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Relaxant action of plumula nelumbinis extract on mouse airway smooth muscle.

Tan L, Chen W, Wei MY, Shen J, Yu MF, Yang G, Guo D, Qin G, Ji G, Liu QH - Evid Based Complement Alternat Med (2015)

Bottom Line: However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated.Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents.This finding indicates that CEPN would be a candidate of new potent bronchodilators.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasmin Wuling Area of China, College of Life Sciences, South Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
The traditional herb Plumula Nelumbinis is widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract of Plumula Nelumbinis (CEPN) was prepared, which completely inhibited precontraction induced by high K(+) in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca(2+) channel- (VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca(2+) influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.

No MeSH data available.


Related in: MedlinePlus

CEPN blocks high K+-evoked Ca2+ influx. (a) A representative tracing of 4 experiments. Under Ca2+-free conditions (0 Ca2+ and 0.5 mM EGTA), high K+ did not evoke contraction in a tracheal ring. After the restoration of 2 mM Ca2+, a sustained contraction occurred, which was fully inhibited by CEPN. (b) In the presence of 1 mg/mL CEPN, the identical experiments as above were performed. A Ca2+ restoration-provoked contraction was not noted. This experiment was conducted in 6 tracheal rings. These results suggest that CEPN inhibits Ca2+ influx.
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fig2: CEPN blocks high K+-evoked Ca2+ influx. (a) A representative tracing of 4 experiments. Under Ca2+-free conditions (0 Ca2+ and 0.5 mM EGTA), high K+ did not evoke contraction in a tracheal ring. After the restoration of 2 mM Ca2+, a sustained contraction occurred, which was fully inhibited by CEPN. (b) In the presence of 1 mg/mL CEPN, the identical experiments as above were performed. A Ca2+ restoration-provoked contraction was not noted. This experiment was conducted in 6 tracheal rings. These results suggest that CEPN inhibits Ca2+ influx.

Mentions: The above results suggest that the relaxation induced by CEPN might be due to the termination of VDCC-mediated Ca2+ influx. This hypothesis was examined in the following experiments. Figure 2(a) shows that high K+ did not induce contraction under Ca2+-free conditions (0 mM Ca2+ and 0.5 mM EGTA); however, contraction immediately occurred following Ca2+ restoration (2 mM), and CEPN (1 mg/mL) completely inhibited contraction. However, a Ca2+ restoration-induced contraction was not observed in the presence of 1 mg/mL CEPN (Figure 2(b)). This result suggests that CEPN-evoked relaxation of high K+-induced precontraction was completely dependent on the inhibition on VDCC-mediated Ca2+ influx.


Relaxant action of plumula nelumbinis extract on mouse airway smooth muscle.

Tan L, Chen W, Wei MY, Shen J, Yu MF, Yang G, Guo D, Qin G, Ji G, Liu QH - Evid Based Complement Alternat Med (2015)

CEPN blocks high K+-evoked Ca2+ influx. (a) A representative tracing of 4 experiments. Under Ca2+-free conditions (0 Ca2+ and 0.5 mM EGTA), high K+ did not evoke contraction in a tracheal ring. After the restoration of 2 mM Ca2+, a sustained contraction occurred, which was fully inhibited by CEPN. (b) In the presence of 1 mg/mL CEPN, the identical experiments as above were performed. A Ca2+ restoration-provoked contraction was not noted. This experiment was conducted in 6 tracheal rings. These results suggest that CEPN inhibits Ca2+ influx.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4339714&req=5

fig2: CEPN blocks high K+-evoked Ca2+ influx. (a) A representative tracing of 4 experiments. Under Ca2+-free conditions (0 Ca2+ and 0.5 mM EGTA), high K+ did not evoke contraction in a tracheal ring. After the restoration of 2 mM Ca2+, a sustained contraction occurred, which was fully inhibited by CEPN. (b) In the presence of 1 mg/mL CEPN, the identical experiments as above were performed. A Ca2+ restoration-provoked contraction was not noted. This experiment was conducted in 6 tracheal rings. These results suggest that CEPN inhibits Ca2+ influx.
Mentions: The above results suggest that the relaxation induced by CEPN might be due to the termination of VDCC-mediated Ca2+ influx. This hypothesis was examined in the following experiments. Figure 2(a) shows that high K+ did not induce contraction under Ca2+-free conditions (0 mM Ca2+ and 0.5 mM EGTA); however, contraction immediately occurred following Ca2+ restoration (2 mM), and CEPN (1 mg/mL) completely inhibited contraction. However, a Ca2+ restoration-induced contraction was not observed in the presence of 1 mg/mL CEPN (Figure 2(b)). This result suggests that CEPN-evoked relaxation of high K+-induced precontraction was completely dependent on the inhibition on VDCC-mediated Ca2+ influx.

Bottom Line: However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated.Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents.This finding indicates that CEPN would be a candidate of new potent bronchodilators.

View Article: PubMed Central - PubMed

Affiliation: Institute for Medical Biology & Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasmin Wuling Area of China, College of Life Sciences, South Central University for Nationalities, Wuhan 430074, China.

ABSTRACT
The traditional herb Plumula Nelumbinis is widely used in the world because it has many biological activities, such as anti-inflammation, antioxidant, antihypertension, and butyrylcholinesterase inhibition. However, the action of Plumula Nelumbinis on airway smooth muscle (ASM) relaxation has not been investigated. A chloroform extract of Plumula Nelumbinis (CEPN) was prepared, which completely inhibited precontraction induced by high K(+) in a concentration-dependent manner in mouse tracheal rings, but it had no effect on resting tension. CEPN also blocked voltage-dependent L-type Ca(2+) channel- (VDCC-) mediated currents. In addition, ACh-induced precontraction was also completely blocked by CEPN and partially inhibited by nifedipine or pyrazole 3. Besides, CEPN partially reduced ACh-activated nonselective cation channel (NSCC) currents. Taken together, our data demonstrate that CEPN blocked VDCC and NSCC to inhibit Ca(2+) influx, resulting in relaxation of precontracted ASM. This finding indicates that CEPN would be a candidate of new potent bronchodilators.

No MeSH data available.


Related in: MedlinePlus