Microglial VPAC1R mediates a novel mechanism of neuroimmune-modulation of hippocampal precursor cells via IL-4 release.
Bottom Line: Here, we show that depleting microglia from hippocampal cultures reduces NSPC survival and proliferation.VIP, a neuropeptide released by dentate gyrus interneurons, enhances the proliferative and pro-neurogenic effect of microglia via the VPAC1 receptor.This VIP-induced enhancement is mediated by IL-4 release, which directly targets NSPCs.
Affiliation: Division of Clinical Neurosciences, University of Southampton, Southampton, United Kingdom.Show MeSH
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Mentions: Microglia are known to release soluble factors that affect NSPC survival and proliferation (Cacci et al., 2008; Ekdahl et al., 2009). IL-4 is a key immune cell derived cytokine that can promote learning and memory (Derecki et al., 2010). Using quantitative PCR we found that treating microglia with 30 nM VIP resulted in a fivefold increase in mRNA expression of interleukin-4 (Fig. 6A).
Affiliation: Division of Clinical Neurosciences, University of Southampton, Southampton, United Kingdom.