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Circulating thrombopoietin levels in normal healthy blood donors and in aplastic anemia patients in relation to disease severity.

Singh A, Verma A, Nityanand S, Chaudhary R, Elhence P - Asian J Transfus Sci (2015 Jan-Jun)

Bottom Line: No significant difference was observed in TPO levels of male and female patients (P = 0.453).The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml), 1190 pg/ml (range 672.1-7651 pg/ml), and 1111.5 pg/ml (range 761.1-2289.2 pg/ml), respectively.TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043), with no significant relation among rest of the groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Background: Thrombopoietin (TPO) is the key hematopoietic growth factor regulating the production of platelets from bone marrow megakaryocytes and maintaining platelet hemostasis. This study was done to find any relationship between the levels of thrombopoietin and the severity of disease in patients with aplastic anemia.

Materials and methods: Serum samples were collected from 52 patients with a confirmed diagnosis of aplastic anemia and 45 normal healthy blood donors of both sexes over a period of 2 years, and TPO was estimated by using commercially available TPO-specific-enzyme-linked immunosorbent assay.

Results: The median TPO level of 1190 pg/ml (range 625-7651 pg/ml) in aplastic anemia patients was significantly higher than the median TPO level of 121.1 pg/ml (81.25-237.7 pg/ml) in normal healthy blood donors (P = 0.000). No significant difference was observed in TPO levels of male and female patients (P = 0.453). The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml), 1190 pg/ml (range 672.1-7651 pg/ml), and 1111.5 pg/ml (range 761.1-2289.2 pg/ml), respectively. TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043), with no significant relation among rest of the groups.

Discussion: TPO levels in aplastic anemia patients were significantly higher than in healthy blood donors; however, in aplastic anemia patients TPO levels were significantly higher only in patients with very severe disease.

No MeSH data available.


Related in: MedlinePlus

Linear regression analysis with 95% confidence interval indicated by upper and lower curved lines, with each dot representing normal healthy blood donor with their platelet counts corresponding to x-axis and TPO levels to y-axis
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Figure 4: Linear regression analysis with 95% confidence interval indicated by upper and lower curved lines, with each dot representing normal healthy blood donor with their platelet counts corresponding to x-axis and TPO levels to y-axis

Mentions: Linear regression analysis with 95% confidence interval, between serum TPO levels and platelet counts in AA patients and normal healthy blood donors, showed no clinically significant correlation (r2 = 0.002), and ANOVA P value = 0.731 and 0.777, respectively [Figures 3 and 4].


Circulating thrombopoietin levels in normal healthy blood donors and in aplastic anemia patients in relation to disease severity.

Singh A, Verma A, Nityanand S, Chaudhary R, Elhence P - Asian J Transfus Sci (2015 Jan-Jun)

Linear regression analysis with 95% confidence interval indicated by upper and lower curved lines, with each dot representing normal healthy blood donor with their platelet counts corresponding to x-axis and TPO levels to y-axis
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4339937&req=5

Figure 4: Linear regression analysis with 95% confidence interval indicated by upper and lower curved lines, with each dot representing normal healthy blood donor with their platelet counts corresponding to x-axis and TPO levels to y-axis
Mentions: Linear regression analysis with 95% confidence interval, between serum TPO levels and platelet counts in AA patients and normal healthy blood donors, showed no clinically significant correlation (r2 = 0.002), and ANOVA P value = 0.731 and 0.777, respectively [Figures 3 and 4].

Bottom Line: No significant difference was observed in TPO levels of male and female patients (P = 0.453).The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml), 1190 pg/ml (range 672.1-7651 pg/ml), and 1111.5 pg/ml (range 761.1-2289.2 pg/ml), respectively.TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043), with no significant relation among rest of the groups.

View Article: PubMed Central - PubMed

Affiliation: Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.

ABSTRACT

Background: Thrombopoietin (TPO) is the key hematopoietic growth factor regulating the production of platelets from bone marrow megakaryocytes and maintaining platelet hemostasis. This study was done to find any relationship between the levels of thrombopoietin and the severity of disease in patients with aplastic anemia.

Materials and methods: Serum samples were collected from 52 patients with a confirmed diagnosis of aplastic anemia and 45 normal healthy blood donors of both sexes over a period of 2 years, and TPO was estimated by using commercially available TPO-specific-enzyme-linked immunosorbent assay.

Results: The median TPO level of 1190 pg/ml (range 625-7651 pg/ml) in aplastic anemia patients was significantly higher than the median TPO level of 121.1 pg/ml (81.25-237.7 pg/ml) in normal healthy blood donors (P = 0.000). No significant difference was observed in TPO levels of male and female patients (P = 0.453). The median TPO concentrations observed in very severe aplastic anemia, severe aplastic anemia, and nonsevere aplastic anemia were 2765 pg/ml (range 625-6451 pg/ml), 1190 pg/ml (range 672.1-7651 pg/ml), and 1111.5 pg/ml (range 761.1-2289.2 pg/ml), respectively. TPO in patients of very severe aplastic anemia was significantly higher than patients of nonsevere aplastic anemia (P = 0.043), with no significant relation among rest of the groups.

Discussion: TPO levels in aplastic anemia patients were significantly higher than in healthy blood donors; however, in aplastic anemia patients TPO levels were significantly higher only in patients with very severe disease.

No MeSH data available.


Related in: MedlinePlus