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F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression.

Mo X, Yang C, Wang X, Burkhardt BR, Li Y, Xia H, Cao X - PLoS ONE (2015)

Bottom Line: The TG metabolism in both groups were compared.Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes.Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.

ABSTRACT

Objective: Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice.

Methods: PANDER overexpression was achieved by tail-vein injection of recombinant Ad-PANDER and Ad-GFP injected mice served as a control. The TG metabolism in both groups were compared.

Results: Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes. The triglyceride (TG) content of both liver and adipose tissue was significantly decreased in Ad-PANDER mice (liver: 6.16±1.89 mg/g vs. control 14.95±2.27 mg/g, P<0.05; adipose: 39.31±1.99 mg/100mg vs. 47.22±2.21 mg/100mg, P<0.05). The free fatty acid (FFA) content of adipose tissue in Ad-PANDER mice was also decreased (1.38±0.18 mg/g vs. 2.77±0.31 mg/g, P<0.01). The investigation of key enzymes of triglyceride hydrolysis and FFA oxidation in liver and adipose tissue showed that p-HSL/HSL was significantly increased and that DGAT1 gene and protein expression were significantly reduced in the liver of PANDER-overexpressing mice. PKA phosphorylation was also significantly increased in the livers of Ad-PANDER mice. No differences in ATGL, CPT1, ACOX1, or DGAT2 expression were observed.

Conclusion: Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship. The mechanisms by which this occurs remain to be elucidated.

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Effects of PANDER overexpression on key enzymes of triglyceride hydrolysis in liver and adipose tissue of mice.Total HSL, p-HSL, and ATGL were measured in liver (Fig. 4A) and adipose tissue (Fig. 4B) by western blot 14 days after adenovirus transfection. The results are presented of five independent samples.
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pone.0117156.g004: Effects of PANDER overexpression on key enzymes of triglyceride hydrolysis in liver and adipose tissue of mice.Total HSL, p-HSL, and ATGL were measured in liver (Fig. 4A) and adipose tissue (Fig. 4B) by western blot 14 days after adenovirus transfection. The results are presented of five independent samples.

Mentions: To explore the possible mechanism underlying the effects of PANDER on TG metabolism, we measured the total and phosphorylated levels of key enzymes of triglyceride hydrolysis in liver and adipose tissue. Among triglyceride lipases, HSL and ATGL are the two essential lipases [17]. We measured the expression of ATGL, HSL, and p-HSL by western blot analysis. The results showed that p-HSL was significantly increased (p<0.05) in liver of Ad-PANDER mice. No difference in ATGL and total HSL expression in liver was observed between the Ad-PANDER mice and Ad-GFP mice (Fig. 4A). The expression of p-HSL, HSL, and ATGL in adipose tissue also did not differ significantly between the two groups (Fig. 4B).


F3MB(PANDER) decreases mice hepatic triglyceride and is associated with decreased DGAT1 expression.

Mo X, Yang C, Wang X, Burkhardt BR, Li Y, Xia H, Cao X - PLoS ONE (2015)

Effects of PANDER overexpression on key enzymes of triglyceride hydrolysis in liver and adipose tissue of mice.Total HSL, p-HSL, and ATGL were measured in liver (Fig. 4A) and adipose tissue (Fig. 4B) by western blot 14 days after adenovirus transfection. The results are presented of five independent samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4334525&req=5

pone.0117156.g004: Effects of PANDER overexpression on key enzymes of triglyceride hydrolysis in liver and adipose tissue of mice.Total HSL, p-HSL, and ATGL were measured in liver (Fig. 4A) and adipose tissue (Fig. 4B) by western blot 14 days after adenovirus transfection. The results are presented of five independent samples.
Mentions: To explore the possible mechanism underlying the effects of PANDER on TG metabolism, we measured the total and phosphorylated levels of key enzymes of triglyceride hydrolysis in liver and adipose tissue. Among triglyceride lipases, HSL and ATGL are the two essential lipases [17]. We measured the expression of ATGL, HSL, and p-HSL by western blot analysis. The results showed that p-HSL was significantly increased (p<0.05) in liver of Ad-PANDER mice. No difference in ATGL and total HSL expression in liver was observed between the Ad-PANDER mice and Ad-GFP mice (Fig. 4A). The expression of p-HSL, HSL, and ATGL in adipose tissue also did not differ significantly between the two groups (Fig. 4B).

Bottom Line: The TG metabolism in both groups were compared.Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes.Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship.

View Article: PubMed Central - PubMed

Affiliation: Department of Endocrinology, First Affiliated Hospital, Sun Yat-sen University, No.58 Zhongshan 2nd Road, Guangzhou 510080, PR.China.

ABSTRACT

Objective: Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice.

Methods: PANDER overexpression was achieved by tail-vein injection of recombinant Ad-PANDER and Ad-GFP injected mice served as a control. The TG metabolism in both groups were compared.

Results: Adenoviral-mediated overexpression of PANDER did not affect body weight, food consumption, or liver enzymes. The triglyceride (TG) content of both liver and adipose tissue was significantly decreased in Ad-PANDER mice (liver: 6.16±1.89 mg/g vs. control 14.95±2.27 mg/g, P<0.05; adipose: 39.31±1.99 mg/100mg vs. 47.22±2.21 mg/100mg, P<0.05). The free fatty acid (FFA) content of adipose tissue in Ad-PANDER mice was also decreased (1.38±0.18 mg/g vs. 2.77±0.31 mg/g, P<0.01). The investigation of key enzymes of triglyceride hydrolysis and FFA oxidation in liver and adipose tissue showed that p-HSL/HSL was significantly increased and that DGAT1 gene and protein expression were significantly reduced in the liver of PANDER-overexpressing mice. PKA phosphorylation was also significantly increased in the livers of Ad-PANDER mice. No differences in ATGL, CPT1, ACOX1, or DGAT2 expression were observed.

Conclusion: Overexpression of PANDER is associated with observable decreases in TG, increases in PKA phosphorylation, and decreased DGAT1 expression, suggesting a possible interrelationship. The mechanisms by which this occurs remain to be elucidated.

Show MeSH
Related in: MedlinePlus