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Interaction of micron and nano-sized particles with cells of the dura mater.

Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E - J. Biomed. Mater. Res. Part B Appl. Biomater. (2014)

Bottom Line: The adverse effects of TDR wear have the potential to manifest in the dura mater and surrounding tissues.However, dural fibroblasts engulfed micron-sized particles at a much higher rate than dural epithelial cells.The study suggested that dural epithelial cells may offer some barrier to the penetration of micron-sized particles but not nanometer sized particles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical & Biological Engineering (IMBE), Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

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Representative confocal microscopy images of cells exposed to green fluorescent nano-particles (40 nm in diameter). (A–C) Dural epithelial cells. (D–F) Dural fibroblasts. (A, D) Cells exposed for 1 day. (B, E) Cells exposed for 2 days. (C, F) Cells exposed for 3 days. White arrows indicate particles internalised whereas white dashed arrows indicate particles outside from cells. After day 2 there is evidence of particle agglomeration. The actin filaments were stained with rhodamine phalloidin and the cell nuclei with sytox green. All the images were taken at 630× magnification. In total 300 cells were imaged (three replicates × 100) at each time point. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
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fig05: Representative confocal microscopy images of cells exposed to green fluorescent nano-particles (40 nm in diameter). (A–C) Dural epithelial cells. (D–F) Dural fibroblasts. (A, D) Cells exposed for 1 day. (B, E) Cells exposed for 2 days. (C, F) Cells exposed for 3 days. White arrows indicate particles internalised whereas white dashed arrows indicate particles outside from cells. After day 2 there is evidence of particle agglomeration. The actin filaments were stained with rhodamine phalloidin and the cell nuclei with sytox green. All the images were taken at 630× magnification. In total 300 cells were imaged (three replicates × 100) at each time point. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]

Mentions: The capacity of the dural cells to internalize micron (1 µm) and nano-sized (40 nm) particles was assessed over a period of 1 and 3 days. Representative examples of particle uptake are shown in Figures 3 and Figure 5 for the micron and nano-sized particles respectively.


Interaction of micron and nano-sized particles with cells of the dura mater.

Papageorgiou I, Marsh R, Tipper JL, Hall RM, Fisher J, Ingham E - J. Biomed. Mater. Res. Part B Appl. Biomater. (2014)

Representative confocal microscopy images of cells exposed to green fluorescent nano-particles (40 nm in diameter). (A–C) Dural epithelial cells. (D–F) Dural fibroblasts. (A, D) Cells exposed for 1 day. (B, E) Cells exposed for 2 days. (C, F) Cells exposed for 3 days. White arrows indicate particles internalised whereas white dashed arrows indicate particles outside from cells. After day 2 there is evidence of particle agglomeration. The actin filaments were stained with rhodamine phalloidin and the cell nuclei with sytox green. All the images were taken at 630× magnification. In total 300 cells were imaged (three replicates × 100) at each time point. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4336564&req=5

fig05: Representative confocal microscopy images of cells exposed to green fluorescent nano-particles (40 nm in diameter). (A–C) Dural epithelial cells. (D–F) Dural fibroblasts. (A, D) Cells exposed for 1 day. (B, E) Cells exposed for 2 days. (C, F) Cells exposed for 3 days. White arrows indicate particles internalised whereas white dashed arrows indicate particles outside from cells. After day 2 there is evidence of particle agglomeration. The actin filaments were stained with rhodamine phalloidin and the cell nuclei with sytox green. All the images were taken at 630× magnification. In total 300 cells were imaged (three replicates × 100) at each time point. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]
Mentions: The capacity of the dural cells to internalize micron (1 µm) and nano-sized (40 nm) particles was assessed over a period of 1 and 3 days. Representative examples of particle uptake are shown in Figures 3 and Figure 5 for the micron and nano-sized particles respectively.

Bottom Line: The adverse effects of TDR wear have the potential to manifest in the dura mater and surrounding tissues.However, dural fibroblasts engulfed micron-sized particles at a much higher rate than dural epithelial cells.The study suggested that dural epithelial cells may offer some barrier to the penetration of micron-sized particles but not nanometer sized particles.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical & Biological Engineering (IMBE), Faculty of Biological Sciences, University of Leeds, Leeds, LS2 9JT, UK.

Show MeSH
Related in: MedlinePlus