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Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma.

Guo XF, Yang ZR, Wang J, Lei XF, Lv XG, Dong WG - Mol Med Rep (2014)

Bottom Line: Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected.Results of the CCK‑8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments.In addition, it was determined that liver and renal toxicity did not increase in combined treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5‑fluorouracil (5‑FU) on BGC‑823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5‑FU alone or combined on BGC‑823 cells was determined using a cell counting kit 8 (CCK‑8) on living cells. Apoptotic morphological features and proteins expression levels were detected by Hoechst 33258 staining, an Annexin V/propidium iodide apoptosis kit and western blot analysis, respectively. Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected. Results of the CCK‑8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments. The tumor volume was 90.65% of that of the controls and the mean tumor weight was only 0.125 g at the end of the experiment in the combination group compared with the control group (0.822 g). In addition, it was determined that liver and renal toxicity did not increase in combined treatment. These findings showed that puerarin and 5‑FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects.

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Related in: MedlinePlus

(A) Dimensions and (B) weight of tumors at the end of the experiment. (C) Apoptotic cells were detected in xenograft tumor tissue using the TUNEL assay. Apoptotic cells appear brown and are indicated by arrows. Magnification, ×200. (D) Quantified results of the TUNEL assay. Values are presented as the mean ± standard deviation (n=6 per group). *P<0.05, compared with the puerarin+5-FU group; #P<0.05, compared with the control group. 5-FU, 5-fluorouracil; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
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f5-mmr-11-04-2562: (A) Dimensions and (B) weight of tumors at the end of the experiment. (C) Apoptotic cells were detected in xenograft tumor tissue using the TUNEL assay. Apoptotic cells appear brown and are indicated by arrows. Magnification, ×200. (D) Quantified results of the TUNEL assay. Values are presented as the mean ± standard deviation (n=6 per group). *P<0.05, compared with the puerarin+5-FU group; #P<0.05, compared with the control group. 5-FU, 5-fluorouracil; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.

Mentions: The effect of puerarin and 5-FU on the growth of primary tumor xenografts in nude mice was examined. Tumor volume was recorded every three days. The volumes of the tumors in the treatment groups were clearly reduced compared with those in the control group, while the inhibition rate in the combination group was 90.65%, which was a more significant inhibition than that in the other three groups (P<0.05; Table I). The data showed that the effect of the combined treatment was superior to the effect of puerarin or 5-FU individually. The mean tumor weight in the combination group was only 0.125 g at the end of the experiment compared with the control group (0.822 g) (Fig. 5A and B). TUNEL assays of the subcutaneous tumor tissue sections demonstrated that puerarin combined with 5-FU produced clear cell apoptosis in the tumor mass, while little apoptosis was observed in the control group (P<0.05, Fig. 5C and D).


Synergistic antitumor effect of puerarin combined with 5-fluorouracil on gastric carcinoma.

Guo XF, Yang ZR, Wang J, Lei XF, Lv XG, Dong WG - Mol Med Rep (2014)

(A) Dimensions and (B) weight of tumors at the end of the experiment. (C) Apoptotic cells were detected in xenograft tumor tissue using the TUNEL assay. Apoptotic cells appear brown and are indicated by arrows. Magnification, ×200. (D) Quantified results of the TUNEL assay. Values are presented as the mean ± standard deviation (n=6 per group). *P<0.05, compared with the puerarin+5-FU group; #P<0.05, compared with the control group. 5-FU, 5-fluorouracil; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4337718&req=5

f5-mmr-11-04-2562: (A) Dimensions and (B) weight of tumors at the end of the experiment. (C) Apoptotic cells were detected in xenograft tumor tissue using the TUNEL assay. Apoptotic cells appear brown and are indicated by arrows. Magnification, ×200. (D) Quantified results of the TUNEL assay. Values are presented as the mean ± standard deviation (n=6 per group). *P<0.05, compared with the puerarin+5-FU group; #P<0.05, compared with the control group. 5-FU, 5-fluorouracil; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling.
Mentions: The effect of puerarin and 5-FU on the growth of primary tumor xenografts in nude mice was examined. Tumor volume was recorded every three days. The volumes of the tumors in the treatment groups were clearly reduced compared with those in the control group, while the inhibition rate in the combination group was 90.65%, which was a more significant inhibition than that in the other three groups (P<0.05; Table I). The data showed that the effect of the combined treatment was superior to the effect of puerarin or 5-FU individually. The mean tumor weight in the combination group was only 0.125 g at the end of the experiment compared with the control group (0.822 g) (Fig. 5A and B). TUNEL assays of the subcutaneous tumor tissue sections demonstrated that puerarin combined with 5-FU produced clear cell apoptosis in the tumor mass, while little apoptosis was observed in the control group (P<0.05, Fig. 5C and D).

Bottom Line: Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected.Results of the CCK‑8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments.In addition, it was determined that liver and renal toxicity did not increase in combined treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Combination chemotherapy is a crucial method in the treatment of gastric cancer. The aim of the present study was to investigate the inhibitory effects of puerarin and 5‑fluorouracil (5‑FU) on BGC‑823 gastric cancer cells in vitro and in vivo. The in vitro growth inhibition of puerarin or 5‑FU alone or combined on BGC‑823 cells was determined using a cell counting kit 8 (CCK‑8) on living cells. Apoptotic morphological features and proteins expression levels were detected by Hoechst 33258 staining, an Annexin V/propidium iodide apoptosis kit and western blot analysis, respectively. Tumor xenografts were established in nude mice and the inhibitory effects and side effects were detected. Results of the CCK‑8, Hoechst 33258 staining and flow cytometry revealed that the combined treatment was more effective than the separate treatments. The tumor volume was 90.65% of that of the controls and the mean tumor weight was only 0.125 g at the end of the experiment in the combination group compared with the control group (0.822 g). In addition, it was determined that liver and renal toxicity did not increase in combined treatment. These findings showed that puerarin and 5‑FU produced a significant synergic effect on gastric cancer cells, while there was no increase in side effects.

Show MeSH
Related in: MedlinePlus