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Effects of exogenous gibberellic acid3 on iron and manganese plaque amounts and iron and manganese uptake in rice.

Guo Y, Zhu C, Gan L, Ng D, Xia K - PLoS ONE (2015)

Bottom Line: Gibberellins (GA) regulate various components of plant development.Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.).In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Gibberellins (GA) regulate various components of plant development. Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.). In this study, we found that exogenous gibberellic acid3 (GA3) spray decreased Fe plaque, but increased Mn plaque, with applications of Kimura B nutrient solution. Similar effects from GA3, leading to reduced Fe plaque and increased Mn plaque, were also found by scanning electron microscopy and energy dispersive X-ray spectrometric microanalysis. Reduced Fe plaque was observed after applying GA3 to the groups containing added Fe2+ (17 and 42 mg•L(-1)) and an increasing trend was detected in Mn plaques of the Mn2+ (34 and 84 mg•L(-1)) added treatments. In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3. The uptake of Fe or Mn in rice plants was enhanced after GA3 application and Fe or Mn plaque production. Strong synergetic effects of GA3 application on Fe plaque production were detected. However, no synergetic effects on Mn plaque production were detected.

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Effects of induction by application of exogenous plant hormones and metal ions on Fe and Mn plaque production.Four-leaf rice seedlings grown in KB (1.2 mg·L-1 Fe2+ and 0.5 mg·L-1 Mn2+, pH 5.0) and KB containing various concentrations of Fe2+ (zero Fe, 17 mg·L-1 and 42 mg·L-1, pH 5.0) or Mn2+ (zero Mn, 34 mg·L-1 and 84 mg·L-1, pH 5.0) were harvested at 60 h after spraying with 0.18 mM exogenous GA3 or 0.12 mM exogenous S3307. Plants that did not undergo GA3 or S3307 treatment served as the control. The Fe (A) and Mn (B) plaques were extracted using the DCB method and detected by ICP-OES. Data are the means ± SE of at least three independent experiments (n = 15) with similar results. The control and exogenous GA3 or S3307 treatments were compared using a t-test.
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pone.0118177.g004: Effects of induction by application of exogenous plant hormones and metal ions on Fe and Mn plaque production.Four-leaf rice seedlings grown in KB (1.2 mg·L-1 Fe2+ and 0.5 mg·L-1 Mn2+, pH 5.0) and KB containing various concentrations of Fe2+ (zero Fe, 17 mg·L-1 and 42 mg·L-1, pH 5.0) or Mn2+ (zero Mn, 34 mg·L-1 and 84 mg·L-1, pH 5.0) were harvested at 60 h after spraying with 0.18 mM exogenous GA3 or 0.12 mM exogenous S3307. Plants that did not undergo GA3 or S3307 treatment served as the control. The Fe (A) and Mn (B) plaques were extracted using the DCB method and detected by ICP-OES. Data are the means ± SE of at least three independent experiments (n = 15) with similar results. The control and exogenous GA3 or S3307 treatments were compared using a t-test.

Mentions: There was a dose-dependent increase in Fe plaque content in response to high Fe2+-inducing treatments of exogenous (NH4)2SO4·FeSO4·6H2O, compared to the KB and zero Fe treatments (Fig. 4). The same tendency was found for Mn2+-inducing treatments with exogenous MnSO4·H2O (Fig. 4), which is consistent with a previous report [38]. Compared to the control, GA3 treatments significantly decreased Fe plaque content at 17 and 42 mg·L-1 Fe ion (Fig. 4A).


Effects of exogenous gibberellic acid3 on iron and manganese plaque amounts and iron and manganese uptake in rice.

Guo Y, Zhu C, Gan L, Ng D, Xia K - PLoS ONE (2015)

Effects of induction by application of exogenous plant hormones and metal ions on Fe and Mn plaque production.Four-leaf rice seedlings grown in KB (1.2 mg·L-1 Fe2+ and 0.5 mg·L-1 Mn2+, pH 5.0) and KB containing various concentrations of Fe2+ (zero Fe, 17 mg·L-1 and 42 mg·L-1, pH 5.0) or Mn2+ (zero Mn, 34 mg·L-1 and 84 mg·L-1, pH 5.0) were harvested at 60 h after spraying with 0.18 mM exogenous GA3 or 0.12 mM exogenous S3307. Plants that did not undergo GA3 or S3307 treatment served as the control. The Fe (A) and Mn (B) plaques were extracted using the DCB method and detected by ICP-OES. Data are the means ± SE of at least three independent experiments (n = 15) with similar results. The control and exogenous GA3 or S3307 treatments were compared using a t-test.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4339979&req=5

pone.0118177.g004: Effects of induction by application of exogenous plant hormones and metal ions on Fe and Mn plaque production.Four-leaf rice seedlings grown in KB (1.2 mg·L-1 Fe2+ and 0.5 mg·L-1 Mn2+, pH 5.0) and KB containing various concentrations of Fe2+ (zero Fe, 17 mg·L-1 and 42 mg·L-1, pH 5.0) or Mn2+ (zero Mn, 34 mg·L-1 and 84 mg·L-1, pH 5.0) were harvested at 60 h after spraying with 0.18 mM exogenous GA3 or 0.12 mM exogenous S3307. Plants that did not undergo GA3 or S3307 treatment served as the control. The Fe (A) and Mn (B) plaques were extracted using the DCB method and detected by ICP-OES. Data are the means ± SE of at least three independent experiments (n = 15) with similar results. The control and exogenous GA3 or S3307 treatments were compared using a t-test.
Mentions: There was a dose-dependent increase in Fe plaque content in response to high Fe2+-inducing treatments of exogenous (NH4)2SO4·FeSO4·6H2O, compared to the KB and zero Fe treatments (Fig. 4). The same tendency was found for Mn2+-inducing treatments with exogenous MnSO4·H2O (Fig. 4), which is consistent with a previous report [38]. Compared to the control, GA3 treatments significantly decreased Fe plaque content at 17 and 42 mg·L-1 Fe ion (Fig. 4A).

Bottom Line: Gibberellins (GA) regulate various components of plant development.Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.).In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China.

ABSTRACT
Gibberellins (GA) regulate various components of plant development. Iron and Mn plaque result from oxiding and hydroxiding Fe and Mn, respectively, on the roots of aquatic plant species such as rice (Oryza sativa L.). In this study, we found that exogenous gibberellic acid3 (GA3) spray decreased Fe plaque, but increased Mn plaque, with applications of Kimura B nutrient solution. Similar effects from GA3, leading to reduced Fe plaque and increased Mn plaque, were also found by scanning electron microscopy and energy dispersive X-ray spectrometric microanalysis. Reduced Fe plaque was observed after applying GA3 to the groups containing added Fe2+ (17 and 42 mg•L(-1)) and an increasing trend was detected in Mn plaques of the Mn2+ (34 and 84 mg•L(-1)) added treatments. In contrast, an inhibitor of GA3, uniconazole, reversed the effects of GA3. The uptake of Fe or Mn in rice plants was enhanced after GA3 application and Fe or Mn plaque production. Strong synergetic effects of GA3 application on Fe plaque production were detected. However, no synergetic effects on Mn plaque production were detected.

Show MeSH
Related in: MedlinePlus