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Fingerprint analysis of processed Rhizoma Chuanxiong by high-performance liquid chromatography coupled with diode array detection.

Fang JY, Zhu L, Yi T, Zhang JY, Yi L, Liang ZT, Xia L, Feng JF, Xu J, Tang YN, Zhao ZZ, Chen HB - Chin Med (2015)

Bottom Line: The similarities between the seven PRC batches were range from 0.956 to 0.990.After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, Hong Kong, People's Republic of China.

ABSTRACT

Background: Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method.

Methods: A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A).

Results: In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.

Conclusions: The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.

No MeSH data available.


HPLC-DAD fingerprints of seven batches of PRC and SMC at 280 nm.
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Fig3: HPLC-DAD fingerprints of seven batches of PRC and SMC at 280 nm.

Mentions: The chromatograms of the PRC samples from the seven processing methods and the SMC were shown in Figure 3. The results of the similarity analysis were listed in Table 4. The similarities between the seven batches of PRC samples were found to range from 0.956 to 0.990 (with PRC-0 serving as a reference), and the influences of processing on RC were as follows: PRC-5 (stir-frying with vinegar) > PRC-4 (steaming with wine) > PRC-2 (steaming) > PRC-1 (stir-frying) > PRC-6 (steaming with vinegar) > PRC-3 (stir-frying with wine) > PRC-0 (raw material). From these results, the processing methods of stir-frying with vinegar and steaming with wine caused significant differences in the chemical composition of RC after processing. From Table 3, the RPA values of the 15 constituents (with peak 7 assigned as a reference) in the various PRC samples all increased, compared with those in the raw material, indicating that the amounts of the 15 compounds in RC increased after processing.Figure 3


Fingerprint analysis of processed Rhizoma Chuanxiong by high-performance liquid chromatography coupled with diode array detection.

Fang JY, Zhu L, Yi T, Zhang JY, Yi L, Liang ZT, Xia L, Feng JF, Xu J, Tang YN, Zhao ZZ, Chen HB - Chin Med (2015)

HPLC-DAD fingerprints of seven batches of PRC and SMC at 280 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4336472&req=5

Fig3: HPLC-DAD fingerprints of seven batches of PRC and SMC at 280 nm.
Mentions: The chromatograms of the PRC samples from the seven processing methods and the SMC were shown in Figure 3. The results of the similarity analysis were listed in Table 4. The similarities between the seven batches of PRC samples were found to range from 0.956 to 0.990 (with PRC-0 serving as a reference), and the influences of processing on RC were as follows: PRC-5 (stir-frying with vinegar) > PRC-4 (steaming with wine) > PRC-2 (steaming) > PRC-1 (stir-frying) > PRC-6 (steaming with vinegar) > PRC-3 (stir-frying with wine) > PRC-0 (raw material). From these results, the processing methods of stir-frying with vinegar and steaming with wine caused significant differences in the chemical composition of RC after processing. From Table 3, the RPA values of the 15 constituents (with peak 7 assigned as a reference) in the various PRC samples all increased, compared with those in the raw material, indicating that the amounts of the 15 compounds in RC increased after processing.Figure 3

Bottom Line: The similarities between the seven PRC batches were range from 0.956 to 0.990.After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.

View Article: PubMed Central - PubMed

Affiliation: School of Chinese Medicine, Hong Kong Baptist University, Hong Kong Special Administrative Region, Hong Kong, People's Republic of China.

ABSTRACT

Background: Rhizoma Chuanxiong (RC) is the dried rhizome of Ligusticum chuanxiong Hort., and various types of processed Rhizoma Chuanxiong (PRC) are widely used in China. However, quality assurance and quality control of these processed medicines remain challenging. This study aims to investigate the chemical compositions of various PRC preparations by a high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) method.

Methods: A HPLC-DAD method with validation was developed for PRC samples. Seven batches of plant samples from two processing methods, stir-frying and steaming, were analyzed by the HPLC-DAD method. Common peaks in PRC chromatograms were chosen to calculate their relative retention time (RRT) and relative peak area (RPA), and similarity analyses of the chromatographic fingerprints were conducted by Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine software (Version 2004 A).

Results: In the 24-h stability test, the relative standard deviation for the RRT and RPA was less than 0.07% and 2.57%, respectively. The precision was less than 0.08% for the RRT and 2.48% for the RPA. The repeatability for the RRT and RPA was less than 0.03% and 2.64%, respectively. The similarities between the seven PRC batches were range from 0.956 to 0.990. After stir-frying or steaming, the amount of ferulic acid in PRC was much higher than that in the raw material.

Conclusions: The fingerprint analysis of PRC by different processing methods was feasible by HPLC-DAD.

No MeSH data available.