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Do all antioxidant supplements have the same potential effect on preventing myringosclerosis?

Akdagli S, Tuzuner A, Demirci S, Karadas H, Tulaci KG, Dogan M, Caylan R - Clin Exp Otorhinolaryngol (2015)

Bottom Line: Myringosclerosis is an irreversible pathological healing mechanism of the tympanic membrane which can result in the formation of sclerotic plaques.All tympanic membrane lesions were evaluated and compared otomicroscopically and histopathologically.CoQ10, lycopene, and GSE were compared against a saline group and their antioxidative and anti-inflammatory effects were similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Ministry of Health, Etlik İhtisas Training and Research Hospital, Ankara, Turkey.

ABSTRACT

Objectives: Myringosclerosis is an irreversible pathological healing mechanism of the tympanic membrane which can result in the formation of sclerotic plaques. Antioxidant treatment is a recognised prevention therapy and coenzyme Q10 (CoQ10), lycopene, and grape seed extract (GSE), were used in this manner.

Methods: Forty-four Wistar rats were used in this experiment, and, following myringotomies, the animals were randomly divided into four groups. CoQ10, lycopene or GSE was administered orally to the respective groups, starting from the day of surgery. Otomicroscopy examination was performed on the 14th day. All tympanic membrane lesions were evaluated and compared otomicroscopically and histopathologically.

Results: The otomicroscopy and histopathological findings, compared against a control (saline) group, showed the CoQ10, lycopene, and GSE groups had statistically significant differences of degree of sclerosis (P<0.001).

Conclusion: CoQ10, lycopene, and GSE were compared against a saline group and their antioxidative and anti-inflammatory effects were similar. The formation of myringosclerotic plagues after experimental myringotomy in rats significantly decreased and diminished after systemic administration of the three different antioxidant supplements.

No MeSH data available.


Related in: MedlinePlus

Trichrome stain of the same rat in Fig. 4 (saline group, grade 3 sclerosis) shows marked fibrosis in lamina propria (arrow) (Masson's trichrome, ×100).
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Figure 5: Trichrome stain of the same rat in Fig. 4 (saline group, grade 3 sclerosis) shows marked fibrosis in lamina propria (arrow) (Masson's trichrome, ×100).

Mentions: All serial sections were examined with a light microscope (DP2-BSW, Olympus, Tokyo, Japan). The scoring of the lesions was done on the section that demonstrates the maximum fibrotic change for each rat. The degree of sclerotic lesions and intensity of fibroblastic proliferation in the lamina propria of timpanic membranes were graded semiquantitatively as: 0, no visible myringosclerosis, lamina propria was free of sclerotic lesion (Fig. 1); 1, occasional miringosclerosis, few single lesions in the lamina propria (Fig. 2); 2, moderate myringosclerosis, single and confluent lesions observed in the lamina propria (Fig. 3); 3, serious myringosclerosis, extensive confluent lesions observed in the lamina propria (Figs. 4, 5) [1].


Do all antioxidant supplements have the same potential effect on preventing myringosclerosis?

Akdagli S, Tuzuner A, Demirci S, Karadas H, Tulaci KG, Dogan M, Caylan R - Clin Exp Otorhinolaryngol (2015)

Trichrome stain of the same rat in Fig. 4 (saline group, grade 3 sclerosis) shows marked fibrosis in lamina propria (arrow) (Masson's trichrome, ×100).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4338085&req=5

Figure 5: Trichrome stain of the same rat in Fig. 4 (saline group, grade 3 sclerosis) shows marked fibrosis in lamina propria (arrow) (Masson's trichrome, ×100).
Mentions: All serial sections were examined with a light microscope (DP2-BSW, Olympus, Tokyo, Japan). The scoring of the lesions was done on the section that demonstrates the maximum fibrotic change for each rat. The degree of sclerotic lesions and intensity of fibroblastic proliferation in the lamina propria of timpanic membranes were graded semiquantitatively as: 0, no visible myringosclerosis, lamina propria was free of sclerotic lesion (Fig. 1); 1, occasional miringosclerosis, few single lesions in the lamina propria (Fig. 2); 2, moderate myringosclerosis, single and confluent lesions observed in the lamina propria (Fig. 3); 3, serious myringosclerosis, extensive confluent lesions observed in the lamina propria (Figs. 4, 5) [1].

Bottom Line: Myringosclerosis is an irreversible pathological healing mechanism of the tympanic membrane which can result in the formation of sclerotic plaques.All tympanic membrane lesions were evaluated and compared otomicroscopically and histopathologically.CoQ10, lycopene, and GSE were compared against a saline group and their antioxidative and anti-inflammatory effects were similar.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology-Head and Neck Surgery, Ministry of Health, Etlik İhtisas Training and Research Hospital, Ankara, Turkey.

ABSTRACT

Objectives: Myringosclerosis is an irreversible pathological healing mechanism of the tympanic membrane which can result in the formation of sclerotic plaques. Antioxidant treatment is a recognised prevention therapy and coenzyme Q10 (CoQ10), lycopene, and grape seed extract (GSE), were used in this manner.

Methods: Forty-four Wistar rats were used in this experiment, and, following myringotomies, the animals were randomly divided into four groups. CoQ10, lycopene or GSE was administered orally to the respective groups, starting from the day of surgery. Otomicroscopy examination was performed on the 14th day. All tympanic membrane lesions were evaluated and compared otomicroscopically and histopathologically.

Results: The otomicroscopy and histopathological findings, compared against a control (saline) group, showed the CoQ10, lycopene, and GSE groups had statistically significant differences of degree of sclerosis (P<0.001).

Conclusion: CoQ10, lycopene, and GSE were compared against a saline group and their antioxidative and anti-inflammatory effects were similar. The formation of myringosclerotic plagues after experimental myringotomy in rats significantly decreased and diminished after systemic administration of the three different antioxidant supplements.

No MeSH data available.


Related in: MedlinePlus