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Resistance to and accumulation of heavy metals by actinobacteria isolated from abandoned mining areas.

El Baz S, Baz M, Barakate M, Hassani L, El Gharmali A, Imziln B - ScientificWorldJournal (2015)

Bottom Line: Copper "Cu," cadmium "Cd," and chromium "Cr" were found with moderate and low concentrations.Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera.BN3).

View Article: PubMed Central - PubMed

Affiliation: Environmental Microbiology and Toxicology Unit, Laboratory of Biology and Biotechnology of Microorganisms (LBBM), Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000 Marrakech, Morocco.

ABSTRACT
Accumulation of high concentrations of heavy metals in environments can cause many human health risks and serious ecological problems. Nowadays, bioremediation using microorganisms is receiving much attention due to their good performance. The aim of this work is to investigate heavy metals resistance and bioaccumulation potential of actinobacteria strains isolated from some abandoned mining areas. Analysis of mining residues revealed that high concentration of zinc "Zn" was recorded in Sidi Bouatman, Arbar, and Bir Nhass mining residues. The highest concentration of lead "Pb" was found in Sidi Bouatman. Copper "Cu," cadmium "Cd," and chromium "Cr" were found with moderate and low concentrations. The resistance of 59 isolated actinobacteria to the five heavy metals was also determined. Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera. The results showed different levels of heavy metal resistance; the minimum inhibitory concentration (MIC) recorded was 0.55 for Pb, 0.15 for Cr, and 0.10 mg·mL(-1) for both Zn and Cu. Chemical precipitation assay of heavy metals using hydrogen sulfide technic (H2S) revealed that only 27 isolates have a strong ability to accumulate Pb (up to 600 mg of Pb per g of biomass for Streptomyces sp. BN3).

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Localisation of prospected abandoned mining areas of Marrakech, Morocco: Kettara, Sidi bouatman, Bir Nhass, and Goundafa.
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Related In: Results  -  Collection


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fig1: Localisation of prospected abandoned mining areas of Marrakech, Morocco: Kettara, Sidi bouatman, Bir Nhass, and Goundafa.

Mentions: In the present study we are interested in mining residues collected from various abandoned mining areas located around Marrakech region, West Central Morocco (Figure 1). Kettara mine (KT) is located approximately 30 km North-West of Marrakech and was operated for pyrrhotine extraction until 1981 [22]. Sidi Bouatman mine (SB) is located approximately 33 km north of Marrakech and was operated for Pb, Zn, and pyrite extraction until 1980. The old Bir Nhass zinc mine (BN) is located approximately 30 km north of Marrakech; it was closed at the end of the 20th century. Goundafa mine, located approximately at 90 km south of Marrakech, operated for Pb, Zn, and Cu extraction and in 1989 the mine has ceased to be functional. In Goundafa area, samples were collected from two sites Arbar plant (GA) and Tenfit Mine (GT).


Resistance to and accumulation of heavy metals by actinobacteria isolated from abandoned mining areas.

El Baz S, Baz M, Barakate M, Hassani L, El Gharmali A, Imziln B - ScientificWorldJournal (2015)

Localisation of prospected abandoned mining areas of Marrakech, Morocco: Kettara, Sidi bouatman, Bir Nhass, and Goundafa.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4339716&req=5

fig1: Localisation of prospected abandoned mining areas of Marrakech, Morocco: Kettara, Sidi bouatman, Bir Nhass, and Goundafa.
Mentions: In the present study we are interested in mining residues collected from various abandoned mining areas located around Marrakech region, West Central Morocco (Figure 1). Kettara mine (KT) is located approximately 30 km North-West of Marrakech and was operated for pyrrhotine extraction until 1981 [22]. Sidi Bouatman mine (SB) is located approximately 33 km north of Marrakech and was operated for Pb, Zn, and pyrite extraction until 1980. The old Bir Nhass zinc mine (BN) is located approximately 30 km north of Marrakech; it was closed at the end of the 20th century. Goundafa mine, located approximately at 90 km south of Marrakech, operated for Pb, Zn, and Cu extraction and in 1989 the mine has ceased to be functional. In Goundafa area, samples were collected from two sites Arbar plant (GA) and Tenfit Mine (GT).

Bottom Line: Copper "Cu," cadmium "Cd," and chromium "Cr" were found with moderate and low concentrations.Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera.BN3).

View Article: PubMed Central - PubMed

Affiliation: Environmental Microbiology and Toxicology Unit, Laboratory of Biology and Biotechnology of Microorganisms (LBBM), Faculty of Sciences Semlalia, Cadi Ayyad University, P.O. Box 2390, 40000 Marrakech, Morocco.

ABSTRACT
Accumulation of high concentrations of heavy metals in environments can cause many human health risks and serious ecological problems. Nowadays, bioremediation using microorganisms is receiving much attention due to their good performance. The aim of this work is to investigate heavy metals resistance and bioaccumulation potential of actinobacteria strains isolated from some abandoned mining areas. Analysis of mining residues revealed that high concentration of zinc "Zn" was recorded in Sidi Bouatman, Arbar, and Bir Nhass mining residues. The highest concentration of lead "Pb" was found in Sidi Bouatman. Copper "Cu," cadmium "Cd," and chromium "Cr" were found with moderate and low concentrations. The resistance of 59 isolated actinobacteria to the five heavy metals was also determined. Using molecular identification 16S rRNA, these 27 isolates were found to belong to Streptomyces and Amycolatopsis genera. The results showed different levels of heavy metal resistance; the minimum inhibitory concentration (MIC) recorded was 0.55 for Pb, 0.15 for Cr, and 0.10 mg·mL(-1) for both Zn and Cu. Chemical precipitation assay of heavy metals using hydrogen sulfide technic (H2S) revealed that only 27 isolates have a strong ability to accumulate Pb (up to 600 mg of Pb per g of biomass for Streptomyces sp. BN3).

Show MeSH