Limits...
Effects of cytokines and chemokines on migration of mesenchymal stem cells following spinal cord injury.

Li L, Yang M, Wang C, Zhao Q, Liu J, Zhan C, Liu Z, Li X, Wang W, Yang X - Neural Regen Res (2012)

Bottom Line: We investigated the effects of cytokines and chemokines and their associated signaling pathways on mesenchymal stem cell migration after spinal cord injury, to determine their roles in the curative effects of mesenchymal stem cells.This study reviewed the effects of tumor necrosis factor-α, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor, basic fibroblast growth factor, insulin like growth factor-1, stromal cell-derived factor and monocyte chemoattractant protein-1, 3 during mesenchymal stem cell migration to damaged sites, and analyzed the signal transduction pathways involved in their effects on mesenchymal stem cell migration.The results confirmed that phosphatidylinositol 3-kinase/serine/threonine protein kinases and nuclear factor-κB play crucial roles in the migration of mesenchymal stem cells induced by cytokines and chemokines.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.

ABSTRACT
We investigated the effects of cytokines and chemokines and their associated signaling pathways on mesenchymal stem cell migration after spinal cord injury, to determine their roles in the curative effects of mesenchymal stem cells. This study reviewed the effects of tumor necrosis factor-α, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor, basic fibroblast growth factor, insulin like growth factor-1, stromal cell-derived factor and monocyte chemoattractant protein-1, 3 during mesenchymal stem cell migration to damaged sites, and analyzed the signal transduction pathways involved in their effects on mesenchymal stem cell migration. The results confirmed that phosphatidylinositol 3-kinase/serine/threonine protein kinases and nuclear factor-κB play crucial roles in the migration of mesenchymal stem cells induced by cytokines and chemokines.

No MeSH data available.


Related in: MedlinePlus

Schematic diagram of cytokine pathways.Cytokines activated signaling cascade reactions in mesenchymal stem cells (MSCs) via receptor tyrosine kinase, and promoted cell migration.The combination of cytokines and RTK activated the guanine nucleotide-exchange factor Sos, and further activated the Ras/PI3K/Akt, Ras/MEK/ERK, and Ras/’Rac/PAK pathways, activated ERK, JNK and p38 signaling molecules, accelerated gene expression and protein synthesis, and promoted cell migration ability.The signaling cascade reactions were interlinked, and showed complementation and interdependence. Any deletion would thus affect cytokine-mediated MSC migration ability.PDK: 3-phosphoinositide-dependent protein kinase; ERK: extracellular signal-regulated kinase; JNK: c-Jun NH2-terminal kinases; PAK: p21-activated kinase; PI3K: phosphatidylinositol-3 kinase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4340025&req=5

Figure 1: Schematic diagram of cytokine pathways.Cytokines activated signaling cascade reactions in mesenchymal stem cells (MSCs) via receptor tyrosine kinase, and promoted cell migration.The combination of cytokines and RTK activated the guanine nucleotide-exchange factor Sos, and further activated the Ras/PI3K/Akt, Ras/MEK/ERK, and Ras/’Rac/PAK pathways, activated ERK, JNK and p38 signaling molecules, accelerated gene expression and protein synthesis, and promoted cell migration ability.The signaling cascade reactions were interlinked, and showed complementation and interdependence. Any deletion would thus affect cytokine-mediated MSC migration ability.PDK: 3-phosphoinositide-dependent protein kinase; ERK: extracellular signal-regulated kinase; JNK: c-Jun NH2-terminal kinases; PAK: p21-activated kinase; PI3K: phosphatidylinositol-3 kinase.

Mentions: Second, as shown in Figure 1, the chemotaxis induced by many cytokines and chemokines was confirmed by PI3K/Akt pathway experiments. Interactions between ligands and receptors could directly or indirectly trigger PI3K and further activate downstream signaling molecules, including Akt and protein kinase C, as well as the Rho family member Rac, resulting in cascade reactions in the cytoplasm and nucleus, and mediating MSC activity[18222526353641425256]. The PI3K/Rac pathway is important[2226294256]. The regulation by Rac of the downstream signaling molecules extracellular signal-regulated kinase and protein kinase C and the c-Jun NH2-terminal kinase pathway could induce nuclear gene expression and protein synthesis, resulting in MSC migration. It is assumed that the factors that trigger the PI3K/Akt pathway could activate NF-κB and control MSC migration activity.


Effects of cytokines and chemokines on migration of mesenchymal stem cells following spinal cord injury.

Li L, Yang M, Wang C, Zhao Q, Liu J, Zhan C, Liu Z, Li X, Wang W, Yang X - Neural Regen Res (2012)

Schematic diagram of cytokine pathways.Cytokines activated signaling cascade reactions in mesenchymal stem cells (MSCs) via receptor tyrosine kinase, and promoted cell migration.The combination of cytokines and RTK activated the guanine nucleotide-exchange factor Sos, and further activated the Ras/PI3K/Akt, Ras/MEK/ERK, and Ras/’Rac/PAK pathways, activated ERK, JNK and p38 signaling molecules, accelerated gene expression and protein synthesis, and promoted cell migration ability.The signaling cascade reactions were interlinked, and showed complementation and interdependence. Any deletion would thus affect cytokine-mediated MSC migration ability.PDK: 3-phosphoinositide-dependent protein kinase; ERK: extracellular signal-regulated kinase; JNK: c-Jun NH2-terminal kinases; PAK: p21-activated kinase; PI3K: phosphatidylinositol-3 kinase.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4340025&req=5

Figure 1: Schematic diagram of cytokine pathways.Cytokines activated signaling cascade reactions in mesenchymal stem cells (MSCs) via receptor tyrosine kinase, and promoted cell migration.The combination of cytokines and RTK activated the guanine nucleotide-exchange factor Sos, and further activated the Ras/PI3K/Akt, Ras/MEK/ERK, and Ras/’Rac/PAK pathways, activated ERK, JNK and p38 signaling molecules, accelerated gene expression and protein synthesis, and promoted cell migration ability.The signaling cascade reactions were interlinked, and showed complementation and interdependence. Any deletion would thus affect cytokine-mediated MSC migration ability.PDK: 3-phosphoinositide-dependent protein kinase; ERK: extracellular signal-regulated kinase; JNK: c-Jun NH2-terminal kinases; PAK: p21-activated kinase; PI3K: phosphatidylinositol-3 kinase.
Mentions: Second, as shown in Figure 1, the chemotaxis induced by many cytokines and chemokines was confirmed by PI3K/Akt pathway experiments. Interactions between ligands and receptors could directly or indirectly trigger PI3K and further activate downstream signaling molecules, including Akt and protein kinase C, as well as the Rho family member Rac, resulting in cascade reactions in the cytoplasm and nucleus, and mediating MSC activity[18222526353641425256]. The PI3K/Rac pathway is important[2226294256]. The regulation by Rac of the downstream signaling molecules extracellular signal-regulated kinase and protein kinase C and the c-Jun NH2-terminal kinase pathway could induce nuclear gene expression and protein synthesis, resulting in MSC migration. It is assumed that the factors that trigger the PI3K/Akt pathway could activate NF-κB and control MSC migration activity.

Bottom Line: We investigated the effects of cytokines and chemokines and their associated signaling pathways on mesenchymal stem cell migration after spinal cord injury, to determine their roles in the curative effects of mesenchymal stem cells.This study reviewed the effects of tumor necrosis factor-α, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor, basic fibroblast growth factor, insulin like growth factor-1, stromal cell-derived factor and monocyte chemoattractant protein-1, 3 during mesenchymal stem cell migration to damaged sites, and analyzed the signal transduction pathways involved in their effects on mesenchymal stem cell migration.The results confirmed that phosphatidylinositol 3-kinase/serine/threonine protein kinases and nuclear factor-κB play crucial roles in the migration of mesenchymal stem cells induced by cytokines and chemokines.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.

ABSTRACT
We investigated the effects of cytokines and chemokines and their associated signaling pathways on mesenchymal stem cell migration after spinal cord injury, to determine their roles in the curative effects of mesenchymal stem cells. This study reviewed the effects of tumor necrosis factor-α, vascular endothelial growth factor, hepatocyte growth factor, platelet-derived growth factor, basic fibroblast growth factor, insulin like growth factor-1, stromal cell-derived factor and monocyte chemoattractant protein-1, 3 during mesenchymal stem cell migration to damaged sites, and analyzed the signal transduction pathways involved in their effects on mesenchymal stem cell migration. The results confirmed that phosphatidylinositol 3-kinase/serine/threonine protein kinases and nuclear factor-κB play crucial roles in the migration of mesenchymal stem cells induced by cytokines and chemokines.

No MeSH data available.


Related in: MedlinePlus