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Soybean (Glycine max L. Merr.) sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease.

Dhakal R, Park E, Lee SW, Baek KH - PLoS ONE (2015)

Bottom Line: Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence.To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs.Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, Republic of Korea.

ABSTRACT
Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence. To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs. The controls were composed of soybean sprouts germinated in darkness. After germination under different conditions for 5 days, the soybean sprouts were inoculated with P. putida 229 and the disease incidence was observed for 5 days. The sprouts exposed to red light showed increased resistance against P. putida 229 relative to those grown under other conditions. Soybean sprouts germinated under red light accumulated high levels of salicylic acid (SA) accompanied with up-regulation of the biosynthetic gene ICS and the pathogenesis- related (PR) gene PR-1, indicating that the resistance was induced by the action of SA via de novo synthesis of SA in the soybean sprouts by red light irradiation. Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

No MeSH data available.


Related in: MedlinePlus

Effects of different wavelengths on the growth of soybean sprouts and disease susceptibility to Pseudomonas putida 229.Soybean sprouts were germinated for 5 days under different conditions. (A) The soybean sprouts germinated in darkness, red light, far-red light, blue light and fluorescence daylight. The sprouts tips were cut, inoculated in bacterial culture for 8 h, and then incubated for 5 days in darkness. The disease symptoms were observed after 3 and 5 days of incubation in darkness. (B) Comparison of disease incidence in soybean sprouts germinated in darkness and under different light irradiation for 5 days. The experiments were repeated four times. The different letter indicates a significant difference (P<0.01).
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pone.0117712.g001: Effects of different wavelengths on the growth of soybean sprouts and disease susceptibility to Pseudomonas putida 229.Soybean sprouts were germinated for 5 days under different conditions. (A) The soybean sprouts germinated in darkness, red light, far-red light, blue light and fluorescence daylight. The sprouts tips were cut, inoculated in bacterial culture for 8 h, and then incubated for 5 days in darkness. The disease symptoms were observed after 3 and 5 days of incubation in darkness. (B) Comparison of disease incidence in soybean sprouts germinated in darkness and under different light irradiation for 5 days. The experiments were repeated four times. The different letter indicates a significant difference (P<0.01).

Mentions: All soybean seeds germinated under the aforementioned conditions; however, they developed different colors and hypocotyl growth (Fig. 1A). The sprouts germinated under red, blue and white light were dark green, while those germinated under far-red light were light green (Fig. 1A). The sprouts germinated in darkness had yellow seeds and white hypocotyls due to the lack of chlorophylls in the absence of photosynthesis. Additionally, the sprouts germinated under far-red light had shorter hypocotyls than those grown under other conditions (Fig. 1A).


Soybean (Glycine max L. Merr.) sprouts germinated under red light irradiation induce disease resistance against bacterial rotting disease.

Dhakal R, Park E, Lee SW, Baek KH - PLoS ONE (2015)

Effects of different wavelengths on the growth of soybean sprouts and disease susceptibility to Pseudomonas putida 229.Soybean sprouts were germinated for 5 days under different conditions. (A) The soybean sprouts germinated in darkness, red light, far-red light, blue light and fluorescence daylight. The sprouts tips were cut, inoculated in bacterial culture for 8 h, and then incubated for 5 days in darkness. The disease symptoms were observed after 3 and 5 days of incubation in darkness. (B) Comparison of disease incidence in soybean sprouts germinated in darkness and under different light irradiation for 5 days. The experiments were repeated four times. The different letter indicates a significant difference (P<0.01).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4334547&req=5

pone.0117712.g001: Effects of different wavelengths on the growth of soybean sprouts and disease susceptibility to Pseudomonas putida 229.Soybean sprouts were germinated for 5 days under different conditions. (A) The soybean sprouts germinated in darkness, red light, far-red light, blue light and fluorescence daylight. The sprouts tips were cut, inoculated in bacterial culture for 8 h, and then incubated for 5 days in darkness. The disease symptoms were observed after 3 and 5 days of incubation in darkness. (B) Comparison of disease incidence in soybean sprouts germinated in darkness and under different light irradiation for 5 days. The experiments were repeated four times. The different letter indicates a significant difference (P<0.01).
Mentions: All soybean seeds germinated under the aforementioned conditions; however, they developed different colors and hypocotyl growth (Fig. 1A). The sprouts germinated under red, blue and white light were dark green, while those germinated under far-red light were light green (Fig. 1A). The sprouts germinated in darkness had yellow seeds and white hypocotyls due to the lack of chlorophylls in the absence of photosynthesis. Additionally, the sprouts germinated under far-red light had shorter hypocotyls than those grown under other conditions (Fig. 1A).

Bottom Line: Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence.To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs.Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

View Article: PubMed Central - PubMed

Affiliation: School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, Republic of Korea.

ABSTRACT
Specific wavelengths of light can exert various physiological changes in plants, including effects on responses to disease incidence. To determine whether specific light wavelength had effects on rotting disease caused by Pseudomonas putida 229, soybean sprouts were germinated under a narrow range of wavelengths from light emitting diodes (LEDs), including red (650-660), far red (720-730) and blue (440-450 nm) or broad range of wavelength from daylight fluorescence bulbs. The controls were composed of soybean sprouts germinated in darkness. After germination under different conditions for 5 days, the soybean sprouts were inoculated with P. putida 229 and the disease incidence was observed for 5 days. The sprouts exposed to red light showed increased resistance against P. putida 229 relative to those grown under other conditions. Soybean sprouts germinated under red light accumulated high levels of salicylic acid (SA) accompanied with up-regulation of the biosynthetic gene ICS and the pathogenesis- related (PR) gene PR-1, indicating that the resistance was induced by the action of SA via de novo synthesis of SA in the soybean sprouts by red light irradiation. Taken together, these data suggest that only the narrow range of red light can induce disease resistance in soybean sprouts, regulated by the SA-dependent pathway via the de novo synthesis of SA and up-regulation of PR genes.

No MeSH data available.


Related in: MedlinePlus