized for the analysis of cell movement.However, velocity and accuracy were normalized for the weak chemoattractant CXCL8 while they remained significantly reduced for fMLP.Dysfunctional neutrophil chemotaxis may predispose patients with periodontitis to their disease by increasing tissue transit times, thus exacerbating neutrophil-mediated collateral host tissue damage.

View Article: PubMed Central - PubMed

Affiliation: Periodontal Research Group and MRC Centre for Immune Regulation, University of Birmingham, Birmingham, UK.

No MeSH data available.


Related in: MedlinePlus