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Hypertension is associated with preamyloid oligomers in human atrium: a missing link in atrial pathophysiology?

Sidorova TN, Mace LC, Wells KS, Yermalitskaya LV, Su PF, Shyr Y, Atkinson JB, Fogo AB, Prinsen JK, Byrne JG, Petracek MR, Greelish JP, Hoff SJ, Ball SK, Glabe CG, Brown NJ, Barnett JV, Murray KT - J Am Heart Assoc (2014)

Bottom Line: Increasing evidence indicates that proteotoxicity plays a pathophysiologic role in experimental and human cardiomyopathy.A majority (n=62) underwent aortic valve replacement, with fewer undergoing coronary artery bypass grafting (n=34) or mitral valve replacement/repair (n=24).PAOs are frequently detected in human atrium, where their presence is associated with clinical hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, TN (T.N.S., L.C.M., L.V.Y., J.K.P., N.J.B., J.V.B., K.T.M.).

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Representative histologic features in atrial samples from patients with low, medium, and high green/red ratio (G/R) values. For each patient sample (rows), the columns from left to right display images showing: hematoxylin and eosin–stained section (A); Congo red–stained section with visualization using polarized light (B); and Masson's trichrome–stained section demonstrating collagen (blue) representing areas of fibrosis (C). The G/R values are shown to the left of the images.
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fig03: Representative histologic features in atrial samples from patients with low, medium, and high green/red ratio (G/R) values. For each patient sample (rows), the columns from left to right display images showing: hematoxylin and eosin–stained section (A); Congo red–stained section with visualization using polarized light (B); and Masson's trichrome–stained section demonstrating collagen (blue) representing areas of fibrosis (C). The G/R values are shown to the left of the images.

Mentions: To ensure that the A‐11 antibody was detecting protein oligomers and not amyloid fibrils, Congo red staining was performed on 16 patient samples representing a range of mean G/R values (0.03±0.02 to 0.43±0.06). Examination of all Congo red–stained sections under polarized light revealed no evidence of amyloid deposition (Figure 3B). These results further confirm that the protein oligomers identified in the atrial samples are not mature amyloid, given the negative Congo red staining.


Hypertension is associated with preamyloid oligomers in human atrium: a missing link in atrial pathophysiology?

Sidorova TN, Mace LC, Wells KS, Yermalitskaya LV, Su PF, Shyr Y, Atkinson JB, Fogo AB, Prinsen JK, Byrne JG, Petracek MR, Greelish JP, Hoff SJ, Ball SK, Glabe CG, Brown NJ, Barnett JV, Murray KT - J Am Heart Assoc (2014)

Representative histologic features in atrial samples from patients with low, medium, and high green/red ratio (G/R) values. For each patient sample (rows), the columns from left to right display images showing: hematoxylin and eosin–stained section (A); Congo red–stained section with visualization using polarized light (B); and Masson's trichrome–stained section demonstrating collagen (blue) representing areas of fibrosis (C). The G/R values are shown to the left of the images.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4338732&req=5

fig03: Representative histologic features in atrial samples from patients with low, medium, and high green/red ratio (G/R) values. For each patient sample (rows), the columns from left to right display images showing: hematoxylin and eosin–stained section (A); Congo red–stained section with visualization using polarized light (B); and Masson's trichrome–stained section demonstrating collagen (blue) representing areas of fibrosis (C). The G/R values are shown to the left of the images.
Mentions: To ensure that the A‐11 antibody was detecting protein oligomers and not amyloid fibrils, Congo red staining was performed on 16 patient samples representing a range of mean G/R values (0.03±0.02 to 0.43±0.06). Examination of all Congo red–stained sections under polarized light revealed no evidence of amyloid deposition (Figure 3B). These results further confirm that the protein oligomers identified in the atrial samples are not mature amyloid, given the negative Congo red staining.

Bottom Line: Increasing evidence indicates that proteotoxicity plays a pathophysiologic role in experimental and human cardiomyopathy.A majority (n=62) underwent aortic valve replacement, with fewer undergoing coronary artery bypass grafting (n=34) or mitral valve replacement/repair (n=24).PAOs are frequently detected in human atrium, where their presence is associated with clinical hypertension.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Pharmacology, Vanderbilt University School of Medicine, Nashville, TN (T.N.S., L.C.M., L.V.Y., J.K.P., N.J.B., J.V.B., K.T.M.).

Show MeSH
Related in: MedlinePlus